Cells with surface expression of CD133highCD71low are enriched for tripotent colony-forming progenitor cells in the adult murine pancreas
Open accessAbstract
Progenitor cells in the adult pancreas are potential sources of endocrine beta cells for treating type 1 diabetes. Previously, we identified tri-potent progenitor cells in the adult (2-4month-old) murine pancreas that were capable of self-renewal and differentiation into duct, acinar, and endocrine cells in vitro. These progenitor cells were named pancreatic colony-forming units (PCFUs). However, because PCFUs are a minor population in the pancreas (~1%) they are difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses and fluorescence-activated cell sorting were developed. We found that CD133(high)CD71(low) cells, but not other cell populations, enriched PCFUs by up to 30 fold compared to the unsorted cells. CD133(high)CD71(low) cells generated primary, secondary, and subsequent colonies when serially re-plated in Matrigel-containing cultures, suggesting self-renewal abilities. In the presence of a laminin hydrogel, CD133(high)CD71(low) cells gave rise to colonies that contained duct, acinar, and Insulin(+)Glucagon(+) double-hormonal endocrine cells. Colonies from the laminin hydrogel culture were implanted into diabetic mice, and five weeks later duct, acinar, and Insulin(+)Glucagon(-) cells were detected in the grafts, demonstrating tri-lineage differentiation potential of CD133(high)CD71(low) cells. These CD133(high)CD71(low) cells will enable future studies of putative adult pancreas stem cells in vivo.
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The exact population of adult progenitor cells capable of giving rise to endocrine/acinar cells in aECM-lam was determined to be ductal cells, which have high levels of CD133 but low levels of CD71 expression.
“Finally, the exact population of adult progenitor cells capable of giving rise to endocrine/acinar cells in aECM-lam was determined to be ductal cells, which have high levels of CD133 but low levels of CD71 expression195.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [195]
Using aECM-lam, other morphologically-distinct organoids were formed from murine postnatal pancreas and sorted adult ductal progenitor cells.
“Using aECM-lam, other morphologically-distinct organoids were formed from murine postnatal pancreas194 and sorted adult ductal progenitor cells195.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [195]
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Towards organoid culture without Matrigel
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- Authors
- Jin, L., Gao, D., Feng, T., Tremblay, J. R., Ghazalli, N., Luo, A., Rawson, J., Quijano, J. C., Chai, J., Wedeken, L., Hsu, J., LeBon, J., Walker, S., Shih, H.-P., Mahdavi, A., Tirrell, D. A., Riggs, A. D., & Ku, H. T.
- Affiliations
- City Of Hope National Medical Center (US)
- California Institute of Technology (US)
- Corresponding author
- Ku, H. Teresa
- ORCID
- Venue
- Stem Cell Research
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 16
- Issue
- 1
- Pages
- 40-53
- ISSN
- 1873-5061 (ISSN-L)
- PMC
- PMC4762724
- OpenAlex
- W2186222783
- Cited by
- 32
- References
- 45
- Citations by year
- 2025: 4 · 2024: 5 · 2022: 3 · 2021: 5 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 3
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion, cc-by-nc-nd)
- Funders
- National Cancer Institute of the National Institutes of Health (P30CA33572)
- National Institutes of Health (R01DK081587, R01DK099734, U01DK089533)
- National Science Foundation (NSF-DMR-1206121)
- California Institute for Regenerative Medicine (RB5-07398)
- Office of Naval Research (ONR-N00014-02-1 0958, NSF-DBI-9970143)
- National High Technology Research and Development Program of China (2015AA020314)
- Excellent Youth Foundation of Jiangsu Scientific Committee (BK20140029)
- Fundamental Research Funds for the Central Universities (Z114037)
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- National Natural Science Foundation of China (81570696)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Keywords
- pancreatic progenitor cells, CD133, transferrin receptor, adult pancreas, type 1 diabetes, self-renewal, multilineage differentiation, glucagon, cell surface markers, pancreatic stem cells
- MeSH terms
- Cell Self Renewal, AC133 Antigen, Aging, Animals, Cell Membrane, Colony-Forming Units Assay, Glucagon, Immunohistochemistry, Insulin, Mice, Inbred C57BL, Pancreas, Pancreatic Ducts, Receptors, Transferrin, Antigens, CD, Paraffin Embedding, Tissue Fixation, Sequence Analysis, RNA, Multipotent Stem Cells, Acinar Cells
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