The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis
Open accessAbstract
Spermatogenesis generates mature male gametes and is critical for the proper transmission of genetic information between generations. However, the developmental landscapes of human spermatogenesis remain unknown. Here, we performed single-cell RNA sequencing (scRNA-seq) analysis for 2,854 testicular cells from donors with normal spermatogenesis and 174 testicular cells from one nonobstructive azoospermia (NOA) donor. A hierarchical model was established, which was characterized by the sequential and stepwise development of three spermatogonia subtypes, seven spermatocyte subtypes, and four spermatid subtypes. Further analysis identified several stage-specific marker genes of human germ cells, such as HMGA1, PIWIL4, TEX29, SCML1, and CCDC112. Moreover, we identified altered gene expression patterns in the testicular somatic cells of one NOA patient via scRNA-seq analysis, paving the way for further diagnosis of male infertility. Our work allows for the reconstruction of transcriptional programs inherent to sequential cell fate transition during human spermatogenesis and has implications for deciphering male-related reproductive disorders.
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Cites
HTSeq—a Python framework to work with high-throughput sequencing data
Anders, S., Pyl, P. T., & Huber, W. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btu638 · Cited by 21,464
Trapnell, C., Cacchiarelli, D., Grimsby, J., Pokharel, P., Li, S., Morse, M., Lennon, N. J., Livak, K. J., Mikkelsen, T. S., & Rinn, J. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2859 · Cited by 7,314
Spatial reconstruction of single-cell gene expression data
Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3192 · Cited by 7,055
Cited by
Construction of a human cell landscape at single-cell level
Han, X., Zhou, Z., Fei, L., Sun, H., Wang, R., Chen, Y., Chen, H., Wang, J., Tang, H., Ge, W., et al. (2020) · Nature · doi:10.1038/s41586-020-2157-4 · Cited by 1,087
Self-citationThe adult human testis transcriptional cell atlas
Guo, J., Grow, E. J., Mlcochova, H., Maher, G. J., Lindskog, C., Nie, X., Guo, Y., Takei, Y., Yun, J., Cai, L., et al. (2018) · Cell Research · doi:10.1038/s41422-018-0099-2 · Cited by 710
Zhao, L., Yao, C., Xing, X., Jing, T., Li, P., Zhu, Z., Yang, C., Zhai, J., Tian, R., Chen, H., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19414-4 · Cited by 298
Self-citationHuang, Y., Li, L., An, G., Yang, X., Cui, M., Song, X., Lin, J., Zhang, X., Yao, Z., Wan, C., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01232-7 · Cited by 59
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Metadata
- Authors
- Wang, M., Liu, X., Chang, G., Chen, Y., An, G., Yan, L., Gao, S., Xu, Y., Cui, Y., Dong, J., Chen, Y., Fan, X., Hu, Y., Song, K., Zhu, X., Gao, Y., Yao, Z., Bian, S., Hou, Y., Lu, J., Wang, R., Fan, Y., Lian, Y., Tang, W., Wang, Y., Liu, J., Zhao, L., Wang, L., Liu, Z., Yuan, R., Shi, Y., Hu, B., Ren, X., Tang, F., Zhao, X.-Y., & Qiao, J.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2018-10
- Volume
- 23
- Issue
- 4
- Pages
- 599-614.e4
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2889446142
- Cited by
- 475
- References
- 3
- FWCI
- 33.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wang, M., Liu, X., Chang, G., Chen, Y., An, G., Yan, L., Gao, S., Xu, Y., Cui, Y., Dong, J., Chen, Y., Fan, X., Hu, Y., Song, K., Zhu, X., Gao, Y., Yao, Z., Bian, S., Hou, Y., . . . Qiao, J. (2018). Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis. Cell Stem Cell. https://doi.org/10.1016/j.stem.2018.08.007
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