Postnatal Pancreas of Mice Contains Tripotent Progenitors Capable of Giving Rise to Duct, Acinar, and Endocrine Cells In Vitro

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204 · see citing works

Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen

Shapiro, A. M. J., Lakey, J. R. T., Ryan, E. A., Korbutt, G. S., Toth, E., Warnock, G. L., Kneteman, N. M., & Rajotte, R. V. (2000) · New England Journal of Medicine · doi:10.1056/nejm200007273430401 · Cited by 5,237 · see citing works

Organoid Models and Applications in Biomedical Research

Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,517 · see citing works

Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors

Gu, G., Dubauskaite, J., & Melton, D. A. (2002) · Development · doi:10.1242/dev.129.10.2447 · Cited by 1,518 · see citing works

neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas

Gradwohl, G., Dierich, A., LeMeur, M., & Guillemot, F. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.4.1607 · Cited by 1,435 · see citing works

The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors

Kawaguchi, Y., Cooper, B., Gannon, M., Ray, M., MacDonald, R. J., & Wright, C. V. E. (2002) · Nature Genetics · doi:10.1038/ng959 · Cited by 1,007 · cited 1× · see citing works

A simplified laminin nomenclature

Aumailley, M., Bruckner‐Tuderman, L., Carter, W. G., Deutzmann, R., EDGAR, D., Ekblom, P., Engel, J., Engvall, E., Hohenester, E., & Jones, J. (2005) · Matrix Biology · doi:10.1016/j.matbio.2005.05.006 · Cited by 928 · see citing works

Purification of recombinant proteins by fusion with thermally-responsive polypeptides

Meyer, D. E., & Chilkoti, A. (1999) · Nature Biotechnology · doi:10.1038/15100 · Cited by 914 · see citing works

Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis

Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C. J. M., van de Wetering, M., Sojoodi, M., Li, V. S. W., Schuijers, J., Gračanin, A., et al. (2013) · EMBO Journal · doi:10.1038/emboj.2013.204 · Cited by 710 · see citing works

Pancreas organogenesis: From bud to plexus to gland

Pan, F. C., & Wright, C. (2011) · Developmental Dynamics · doi:10.1002/dvdy.22584 · Cited by 638 · see citing works

Adhesive recognition sequences

Yamada, K. M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98761-2 · Cited by 571 · see citing works

Expression of murine STF-1, a putative insulin gene transcription factor, in β cells of pancreas, duodenal epithelium and pancreatic exocrine and endocrine progenitors during ontogeny

Guz, Y., Montminy, M., Stein, R. W., Leonard, J. N., Gamer, L. W., Wright, C. V. E., & Teitelman, G. (1995) · Development · doi:10.1242/dev.121.1.11 · Cited by 565 · see citing works

Molecular Architecture and Function of Matrix AdhesionsPreprint

Geiger, B., & Yamada, K. M. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005033 · Cited by 548 · see citing works

A Multipotent Progenitor Domain Guides Pancreatic Organogenesis

Zhou, Q., Law, A. C., Rajagopal, J., Anderson, W. J., Gray, P. A., & Melton, D. A. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.001 · Cited by 530 · see citing works

Pancreatic exocrine duct cells give rise to insulin-producing beta cells during embryogenesis but not after birth.

Solar, M., Cardalda, C., Houbracken, I., Martín, M., Maestro, M., Demedts, N., Xu, X.-B., Grau, V., Heimberg, H., Bouwens, L., et al. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.11.003 · Cited by 516 · see citing works

The Vascular Basement Membrane: A Niche for Insulin Gene Expression and β Cell Proliferation

Nikolova, G. V., Jabs, N., Konstantinova, I., Domogatskaya, A., Tryggvason, K., Sorokin, L. M., Fässler, R., Gu, G., Gerber, H., Ferrara, N., et al. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.01.015 · Cited by 514 · see citing works

Sox9+ ductal cells are multipotent progenitors throughout development but do not produce new endocrine cells in the normal or injured adult pancreas

