The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
Open accessAbstract
ABSTRACT Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200–5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 10 3 copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 × 10 3 –8.2 × 10 4 copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 × 10 2 –2.5 × 10 3 copies/L) with a higher filtration volume of 5,000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.
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Stability of SARS-CoV-2 in different environmental conditions
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Cited by
Kumar, M., Patel, A. K., Shah, A. V., Raval, J., Rajpara, N., Joshi, M., & Joshi, C. G. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.141326 · Cited by 474
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Viruses in wastewater: occurrence, abundance and detection methods
Corpuz, M. V. A., Buonerba, A., Vigliotta, G., Zarra, T., Ballesteros, F., Campiglia, P., Belgiorno, V., Korshin, G., & Naddeo, V. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.140910 · Cited by 325
Rethinking wastewater risks and monitoring in light of the COVID-19 pandemic
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Metadata
- Authors
- Haramoto, E., Malla, B., Thakali, O., & Kitajima, M.
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2020-10
- Volume
- 737
- Pages
- 140405
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W3036431624
- Cited by
- 593
- References
- 41
- FWCI
- 14.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Haramoto, E., Malla, B., Thakali, O., & Kitajima, M. (2020). First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan. Science of The Total Environment. https://doi.org/10.1016/j.scitotenv.2020.140405
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
