The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Platform for Induction and Maintenance of Transgene-free hiPSCs Resembling Ground State Pluripotent Stem Cells
Open accessAbstract
Cell banking, disease modeling, and cell therapy applications have placed increasing demands on hiPSC technology. Specifically, the high-throughput derivation of footprint-free hiPSCs and their expansion in systems that allow scaled production remains technically challenging. Here, we describe a platform for the rapid, parallel generation, selection, and expansion of hiPSCs using small molecule pathway inhibitors in stage-specific media compositions. The platform supported efficient and expedited episomal reprogramming using just OCT4/SOX2/SV40LT combination (0.5%-4.0%, between days 12 and 16) in a completely feeder-free environment. The resulting hiPSCs are transgene-free, readily cultured, and expanded as single cells while maintaining a homogeneous and genomically stable pluripotent population. hiPSCs generated or maintained in the media compositions described exhibit properties associated with the ground state of pluripotency. The simplicity and robustness of the system allow for the high-throughput generation and rapid expansion of a uniform hiPSC product that is applicable to industrial and clinical-grade use.
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Cites
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
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A ROCK inhibitor permits survival of dissociated human embryonic stem cells
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Deterministic direct reprogramming of somatic cells to pluripotency
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Cited by
CAR-NK cells: A promising cellular immunotherapy for cancer
Xie, G., Dong, H., Liang, Y., Ham, J. D., Rizwan, R., & Chen, J. (2020) · eBioMedicine · doi:10.1016/j.ebiom.2020.102975 · Cited by 800
“[41] Compared to differentiated NK cells, iPSCs can be more efficiently engineered to stably express a CAR.”
BackgroundDe Los Angeles, A., Ferrari, F., Xi, R., Fujiwara, Y., Benvenisty, N., Deng, H., Hochedlinger, K., Jaenisch, R., Lee, S., Leitch, H. G., et al. (2015) · Nature · doi:10.1038/nature15515 · Cited by 396
Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State
Gu, W., Gaeta, X., Sahakyan, A., Chan, A. B., Hong, C. S., Kim, R., Braas, D., Plath, K., Lowry, W. E., & Christofk, H. R. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.08.008 · Cited by 283
Genome engineering of induced pluripotent stem cells to manufacture natural killer cell therapies
Shankar, K., Capitini, C. M., & Saha, K. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-01741-4 · Cited by 85
Single‐cell pluripotency regulatory networks
Stumpf, P. S., Ewing, R., & MacArthur, B. D. (2016) · PROTEOMICS · doi:10.1002/pmic.201500528 · Cited by 4
Metadata
- Authors
- Valamehr, B., Robinson, M., Abujarour, R., Rezner, B., Vranceanu, F., Le, T., Medcalf, A., Lee, T. T., Fitch, M., Robbins, D., & Flynn, P.
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-03
- Volume
- 2
- Issue
- 3
- Pages
- 366-381
- ISSN
- 2213-6711 (ISSN-L)
- OpenAlex
- W2062504594
- Cited by
- 180
- References
- 9
- FWCI
- 7.91
- Type
- journal article
- Language
- en
- Open access
- gold
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- Valamehr, B., Robinson, M., Abujarour, R., Rezner, B., Vranceanu, F., Le, T., Medcalf, A., Lee, T. T., Fitch, M., Robbins, D., & Flynn, P. (2014). Platform for Induction and Maintenance of Transgene-free hiPSCs Resembling Ground State Pluripotent Stem Cells. Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2014.01.014
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