Kopp, J. L., Dubois, C. L., Schaffer, A., Hao, E., Shih, H., Seymour, P. A., Ma, J., & Sander, M. (2011) · Development · doi:10.1242/dev.056499 · Cited by 472 · see citing works

Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth

Inada, A., Nienaber, C. E., Katsuta, H., Fujitani, Y., Levine, J., Morita, R., Sharma, A., & Bonner‐Weir, S. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805803105 · Cited by 447 · see citing works

Functional Diversity of Laminins

Domogatskaya, A., Rodin, S., & Tryggvason, K. (2012) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101011-155750 · Cited by 396 · see citing works

Islet architecture: A comparative study

Kim, A., Miller, K. W. P., Jo, J., Kilimnik, G., Wojcik, P., & Hara, M. (2009) · Islets · doi:10.4161/isl.1.2.9480 · Cited by 384 · see citing works

Functional β-cells maturation is marked by an increase in the glucose threshold for insulin secretion and by expression of urocortin3

Blum, B., Hrvatin, S., Schuetz, C., Bonal, C., Rezania, A., & Melton, D. (2012) · Nature Biotechnology · doi:10.1038/nbt.2141 · Cited by 349 · see citing works

Temporal Control of Neurogenin3 Activity in Pancreas Progenitors Reveals Competence Windows for the Generation of Different Endocrine Cell Types

Johansson, K., Dursun, U., Jordan, N., Gu, G., Beermann, F., Gradwohl, G., & Grapin‐Botton, A. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.02.010 · Cited by 337 · see citing works

Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas

Rovira, M., Scott, S.-G., Liss, A., Jensen, J., Thayer, S., & Leach, S. (2009) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0912589107 · Cited by 313 · see citing works

Artificial three-dimensional niches deconstruct pancreas development in vitro

Greggio, C., Franceschi, F. D., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M., & Grapin-Botton, A. (2013) · Development · doi:10.1242/dev.096628 · Cited by 291 · see citing works

Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis.

Gu, G.-Q., Brown, J. R., & Melton, D. (2003) · Mechanisms of Development · doi:10.1016/s0925-4773(02)00330-1 · Cited by 236 · see citing works

Cell–Matrix Interactions Improve β-Cell Survival and Insulin Secretion in Three-Dimensional Culture

Weber, L. M., Hayda, K. N., & Anseth, K. S. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2007.0238 · Cited by 233 · see citing works

The effects of cell–matrix interactions on encapsulated β-cell function within hydrogels functionalized with matrix-derived adhesive peptides

Weber, L. M., Hayda, K. N., HASKINS, K. M., & Anseth, K. S. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.03.005 · Cited by 224 · see citing works

Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins

Heilshorn, S. C. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00294-1 · Cited by 188 · see citing works

Laminin-5 Induces Osteogenic Gene Expression in Human Mesenchymal Stem Cells through an ERK-dependent Pathway

Klees, R. F., Salasznyk, R. M., Kingsley, K., Williams, W. A., Boskey, A. L., & Plopper, G. E. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-08-0695 · Cited by 181 · see citing works

Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues

Keung, A. J., Kumar, S., & Schaffer, D. V. (2010) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100109-104042 · Cited by 170 · see citing works

Integrin Signaling via RGD Peptides and Anti-β1 Antibodies Confers Resistance to Apoptosis in Islets of Langerhans

Pinkse, G. G. M., Bouwman, W. P., Jiawan-Lalai, R., Terpstra, O. T., Bruijn, J. A., & de Heer, E. (2006) · Diabetes · doi:10.2337/diabetes.55.02.06.db04-0195 · Cited by 162 · see citing works

Quantitative Model of the Phase Behavior of Recombinant pH-Responsive Elastin-Like Polypeptides

MacKay, J. A., Callahan, D. J., FitzGerald, K. N., & Chilkoti, A. (2010) · Biomacromolecules · doi:10.1021/bm100571j · Cited by 160 · see citing works

Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells

Lee, J., Sugiyama, T., Liu, Y.-H., Wang, J., Gu, X.-Y., Lei, J., Markmann, J., Miyazaki, S., Miyazaki, J., Szot, G., et al. (2013) · eLife · doi:10.7554/elife.00940 · Cited by 158 · see citing works

Hydrogel encapsulation environments functionalized with extracellular matrix interactions increase islet insulin secretion

Weber, L. M., & Anseth, K. S. (2008) · Matrix Biology · doi:10.1016/j.matbio.2008.08.001 · Cited by 154 · see citing works

Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking

Nowatzki, P. J., & Tirrell, D. A. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00635-5 · Cited by 151 · see citing works

Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development

Gouzi, M., Kim, Y. H., Katsumoto, K., Johansson, K., & Grapin‐Botton, A. (2011) · Developmental Dynamics · doi:10.1002/dvdy.22544 · Cited by 148 · see citing works

Isolation of Mouse Pancreatic Ductal Progenitor Cells Expressing CD133 and c-Met by Flow Cytometric Cell Sorting

Oshima, Y., Suzuki, A., Kawashimo, K., Ishikawa, M., Ohkohchi, N., & Taniguchi, H. (2006) · Gastroenterology · doi:10.1053/j.gastro.2006.11.027 · Cited by 137 · see citing works

Neurogenin3 inhibits proliferation in endocrine progenitors by inducing Cdkn1a

Miyatsuka, T., Kosaka, Y., Kim, H., & German, M. S. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004842108 · Cited by 120 · see citing works

Blockade of β1 Integrin–Laminin-5 Interaction Affects Spreading and Insulin Secretion of Rat β-Cells Attached on Extracellular Matrix

Parnaud, G., Hammar, E. B., Rouiller, D. G., Armanet, M., Halban, P. A., & Bosco, D. (2006) · Diabetes · doi:10.2337/db05-1388 · Cited by 120 · see citing works

Lithographic Patterning of Photoreactive Cell-Adhesive Proteins

Carrico, I. S., Maskarinec, S. A., Heilshorn, S. C., Mock, M. L., Liu, J. C., Nowatzki, P. J., Franck, C., Ravichandran, G., & Tirrell, D. A. (2007) · Journal of the American Chemical Society · doi:10.1021/ja070200b · Cited by 114 · see citing works

Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel

Jin, L., Feng, T., Shih, H. P., Zerda, R., Luo, A., Hsu, J., Mahdavi, A., Sander, M., Tirrell, D. A., Riggs, A. D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1301889110 · Cited by 108 · see citing works

Small peptide functionalized thiol–ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition

Gould, S. T., Darling, N. J., & Anseth, K. S. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.05.009 · Cited by 93 · see citing works

Islet Formation during the Neonatal Development in Mice

Miller, K. W. P., Kim, A., Kilimnik, G., Jo, J., Moka, U., Periwal, V., & Hara, M. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007739 · Cited by 75 · see citing works

Boundary crossing in epithelial wound healing

Fong, E. W. M., Tzlil, S., & Tirrell, D. A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1008291107 · Cited by 72 · see citing works

Basement Membrane in Pancreatic Islet Function

Kragl, M., & Lammert, E. (2010) · Advances in experimental medicine and biology · doi:10.1007/978-90-481-3271-3_10 · Cited by 58 · see citing works

Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling

Christensen, T., Trabbic-Carlson, K., Liu, W., & Chilkoti, A. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2006.09.020 · Cited by 52 · see citing works

Exocytosis of Insulin

Rozzo, A., Meneghel‐Rozzo, T., Delakorda, S. L., Yang, S., & Rupnik, M. S. (2009) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2008.04003.x · Cited by 48 · see citing works

Integrin‐linked kinase production prevents anoikis in human mesenchymal stem cells

Benoit, D. S. W., Tripodi, M. C., Blanchette, J. O., Langer, S. J., Leinwand, L. A., & Anseth, K. S. (2007) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31292 · Cited by 48 · see citing works

