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Leptin-Receptor-Expressing Mesenchymal Stromal Cells Represent the Main Source of Bone Formed by Adult Bone Marrow
Open accessAbstract
Studies of the identity and physiological function of mesenchymal stromal cells (MSCs) have been hampered by a lack of markers that permit both prospective identification and fate mapping in vivo. We found that Leptin Receptor (LepR) is a marker that highly enriches bone marrow MSCs. Approximately 0.3% of bone marrow cells were LepR(+), 10% of which were CFU-Fs, accounting for 94% of bone marrow CFU-Fs. LepR(+) cells formed bone, cartilage, and adipocytes in culture and upon transplantation in vivo. LepR(+) cells were Scf-GFP(+), Cxcl12-DsRed(high), and Nestin-GFP(low), markers which also highly enriched CFU-Fs, but negative for Nestin-CreER and NG2-CreER, markers which were unlikely to be found in CFU-Fs. Fate-mapping showed that LepR(+) cells arose postnatally and gave rise to most bone and adipocytes formed in adult bone marrow, including bone regenerated after irradiation or fracture. LepR(+) cells were quiescent, but they proliferated after injury. Therefore, LepR(+) cells are the major source of bone and adipocytes in adult bone marrow.
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“Type H vessels respond to the administration of intermittent anabolic PTH (iPTH), which promotes the detachment of leptin receptor-expressing (LepR+) cells, which are pericytes that can differentiate into osteoprogenitors,134 from CD31hi/Edmhi vessels.”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [134]
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Cites
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Leptin Receptor Expression in Mouse Intracranial Perivascular Cells
Yuan, X., Caron, A., Wu, H., & Gautron, L. (2018) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2018.00004 · Cited by 33
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BackgroundResultBone marrow adipose cells – cellular interactions and changes with obesity
Boroumand, P., & Klip, A. (2020) · Journal of Cell Science · doi:10.1242/jcs.238394 · Cited by 29
“…that a single population of MSCs, defined as CXCL12 abundant reticular (CAR)/LepR+/NestinGFPdim cells, are plentiful in various bone marrow niches (Zhou et al., 2014) and are an important contributor to adipocyte cellularity and communication with HSCs (Omatsu et al., 2010; Sugiyama et al., 2006).”
BackgroundThe Amino Acid Sensor Eif2ak4/GCN2 Is Required for Proliferation of Osteoblast Progenitors in Mice
Hu, G., Yu, Y., Tang, Y. J., Wu, C., Long, F., & Karner, C. M. (2020) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.4091 · Cited by 31
“SSPC are generally thought to be quiescent in vivo but rapidly proliferate in response to osteoinductive signals or injury.((2)) Con-”
BackgroundMarrow adipogenic lineage precursor: A new cellular component of marrow adipose tissue
Zhong, L., Yao, L., Seale, P., & Qin, L. (2021) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2021.101518 · Cited by 30
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“Interestingly, we identified two clusters of 300 genes similarly expressed in the Lepr and Nes cells and highly enriched in MSC signature genes (adipoq, lepr, cxcl12, cxcl14, gpx3, pdgfra, agt, grem1 igfbp5, foxc1, cdh2) (Worthley et al., 2015; Zhou et al., 2014; Zhao et al., 2019), which contains candidatemarkers to…”
BackgroundEndo, T., Takahata, M., Koike, Y., Fujita, R., Suzuki, R., Hisada, Y., Hasegawa, Y., Suzuki, H., Yamada, K., Iwata, A., et al. (2022) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-021-01292-5 · Cited by 32
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TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities
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“Further, these cells overlap with Lepr-cremarked cells in adult bonemarrow;((7)) therefore, these cells are also”
BackgroundHypertrophic chondrocytes at the junction of musculoskeletal structures
Chen, N., Wu, R. W. H., Lam, Y., Chan, W. C. W., & Chan, D. T.-M. (2023) · Bone Reports · doi:10.1016/j.bonr.2023.101698 · Cited by 34
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Bone marrow niches for hematopoietic stem cells
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“57,68 Terminally differentiated osteoblasts are confined to the inner surfaces of bone en-closure and are therefore directly exposed to hematopoietic cells residing in endosteal regions.”
