The DOI matches its Crossref record; 137 of the 137 references it cites were found in a registry or an index.
Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors
Open accessAbstract
Down syndrome (DS) is a genetic disorder driven by the triplication of chromosome 21 (T21) and characterized by a wide range of neurodevelopmental and physical disabilities. Transcriptomic analysis of tissue samples from individuals with DS has revealed that T21 induces a genome-wide transcriptional disruption. However, the consequences of T21 on the nuclear architecture and its interplay with the transcriptome remain unknown. In this study, we find that unlike human induced pluripotent stem cells (iPSCs), iPSC-derived neural progenitor cells (NPCs) exhibit genome-wide "chromosomal introversion," disruption of lamina-associated domains, and global chromatin accessibility changes in response to T21, consistent with the transcriptional and nuclear architecture changes characteristic of senescent cells. Treatment of T21-harboring NPCs with senolytic drugs alleviates the transcriptional, molecular, and cellular dysfunctions associated with DS. Our findings provide a mechanistic link between T21 and global transcriptional disruption and indicate that senescence-associated phenotypes may play a key role in the neurodevelopmental pathogenesis of DS.
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“fundamental aging processes can operate even before conception, for example, in the context of aged oocytes linked to Down syndrome12,13.”
From Cellular senescence and senolytics: the path to the clinic (2022) · cites this paper as [13]
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In the citing paper
“D + Q alleviated transcriptional changes and cellular dysfunction in an in vitro human neural cell Down syndrome model.”
From Cellular senescence and senolytics: the path to the clinic (2022) · cites this paper as [13]
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In the citing paper
“A recent study implicated cellular senescence and the beneficial effects of senolytics for Down syndrome (trisomy 21)13.”
From Cellular senescence and senolytics: the path to the clinic (2022) · cites this paper as [13]
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In the citing paper
“A third copy of chromosome 21 in neural progenitor cells leads to transcriptional and nuclear organizational changes similar to those in senescent cells.”
From Cellular senescence and senolytics: the path to the clinic (2022) · cites this paper as [13]
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In the citing paper
“These findings underscore a global epigenetic rewiring beyond localized chromosomal effects, aligning with prior observations of genomewide Ts21 chromatin reorganization (24-26).”
From Molecular and cellular processes disrupted in the early postnatal Down syndrome prefrontal cortex (2026) · cites this paper as [24]
In the citing paper
“the disruption of 3D genome organization has been demonstrated in neurodevelopmental and neurological neurodegenerative disorders such as Huntington’s disease, Fragile X syndrome, Down syndrome, etc.”
From Neuronal DNA double-strand breaks lead to genome structural variations and 3D genome disruption in neurodegeneration (2023) · cites this paper as [42]
In the citing paper
“Additionally, studies have shown senescence-associated 3D genome reorganization in dividing cell types42,140–143.”
From Neuronal DNA double-strand breaks lead to genome structural variations and 3D genome disruption in neurodegeneration (2023) · cites this paper as [42]
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A 3D Map of the Human Genome at Kilobase Resolution Reveals Principles of Chromatin Looping
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Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome
Lieberman-Aiden, E., van Berkum, N. L., Williams, L., Imakaev, M., Ragoczy, T., Telling, A., Amit, I., Lajoie, B. R., Sabo, P. J., Dorschner, M. O., et al. (2009) · Science · doi:10.1126/science.1181369 · Cited by 8,898
The serial cultivation of human diploid cell strains
Hayflick, L., & Moorhead, P. S. (1961) · Experimental Cell Research · doi:10.1016/0014-4827(61)90192-6 · Cited by 8,508
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Items 101–104 of 104
Aberrant chromatin remodeling influences human neural cell fate change in Trisomy 21Preprint
Klein, J. A., Upadhya, S., Nathanson, A. J., Silvian, D., Zaniewski, E. F., Morris, R., Haas, W., & Barrett, L. E. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.01.729170
Baxter, L. L., Lee, S., Fuentes, K., Mosley, I. A., Raymond, J. D., Guedj, F., Zhou, D., Slonim, D. K., Glotfelty, E. J., Tweedie, D., et al. (2026) · Biology Open · doi:10.1242/bio.062807
Developmental senescence misregulation in congenital disorders: CHARGE syndrome as a candidate model
Arana, Á. J., Palau-Irisarri, P., León, Y., Silva‐Álvarez, S. D., Collado, M., Sánchez, L., & Magariños, M. (2026) · Aging · doi:10.18632/aging.206396
Disrupted chromatin architecture blocks B lymphopoiesis in Down syndromePreprint
Liao, Z., Zhang, M., Soong, B. Y., Zhu, B., Boshans, L. L., Wang, K., Quijada‐Álamo, M., Osakwe, I., Wang, Z., Fiore, P., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.10.07.757425
Metadata
- Authors
- Meharena, H. S., Marco, A., Dileep, V., Lockshin, E. R., Akatsu, G. Y., Mullahoo, J., Watson, L. A., Ko, T., Guerin, L. N., Abdurrob, F., Rengarajan, S., Papanastasiou, M., Jaffe, J. D., & Tsai, L.-H.
- Affiliations
- Massachusetts Institute of Technology (US)
- Broad Institute (US)
- Corresponding author
- Meharena, Hiruy S.
- Tsai, Li-Huei
- ORCID
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2022-01
- Volume
- 29
- Issue
- 1
- Pages
- 116-130.e7
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC8805993
- OpenAlex
- W4205519160
- Cited by
- 92
- References
- 137
- Citations by year
- 2026: 14 · 2025: 19 · 2024: 19 · 2023: 29 · 2022: 11
- FWCI
- 19.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell stem cell (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-nd)
- Funders
- National Institutes of Health (3U54HG008097-05S1)
- Burroughs Wellcome Fund
- Massachusetts Institute of Technology
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Down syndrome and intellectual disability research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Down syndrome, cellular senescence, neural progenitor cells, nuclear organization, lamina-associated domains, chromatin accessibility, senolytics, iPSC-derived neural progenitor cells
- MeSH terms
- Down Syndrome, Humans, Gene Expression Profiling, Induced Pluripotent Stem Cells, Neural Stem Cells, Transcriptome
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