Collective Cell Migration on Artificial Extracellular Matrix Proteins Containing Full‐Length Fibronectin Domains

Fong, E. W. M., & Tirrell, D. A. (2010) · Advanced Materials · doi:10.1002/adma.201002448 · Cited by 36 · see citing works

The influence of extracellular matrix composition on the differentiation of neuronal subtypes in tissue engineered innervated intestinal smooth muscle sheets

Raghavan, S. A., & Bitar, K. N. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.05.037 · Cited by 35 · see citing works

In Vitro Multilineage Differentiation and Self-Renewal of Single Pancreatic Colony-Forming Cells from Adult C57Bl/6 Mice

Jin, L., Tao, F., Zerda, R., Chen, C., Riggs, A. D., & Ku, H. T. (2013) · Stem Cells and Development · doi:10.1089/scd.2013.0466 · Cited by 25 · see citing works

Colony-Forming Progenitor Cells in the Postnatal Mouse Liver and Pancreas Give Rise to Morphologically Distinct Insulin-Expressing Colonies in 3D Cultures

Jin, L., Feng, T., Chai, J., Ghazalli, N., Gao, D., Zerda, R., Li, Z., Hsu, J., Mahdavi, A., Tirrell, D. A., et al. (2014) · The Review of Diabetic Studies · doi:10.1900/rds.2014.11.35 · Cited by 9 · see citing works

Towards organoid culture without Matrigel

Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works

Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering

Perestrelo, A. R., Águas, A. C., Rainer, A., & Forte, G. (2015) · Sensors · doi:10.3390/s151229848 · Cited by 151 · see citing works

Insulin-positive, Glut2-low cells present within mouse pancreas exhibit lineage plasticity and are enriched within extra-islet endocrine cell clusters

Beamish, C. A., Strutt, B., Arany, E., & Hill, D. (2016) · Islets · doi:10.1080/19382014.2016.1162367 · Cited by 49 · see citing works

Cells with surface expression of CD133highCD71low are enriched for tripotent colony-forming progenitor cells in the adult murine pancreas

Jin, L., Gao, D., Feng, T., Tremblay, J. R., Ghazalli, N., Luo, A., Rawson, J., Quijano, J. C., Chai, J., Wedeken, L., et al. (2016) · Stem Cell Research · doi:10.1016/j.scr.2015.11.015 · Cited by 32 · see citing works

A matrigel-free method for culture of pancreatic endocrine-like cells in defined protein-based hydrogels

Kozlowski, M. T., Zook, H. N., Chigumba, D. N., Johnstone, C. P., Caldera, L. F., Shih, H.-P., Tirrell, D. A., & Ku, H. T. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1144209 · Cited by 20 · see citing works

Adult Murine Pancreatic Progenitors Require Epidermal Growth Factor and Nicotinamide for Self-Renewal and Differentiation in a Serum- and Conditioned Medium-Free Culture

Wedeken, L., Luo, A., Tremblay, J. R., Rawson, J., Jin, L., Gao, D., Quijano, J. C., & Ku, H. T. (2017) · Stem Cells and Development · doi:10.1089/scd.2016.0328 · Cited by 20 · see citing works

A GLIS3–CD133–WNT-signaling axis regulates the self-renewal of adult murine pancreatic progenitor-like cells in colonies and organoids

Tremblay, J. R., Lopez, K., & Ku, H. T. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.002818 · Cited by 8 · see citing works

Developments in gastrointestinal organoid cultures to recapitulate tissue environments

Kuhn, M. R., Wolcott, E. A., & Langer, E. M. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1521044 · Cited by 8 · see citing works

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Tremblay, J. R., LeBon, J. M., Luo, A., Quijano, J. C., Wedeken, L., Jou, K., Riggs, A. D., Tirrell, D. A., & Ku, H. T. (2016) · Journal of Visualized Experiments · doi:10.3791/54016 · Cited by 7 · see citing works