BackgroundIdentity of Gli1+ cells in the bone marrow
Sena, I. F. G., Prazeres, P. H. D. M., Santos, G. S. P., Borges, I. T., de Azevedo, P. O., Andreotti, J. P., de Almeida, V. M., Paiva, A. E., Guerra, D. A. P., Lousado, L., et al. (2017) · Experimental Hematology · doi:10.1016/j.exphem.2017.06.349 · Cited by 30
“Additionally, several other stromal cell types have been described in the bone marrow: NG2+, PDGFRα+, Gremlin-1+, Prx1+, LepR+, and Nestin-GFP+ cells [13].”
Bone Marrow Niches for Skeletal Progenitor Cells and their Inhabitants in Health and Disease
Herrmann, M., & Jakob, F. J. (2019) · Current Stem Cell Research & Therapy · doi:10.2174/1574888x14666190123161447 · Cited by 28
A bone marrow niche-derived molecular switch between osteogenesis and hematopoiesisPreprint
Galán-Díez, M., & Kousteni, S. (2018) · Genes & Development · doi:10.1101/gad.314013.118 · Cited by 30
“…ISSN 0890-9369/18; www.genesdev.org high levels of CXCL12 and SCF (CXCL12-abundant reticular [CAR] cells)makeup themajority of perivascular cells (Omatsu et al. 2010) and overlap with perisinusoidal cells, identified on the basis of their expression of the leptin receptor (LepR) (Zhou et al. 2014).”
BackgroundLoss of KDM4B impairs osteogenic differentiation of OMSCs and promotes oral bone aging
Deng, P., Chang, I., Wang, J., Badreldin, A. A., Li, X., Yu, B., & Wang, C. (2022) · International Journal of Oral Science · doi:10.1038/s41368-022-00175-3 · Cited by 32
“Previous studies report that MSCs from long bones could differentiate into both osteoblasts and adipocytes, but the lineage commitment to each cell type is mutually exclusive.(24,25) Thus, we explored whether loss of bone formation in aged Kdm4b and Kdm4b mice led to fat accumulation in the marrow space of the mandibl…”
Chen, S., Lin, Y., Yang, H., Li, Z., Li, S., Chen, D., Hao, W., Zhang, S., Chao, H., Zhang, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-56112-5 · Cited by 29
Chen, K. G., Johnson, K. R., McKay, R. D. G., & Robey, P. G. (2017) · Stem Cells · doi:10.1002/stem.2711 · Cited by 29
Bone Marrow Adipocytes: A Link between Obesity and Bone Cancer
Reagan, M. R., Fairfield, H. E., & Rosen, C. J. (2021) · Cancers · doi:10.3390/cancers13030364 · Cited by 28
Exercise to Mend Aged-tissue Crosstalk in Bone Targeting Osteoporosis & Osteoarthritis
Little, S. E., Rubin, J. E., Diekman, B. O., Rubin, C. T., McGrath, C., Pagnotti, G. M., Klett, E. L., & Styner, M. (2021) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2021.08.011 · Cited by 30
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“…in the context of osteogenic differentiation of dental stem cells is rare, it was shown that mesenchymal stromal cells that express leptin receptors not only give rise to most of the new formed bone in adult bone marrow but are also the ones responsible for regeneration after a trauma [60].”
BackgroundCell-by-Cell Deconstruction of Stem Cell Niches
Tikhonova, A. N., Lasry, A., Austin, R. J., & Aifantis, I. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.06.013 · Cited by 34
“Taking advantage of transgenic labeling of Leptin-receptor-expressing (Lepr+) mesenchymal populations, enriched for MSC activity (Yue et al., 2016; Zhou et al., 2014), our group identified two transcriptionally similar but distinct sub-populations of quiescent MSPC: adipogenesisand osteogenesis-primed cells.”
BackgroundBuilding and maintaining joints by exquisite local control of cell fate
Smeeton, J., Askary, A., & Crump, J. G. (2016) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.245 · Cited by 28
Ally to adversary: mesenchymal stem cells and their transformation in leukaemia
Sharma, M., Ross, C. R., & Srivastava, S. (2019) · Cancer Cell International · doi:10.1186/s12935-019-0855-5 · Cited by 29
Recent Advances in Single-Cell View of Mesenchymal Stem Cell in Osteogenesis
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“The expression of adult MSC marker Lepr in 16-month-old EMPs was significantly higher than that of 1 month, which was consistent with the previously observations that Lepr-Cre labeled MSCs only appeared in the bone marrow of adult mice, but not in young mice (Zhou et al., 2014; Zhong et al., 2020).”
