Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel
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The study of hematopoietic colony-forming units using semisolid culture media has greatly advanced the knowledge of hematopoiesis. Here we report that similar methods can be used to study pancreatic colony-forming units. We have developed two pancreatic colony assays that enable quantitative and functional analyses of progenitor-like cells isolated from dissociated adult (2–4 mo old) murine pancreas. We find that a methylcellulose-based semisolid medium containing Matrigel allows growth of duct-like “Ring/Dense” colonies from a rare ( ∼ 1%) population of total pancreatic single cells. With the addition of roof plate-specific spondin 1, a wingless-int agonist, Ring/Dense colony-forming cells can be expanded more than 100,000-fold when serially dissociated and replated in the presence of Matrigel. When cells grown in Matrigel are then transferred to a Matrigel-free semisolid medium with a unique laminin-based hydrogel, some cells grow and differentiate into another type of colony, which we name “Endocrine/Acinar.” These Endocrine/Acinar colonies are comprised mostly of endocrine- and acinar-like cells, as ascertained by RNA expression analysis, immunohistochemistry, and electron microscopy. Most Endocrine/Acinar colonies contain beta-like cells that secrete insulin/C-peptide in response to D -glucose and theophylline. These results demonstrate robust self-renewal and differentiation of adult Ring/Dense colony-forming units in vitro and suggest an approach to producing beta-like cells for cell replacement of type 1 diabetes. The methods described, which include microfluidic expression analysis of single cells and colonies, should also advance study of pancreas development and pancreatic progenitor cells.
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After 2 weeks, endocrine-acinar organoids were observed, demonstrating that aECM-lam was capable of inducing differentiation of ductal progenitor cells into endocrine and acinar lineages.
“After 2 weeks, endocrine-acinar organoids were observed, demonstrating that aECM-lam was capable of inducing differentiation of ductal progenitor cells into endocrine and acinar lineages192.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [192]
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- Authors
- Jin, L., Feng, T., Shih, H. P., Zerda, R., Luo, A., Hsu, J., Mahdavi, A., Sander, M., Tirrell, D. A., Riggs, A. D., & Ku, H. T.
- Affiliations
- Department of Diabetes and Metabolic Diseases Research,
- University of California San Diego (US)
- Electron Microscopy Core, and
- Department of Bioengineering, and
- California Institute of Technology (US)
- City Of Hope National Medical Center (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2013-02-19
- Volume
- 110
- Issue
- 10
- Pages
- 3907-3912
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3593860
- OpenAlex
- W2160112404
- Cited by
- 108
- References
- 60
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 11 · 2017: 8 · 2016: 14 · 2015: 9 · 2014: 15 · 2013: 6
- FWCI
- 14.45
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
- Funders
- National Cancer Institute (P30CA-33572, P30CA033572)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK078803, R01DK081587, R01DK078803, U01DK089533)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Keywords
- pancreatic progenitor cells, Matrigel, beta-like cells, insulin secretion, self-renewal, pancreatic development, semi-solid culture
- MeSH terms
- Animals, Cell Differentiation, Collagen, Colony-Forming Units Assay, Culture Media, Drug Combinations, Islets of Langerhans, Laminin, Mice, Inbred C57BL, Mice, Transgenic, Nerve Tissue Proteins, Pancreas, Proteoglycans, Hydrogels, Microscopy, Electron, Transmission, Insulin-Secreting Cells, Mice, Basic Helix-Loop-Helix Proteins, Wnt Signaling Pathway, Acinar Cells
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