Glucocorticoid Signaling Enhances Expression of Glucose-Sensing Molecules in Immature Pancreatic Beta-Like Cells Derived from Murine Embryonic Stem Cells In Vitro

Ghazalli, N., Wu, X., Walker, S., Trieu, N., Hsin, L.-Y., Choe, J., Chen, C., Hsu, J., LeBon, J. M., Kozlowski, M. T., et al. (2018) · Stem Cells and Development · doi:10.1089/scd.2017.0160 · Cited by 6 · see citing works

Characterization and Differentiation of Sorted Human Fetal Pancreatic ALDH hi and ALDH hi /CD133 + Cells Toward Insulin-Expressing Cells

Oakie, A., Li, J., Fellows, G. F., Hess, D. A., & Wang, R. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0135 · Cited by 6 · see citing works

Revolutionizing digestive system tumor organoids research: Exploring the potential of tumor organoids

Xiu, Z., Yang, Q., Xie, F., Han, F., He, W., & Liao, W. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314241255470 · Cited by 5 · see citing works

Isolation and Characterization of Colony-Forming Progenitor Cells from Adult Pancreas

Quijano, J. C., Tremblay, J. R., Rawson, J., & Ku, H. T. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9631-5_6 · Cited by 4 · see citing works

Engineering Antigen-Specific Tolerance to an Artificial Protein Hydrogel

Rapp, P. B., Baccile, J. A., Galimidi, R. P., & Vielmetter, J. (2024) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.3c01430 · Cited by 3 · see citing works

Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems

Zook, H. N., Quijano, J. C., Ortiz, J., Donohue, C., Erdem, N., & Ku, H. T. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103847 · Cited by 2 · see citing works

Rare, Tightly-Bound, Multi-Cellular Clusters in the Pancreatic Ducts of Adult Mice Function Like Progenitor Cells and Survive and Proliferate After Acinar Cell Injury

Tremblay, J. R., Ortiz, J., Quijano, J. C., Zook, H. N., Erdem, N., LeBon, J. M., Li, W., Jou, K., Tsark, W. M. K., Mann, J. R., et al. (2024) · Stem Cells · doi:10.1093/stmcls/sxae005 · Cited by 2 · see citing works

Engraftment of Reprogrammed Human Bile Duct Cells Transiently Rescues Diabetes in Mice

Banga, A., Dutton, J., Mysz, M. A., Norris, B. J., Ogle, B., Tolar, J., Schumacher, R., & Flory, C. (2018) · Journal of Stem Cell Research & Therapy · doi:10.4172/2157-7633.1000432

Cells with surface expression of CD133highCD71low are enriched for tripotent colony-forming progenitor cells in the adult murine pancreas

Jin, L., Gao, D., Feng, T., Tremblay, J. R., Ghazalli, N., Luo, A., Rawson, J., Quijano, J. C., Chai, J., Wedeken, L., et al. (2016) · Stem Cell Research · doi:10.1016/j.scr.2015.11.015 · Cited by 32 · see citing works

Laminin-1 promotes differentiation of fetal mouse pancreatic beta-cells.

Jiang, F., Cram, D. S., DeAizpurua, H. J., & Harrison, L. C. (1999) · Diabetes · doi:10.2337/diabetes.48.4.722 · Cited by 149 · see citing works

Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells

Scavuzzo, M. A., Yang, D., & Borowiak, M. (2017) · Scientific Reports · doi:10.1038/s41598-017-11169-1 · Cited by 21 · see citing works

IMPAN Cells: A Pancreatic Model for Differentiation into Endocrine Cells

Klein, T., Frandsen, U., Heller, R. S., & Serup, P. (2001) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.2001.2579 · Cited by 2 · see citing works

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Tremblay, J. R., LeBon, J. M., Luo, A., Quijano, J. C., Wedeken, L., Jou, K., Riggs, A. D., Tirrell, D. A., & Ku, H. T. (2016) · Journal of Visualized Experiments · doi:10.3791/54016 · Cited by 7 · see citing works