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Co-occurrence of antimicrobial and metal resistance genes in pig feces and agricultural fields fertilized with slurry

Peng, Shifu, Zheng, Hao, Herrero-Fresno, Ana, Olsen, John E., Dalsgaard, Anders, Ding, Zhen·2021·Science of The Total Environment
DOI 10.1016/j.scitotenv.2021.148259
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Abstract

Antimicrobial resistance constitutes a global challenge to public health. The common addition of Zn, Cu and other metals to animal feed and the widespread presence of metal ions in livestock and their receiving environments may be a factor that facilitates the proliferation of antimicrobial resistance via co-selection of antimicrobial resistance genes (ARGs) and metal resistance genes (MRGs). However, the extent of co-selection is not yet fully understood. In this study, we used a metagenomic approach to profile ARGs, MRGs and mobile genetic elements (MGEs) known to constitute potential ARG and MRG vectors of transmission, and we determined the concentration of metal ions to assess the interrelationships between the occurrence of ARGs, MRGs and metal concentrations in samples from pig farms in China. Samples analyzed included fresh pig feces, soils fertilized with treated slurry, and sediments from aquatic environments, where effluent from treated slurry was discharged. Resistance genes to tetracycline and zinc were the most commonly observed ARGs and MRGs for all three types of samples. Significant correlations were observed between the abundance of ARGs and MRGs, and between ARGs/MRGs and MGEs, and between metal and ARGs/MGEs as documented by Pearson's correlation analysis (r > 0.9, P < 0.001). Further network analysis revealed significant co-occurrence between specific ARGs and MRGs, between ARGs/MRGs and MGEs, and between specific metals (Zn, Cr, and Mn) and ARGs and MGEs…

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Metadata

Authors
Peng, S., Zheng, H., Herrero-Fresno, A., Olsen, J. E., Dalsgaard, A., & Ding, Z.
Venue
Science of The Total Environment
Publisher
Elsevier BV
Published
2021-10
Volume
792
Pages
148259
ISSN
0048-9697
DOI
10.1016/j.scitotenv.2021.148259
Cited by
51
References
3
Language
en
Licence
Funders
China Scholarship Council

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