BackgroundResultInfluentialDiversity in the bone marrow niche: Classic and novel strategies to uncover niche composition
Sánchez‐Lanzas, R., Kalampalika, F., & Ganuza, M. (2022) · British Journal of Haematology · doi:10.1111/bjh.18355 · Cited by 28
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“which regulate HSC function, including nonmyelinating Schwann cells [4], Nestinþ mesenchymal stem cells (MSCs) [5], leptin receptor-expressing (LepRþ) cells [6], and cells expressing stem cell factor (Scf) [7].”
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“This question could be clarified in future studies by using the MSC cell-tracking reporter murine models using nestin [10] or leptin receptor [20] promoters.”
Perivascular osteoprogenitors are associated with transcortical channels of long bones
Root, S. H., Wee, N. K. Y., Novak, S., Rosen, C. J., Baron, R., Matthews, B. G., & Kalajzić, I. (2020) · Stem Cells · doi:10.1002/stem.3159 · Cited by 27
“Although generated postnatally, these cells are quiescent in nature until bone injury occurs which triggers their proliferation and differentiation.(4) Dentin matrix protein 1 (DMP1) is a member of the SIBLING fam-”
BackgroundInferring Gene Networks in Bone Marrow Hematopoietic Stem Cell-Supporting Stromal Niche Populations
Desterke, C., Petit, L., Sella, N., Chevallier, N., Cabeli, V., Coquelin, L., Durand, C., Oostendorp, R. A. J., Isambert, H., Jaffredo, T., et al. (2020) · iScience · doi:10.1016/j.isci.2020.101222 · Cited by 27
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Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure
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“Leptin, a well-studied protein, has direct anabolic effect on osteoblast (55), and BMSCs rich in leptin receptor (LepR) are the main source of bone formation (56).”
BackgroundPeriosteal Skeletal Stem Cells and Their Response to Bone Injury
Zhang, N., Hu, L., Cao, Z., Liu, X., & Pan, J. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.812094 · Cited by 27
“BM, however, appears to be a much larger pool of cells (Méndez-Ferrer et al., 2010; Zhou et al., 2014).”
BackgroundBone repair with skeletal stem cells: rationale, progress to date and clinical application
Jones, E., Giannoudis, P. V., & Kouroupis, D. (2016) · Therapeutic Advances in Musculoskeletal Disease · doi:10.1177/1759720x16642372 · Cited by 27
“2014], Zhou and colleagues [Zhou et al. 2014] and Chan and colleagues [Chan et al.”
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“reported that iPTH controls the fate of cells expressing Prx1, an early MSC marker expressed in the lineage of all LepR cells.((19)) Indeed, iPTH promotes the commitment of LepR cells toward the osteoblast lineage at the expense of adipocyte differentiation, via their PTH/PTHrP receptor.”
Zou, N.-Y., Liu, R., & Li, C. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.818462 · Cited by 25
“Moreover, a subpopulation of CTSK-mGFP cells also expressed Gremlin1 and Nestin, but the CTSK-mGFP cells did not display markers of hematopoietic mesenchymal cells, such as LEPR, CD146, and CD140α (Sacchetti et al., 2007; Morikawa et al., 2009; Pinho et al., 2013; Zhou et al., 2014; Debnath et al., 2018).”
BackgroundAdvancing skeletal health and disease research with single-cell RNA sequencing
Lin, P., Gan, Y., He, J., Lin, S., Xu, J., Chang, L., Zhao, L., Zhu, J. J., Zhang, L., Huang, S., et al. (2024) · Military Medical Research · doi:10.1186/s40779-024-00538-3 · Cited by 26
“In 2014, Zhou et al. [182] uncovered LepR + skeletal stem cells (SSCs) in the bone marrow.”
