Inoculation of plant growth promoting-methane utilizing bacteria in different N-fertilizer regime influences methane emission and crop growth of flooded paddy
Abstract
Methane (CH 4) emission in rice fields is greatly influenced by the type and quantity of nitrogenous fertilizer used. The net methane emission from paddy fields is also influenced by the activity of methane utilizing bacteria, which inhabit the flooded paddy ecosystem. Efficient methane utilizing and plant growth promoting bacteria Methylobacterium oryzae MNL7 and Paenibacillus polymyxa MaAL70, respectively were co-inoculated along with different nitrogenous fertilizer combinations in flooded paddy to assess their impact on cumulative methane emission and crop growth promotion. Co-inoculation significantly influenced the plant growth parameters of paddy, resulting in an increase in grain yield by 14.04, 11.08, and 12.38% in treatments receiving Urea, Di-ammonium Phosphate (DAP) + Urea, or farm yard manure (FYM), over their respective un-inoculated plots. Significant improvement in the rice grain nutrient quality in term of crude protein, Fe and Zn content was observed as a result of bacterial co-inoculation in FYM fertilized plots as compared to Urea and DAP+ Urea fertilized plots. Significantly higher cumulative methane emission of 63.39 kg ha-1 was observed in uninoculated plots fertilized with FYM treatment as compared to Urea (33.83 kg ha-1) and DAP+Urea (31.66 kg ha-1) treatments. Bacterial co-inoculation significantly reduced the cumulative methane emission by 12.03, 11.47 and 6.92% in Urea, DAP+Urea, and FYM fertilized plots over their respective uninoculated treatments. Among the different fertilizer treatments, bacterial co-inoculation with urea application performed significantly better in reducing cumulative methane emission. These findings suggest that methane utilizing bacteria which also possess plant growth promoting trait can be explored for developing a novel biofertilizer for flooded paddies, as they can aid in managing both the overall methane emission and enhancing crop yield.
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Development of inhibitors for methanogenesis or addition of biochar in rice paddies has large technical potential to reduce CH4 emissions.
“the development of inhibitors for methanogenesis or the addition of biochar in rice paddies has a large technical potential to reduce CH4 emissions.121,122”
From Technologies and perspectives for achieving carbon neutrality (2021) · cites this paper as [122]
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Khokhar, N. H., & Park, J. (2017) · Environmental Monitoring and Assessment · doi:10.1007/s10661-017-6184-z · Cited by 5 · see citing works
Tejaswini, M., Sreedevi, B., V, & Akula, B. (2017) · Cited by 5 · see citing works
Isolation And Characterization Of Methylotrophic Bacteria From Western Ghats
Rajasekar, R. G. D. A. A. (2013) · Cited by 4 · see citing works
Bacteria as Greenhouse Gases Reducing Agents from Paddy Plantation
Muttaqin, M., Miftahudin, M., & Rusmana, İ. (2017) · Jurnal Sumberdaya Hayati · doi:10.29244/jsdh.2.2.45-51 · Cited by 3 · see citing works
Analysis of Potensial Reduce Methane Gas Emission by Methanotrophs Bacteria from Rice Field in Gowa
Nonci, M., Baharuddin, B., Rasyid, B., et al. (2016) · Hasanuddin University Repository · Cited by 2 · see citing works
Athiappan, M., Rubavathi, A., Visali, K., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.03.16.994640 · Cited by 2 · see citing works
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Technologies and perspectives for achieving carbon neutrality
Wang, F., Harindintwali, J. D., Yuan, Z., Wang, M., Wang, F., Li, S., Yin, Z., Huang, L., Fu, Y., Li, L., et al. (2021) · The Innovation · doi:10.1016/j.xinn.2021.100180 · Cited by 1,330 · see citing works
- Authors
- Rani, V., Bhatia, A., & Kaushik, R.
- Affiliations
- Indian Agricultural Research Institute (IN)
- Indian Council of Agricultural Research (IN)
- Corresponding author
- Kaushik, Rajeev
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2021-06
- Volume
- 775
- Pages
- 145826
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W3132017300
- Cited by
- 60
- References
- 119
- Citations by year
- 2026: 14 · 2025: 12 · 2024: 12 · 2023: 10 · 2022: 6 · 2021: 6
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Council of Agricultural Research
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Agricultural Science and Fertilization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Rice Cultivation and Yield Improvement
- Keywords
- methane emissions, rice, flooded paddy, methane-utilizing bacteria, plant growth-promoting bacteria, Methylobacterium oryzae, Paenibacillus polymyxa, nitrogen fertilization, urea, diammonium phosphate, farmyard manure, biofertilizers, grain yield, crude protein, iron and zinc content
- MeSH terms
- Agriculture, Fertilizers, Methane, Nitrous Oxide, Oryza, Soil, Ecosystem, Methylobacterium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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