BackgroundPeriodontal Ligament Cell Apoptosis Activates Lepr+ Osteoprogenitors in Orthodontics
Liu, H., Zhang, Y., Zhang, Y., Huang, Y., Yang, Y., Zhao, Y., Chen, S., Deng, J., Li, W., & Han, B. (2024) · Journal of Dental Research · doi:10.1177/00220345241262706 · Cited by 25
“…Previous studies have reported that Lepr + osteoprogenitor cells account for approximately 94% of the stem cell population in adult long bones (Zhou et al. 2014), serving as an important source of osteoblasts that rapidly respond to stress conditions, such as fractures, radiation damage, and…”
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Endodontic Regeneration Therapy: Current Strategies and Tissue Engineering Solutions
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“However, the lack of reliable cell surface markers, standardized tissue dissociation protocols, and functional stem cell assays has hindered the identification of presumptive stem cells within the human skeleton [112–116].”
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“Recently, LepR has been fully discovered as a potential marker of bone marrow mesenchymal stromal cells andperiosteum-derived stem/progenitor cells [16,17].”
BackgroundSelf-citationHuman Cell Atlas and cell-type authentication for regenerative medicine
Panina, Y., Karagiannis, P., Kurtz, A., Stacey, G. N., & Fujibuchi, W. (2020) · Experimental & Molecular Medicine · doi:10.1038/s12276-020-0421-1 · Cited by 27
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The RIG-I–NRF2 axis regulates the mesenchymal stromal niche for bone marrow transplantation
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Deep learning and genome-wide association meta-analyses of bone marrow adiposity in the UK Biobank
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“Across all three of these tissues (subcutaneous adipose tissue, visceral-omentum adipose tissue and skeletal muscle), TWAS identi fi ed 31, 49, 32, and 64 genes that, after Bonferroni correction, were signi fi cantly associated with BMFF of the femoral head (Supplementary Data 26; Supplementary Fig.”
InfluentialChen, K. G., Johnson, K. R., & Robey, P. G. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.09.014 · Cited by 25
“As already discussed above, Nes-GFP and NesCreER have different transgene expression patterns in the BM (Isern et al., 2014; Ono et al., 2014; Zhou et al., 2014), which may be partially explained by their differences in regulatory elements and their orientations in the expression vectors (Figure 2A).”
BackgroundMethodInfluentialLeukocyte Trafficking and Regulation of Murine Hematopoietic Stem Cells and Their Niches
Lucas, D. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.00387 · Cited by 25
“, can differentiate to osteoblasts, adipocytes, and chondrocytes) in vitro, and upon transplantation in ossicles can generate an ectopic niche that supports extramedullary hematopoiesis (16, 17).”
BackgroundKLF2+ stemness maintains human mesenchymal stem cells in bone regeneration
Zhou, Y., Liu, C., He, J., Dong, L., Zhu, H., Zhang, B., Feng, X., Weng, W., Cheng, K., Yu, M., et al. (2019) · Stem Cells · doi:10.1002/stem.3120 · Cited by 25
The Novel Role of PGC1α in Bone Metabolism
Buccoliero, C., Dicarlo, M., Pignataro, P., Gaccione, F., Colucci, S. C., Colaianni, G., & Grano, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094670 · Cited by 25
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Yan, Q., Gao, H., Yao, Q., Ling, K., & Xiao, G. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.101639 · Cited by 26
“Osteoblasts are differentiated from the mesenchymal stem cells (MSCs) (14, 15).”
BackgroundPerivascular Mesenchymal Stem/Stromal Cells, an Immune Privileged Niche for Viruses?
Lebeau, G., Ah-Pine, F., Daniel, M., Bedoui, Y., Vagner, D., Frumence, É., & Gasque, P. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23148038 · Cited by 25
Niu, Y., Xiao, H., Wang, B., Wang, Z., Du, K., Wang, Y., & Wang, L. (2023) · Pharmaceutical Biology · doi:10.1080/13880209.2023.2207592 · Cited by 25
“In addition, osteopontin is involved in the maintenance of the HSPCs compartment and the protection of HSPCs from myelosuppressive stress (Zhang et al. 2003; Zhou et al. 2014).”
BackgroundInfluentialТодосенко, Н. М., Хазиахматова, О. Г., Malashchenko, V. V., Yurova, K. A., Bograya, M., Beletskaya, M., Vulf, M. A., Mikhailova, L. V., Minchenko, A., Soroko, I., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241512259 · Cited by 28
“Effects of BMAs on BM-MSCs: BMA expansion is accompanied by the release of pro-inflammatory mediators that have a damaging effect on neighboring cells, induce BM-MSC senescence (via ROS), and decrease the functional properties/number of stem cells [56,57,71].”
BackgroundInfluentialIdentification of an intronic enhancer regulating RANKL expression in osteocytic cells
Yan, M., Tsukasaki, M., Muro, R., Ando, Y., Nakamura, K., Komatsu, N., Nitta, T., Okamura, T., Okamoto, K., & Takayanagi, H. (2023) · Bone Research · doi:10.1038/s41413-023-00277-6 · Cited by 26
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Regulation of hematopoietic stem cells in the niche
Zhao, M. L., & Li, L. (2015) · Science China Life Sciences · doi:10.1007/s11427-015-4960-y · Cited by 27
Ouchi, T., Morikawa, S., Shibata, S., Fukuda, K., Okuno, H., Fujimura, T., Kuroda, T., Ohyama, M., Akamatsu, W., Nakagawa, T., et al. (2016) · Differentiation · doi:10.1016/j.diff.2016.04.003 · Cited by 25
Concise Review: The Malignant Hematopoietic Stem Cell Niche
Yao, J.-C., & Link, D. C. (2016) · Stem Cells · doi:10.1002/stem.2487 · Cited by 26
Imaging methods used to study mouse and human HSC niches: Current and emerging technologies
Tjin, G., Figueroa, E. F., Duarte, D., Straszkowski, L., Scott, M., Khorshed, R., Purton, L. E., & Celso, C. L. (2018) · Bone · doi:10.1016/j.bone.2018.04.022 · Cited by 23
“[9,11], the latter which have been shown to be the source of all adipocytes in BM [9].”
BackgroundHayes, A. J., Smith, S. M., Caterson, B., & Melrose, J. (2018) · Stem Cells · doi:10.1002/stem.2860 · Cited by 25
Bioengineering Hematopoietic Stem Cell Niche toward Regenerative Medicine
Sugimura, R. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.10.010 · Cited by 25
Single-cell and spatial transcriptomics approaches of the bone marrow microenvironment
Al-Sabah, J., Baccin, C., & Haas, S. F. (2019) · Current Opinion in Oncology · doi:10.1097/cco.0000000000000602 · Cited by 28
“Adipo-CAR cells associate preferentially with sinusoidal vasculature, in line with their similarity to Lepr-Cre and their described localization to sinusoids [9].”
BackgroundInfluentialIntervertebral Disc and Adipokine Leptin—Loves Me, Loves Me Not
Ćurić, G. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms22010375 · Cited by 24
“BM of adult mice harbors LepR positive multipotent mesenchymal stromal cells, which are the major sources of bone cells and adipocytes, as well as cartilage during bone healing in vivo [90].”
BackgroundA cross-species analysis of systemic mediators of repair and complex tissue regeneration
Losner, J., Courtemanche, K., & Whited, J. L. (2021) · npj Regenerative Medicine · doi:10.1038/s41536-021-00130-6 · Cited by 26
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“…also termed Prx1), CXC motif ligand 12 (CXCL12) and Gremlin 1 (Grem1)- expressing cells give rise to terminally differentiated osteocytes, chondrocytes, adipocytes and fibroblasts (Greenbaum et al., 2013; Kramann et al., 2015; Omatsu et al., 2010; Worthley et al., 2015; Zhou et al., 2014).”
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Sirt1 Promotes a Thermogenic Gene Program in Bone Marrow Adipocytes: From Mice to (Wo)Men
Artsi, H., Gurt, I., El-Haj, M., Müller, R., Kuhn, G. A., Ben-Shalom, G., Cohen‐Kfir, E., Abramowitz, E., Kandel, L., Safran, O., et al. (2019) · Frontiers in Endocrinology · doi:10.3389/fendo.2019.00126 · Cited by 25
“BMSCs that express the leptin receptor (LepR) have the capacity to differentiate into both adipocytes and osteoblasts, while LepR is not expressed by neither mature osteoblasts nor marrow adipocytes, suggesting that LepR in BMSCs influences lineage allocation (17).”
BackgroundGleitz, H. F., Kramann, R., & Schneider, R. K. (2018) · The Journal of Pathology · doi:10.1002/path.5078 · Cited by 23
“Nes-MSCs express the classic mesenchymal-specific markers platelet-derived growth factor receptor-α (PDGFRA/CD140a), CD51, and Sca-1 [30,31], as well as LepR [32] and the pericyte marker neuron/glial antigen 2 (NG2) [33].”
BackgroundTissue-resident glial cells associate with tumoral vasculature and promote cancer progression
Rocha, B. G. S., Picoli, C. C., Gonçalves, B. Ô. P., da Silva, W. N., Costa, A. C., Moraes, M. M., Costa, P. A. C., Santos, G. S. P., Almeida, M. R., Silva, L. M., et al. (2022) · Angiogenesis · doi:10.1007/s10456-022-09858-1 · Cited by 24
The role of heme oxygenase-1 in hematopoietic system and its microenvironment
Szade, A., Szade, K., Mahdi, M., & Józkowicz, A. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03803-z · Cited by 25
“CAR phenotype highly overlaps with the later-described leptin receptor-expressing MSC (LepR +) [14].”
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Self-citationSkeletal stem cells: origins, definitions, and functions in bone development and disease
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“As LepR+ cells only emerge postnatally, they are not involved in the prenatal developmental or early postnatal generation of osteoblasts, but their contribution to the osteoblast pool increases with increasing age [19].”
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Targeting Osteogenesis-Angiogenesis Coupling for Bone Repair
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“We first characterized the changes in cell clusters from bone callus during fracture healing [7, 8, 10, 38, 39].”
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“Interestingly, Prx1Cre:Pgc1a fl/fl and LepRCre:Pgc-1a fl/fl mice, and Thy -/- mice showed higher bone adiposity and lower bone formation during OVX-induced osteoporosis, suggesting that Pgc1a and Thy1 play a role in regulating lineage allocation between BMAT and osteoblasts [39, 51].”
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“Transplantation of bone marrow mesenchymal stem cells (MSCs) may promote the regeneration of the injured uterus and abnormal endometrium (8-10).”
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Multipurposing CARs: Same engine, different vehicles
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Reduced Bone Mass in Collagen Prolyl 4‐Hydroxylase P4ha1 +/− ; P4ha2 −/− Compound Mutant Mice
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Impact of Environmental and Epigenetic Changes on Mesenchymal Stem Cells during Aging
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“Still, both of these populations were found to express Leptin Receptor and Gremlin 1, showing that these populations are comparable to previously reported SSC populations, and this also supported our microarray findings.”
BackgroundInfluential
Metadata
- Authors
- Zhou, B. O., Yue, R., Murphy, M. M., Peyer, J. G., & Morrison, S. J.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Morrison, Sean J.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2014-08
- Volume
- 15
- Issue
- 2
- Pages
- 154-168
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC4127103
- OpenAlex
- W2078719918
- Cited by
- 1,381
- References
- 53
- Citations by year
- 2026: 84 · 2025: 92 · 2024: 133 · 2023: 153 · 2022: 148 · 2021: 147 · 2020: 153 · 2019: 114 · 2018: 99 · 2017: 87 · 2016: 89 · 2015: 66 · 2014: 12
- FWCI
- 42.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Heart, Lung, and Blood Institute (HL097760, R01HL097760)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- mesenchymal stem cells, leptin receptor, bone marrow, bone formation, adipogenesis, chondrogenesis, CFU-F, fate mapping, cell markers, fracture healing, quiescent cells, stem cell proliferation
- MeSH terms
- Animals, Bone and Bones, Bone Marrow, Bone Marrow Cells, Flow Cytometry, Male, Mice, Inbred C57BL, Osteoblasts, Osteogenesis, Regeneration, Stem Cells, Tamoxifen, Adipocytes, Transgenes, Chondrocytes, Leptin, Cell Proliferation, Green Fluorescent Proteins, Mice, Receptors, Leptin, Mesenchymal Stem Cells
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