Acellular Lung Scaffolds Direct Differentiation of Endoderm to Functional Airway Epithelial Cells: Requirement of Matrix-Bound HS Proteoglycans
Open accessAbstract
Efficient differentiation of pluripotent cells to proximal and distal lung epithelial cell populations remains a challenging task. The 3D extracellular matrix (ECM) scaffold is a key component that regulates the interaction of secreted factors with cells during development by often binding to and limiting their diffusion within local gradients. Here we examined the role of the lung ECM in differentiation of pluripotent cells in vitro and demonstrate the robust inductive capacity of the native lung matrix alone. Extended culture of stem cell-derived definitive endoderm on decellularized lung scaffolds in defined, serum-free medium resulted in differentiation into mature airway epithelia, complete with ciliated cells, club cells, and basal cells with morphological and functional similarities to native airways. Heparitinase I, but not chondroitinase ABC, treatment of scaffolds revealed that the differentiation achieved is dependent on heparan sulfate proteoglycans and its bound factors remaining on decellularized scaffolds.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Surface proteoglycans that are necessary for successful organoid formation may be removed by harsh decellularization.
“Surface proteoglycans that are necessary for successful organoid formation may be removed by harsh decellularization121.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [121]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442 · see citing works
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
Discher, D. E., Mooney, D. J., & Zandstra, P. W. (2009) · Science · doi:10.1126/science.1171643 · Cited by 2,666 · see citing works
Murry, C., & Keller, G. (2008) · Cell · doi:10.1016/j.cell.2008.02.008 · Cited by 1,805 · see citing works
Basal cells as stem cells of the mouse trachea and human airway epithelium
Rock, J. R., Onaitis, M. W., Rawlins, E. L., Lu, Y., Clark, C. P., Xue, Y., Randell, S. H., & Hogan, B. L. M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906850106 · Cited by 1,568 · see citing works
Kimura, S., Hara, Y., Pineau, T., Fernández‐Salguero, P. M., Fox, C. H., Ward, J. M., & Gonzalez, F. J. (1996) · Genes & Development · doi:10.1101/gad.10.1.60 · Cited by 1,221 · see citing works
Tissue-Engineered Lungs for in Vivo Implantation
Petersen, T. H., Calle, E. A., Zhao, L., Lee, E. J., Gui, L., Raredon, M. S. B., Gavrilov, K., Yi, T., Zhuang, Z. W., Breuer, C. K., et al. (2010) · Science · doi:10.1126/science.1189345 · Cited by 1,177 · see citing works
Regeneration and orthotopic transplantation of a bioartificial lung
Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D., & Vacanti, J. P. (2010) · Nature Medicine · doi:10.1038/nm.2193 · Cited by 1,090 · see citing works
Extracellular matrix dynamics in development and regenerative medicine
Daley, W. P., Peters, S. B., & Larsen, M. (2008) · Journal of Cell Science · doi:10.1242/jcs.006064 · Cited by 972 · see citing works
Development of definitive endoderm from embryonic stem cells in culture
Kubo, A., Shinozaki, K., Shannon, J. M., Kouskoff, V., Kennedy, M., Woo, S. L. C., Fehling, H. J., & Keller, G. M. (2004) · Development · doi:10.1242/dev.01044 · Cited by 839 · see citing works
Vertebrate Endoderm Development and Organ Formation
Zorn, A. M., & Wells, J. M. (2009) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.042308.113344 · Cited by 812 · see citing works
Efficient generation of lung and airway epithelial cells from human pluripotent stem cells
Huang, S. X. L., Islam, M. N., O’Neill, J. D., Hu, Z., Yang, Y., Chen, Y., Mumau, M. D., Green, M. D., Vunjak‐Novakovic, G., Bhattacharya, J., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2754 · Cited by 565 · see citing works
p63+Krt5+ distal airway stem cells are essential for lung regeneration
Zuo, W., Zhang, T., Wu, D. Z., Guan, S. P., Liew, A.-A., Yamamoto, Y., Wang, X., Lim, S. J., Vincent, M., Lessard, M. D., et al. (2014) · Nature · doi:10.1038/nature13903 · Cited by 525 · see citing works
Defects in Tracheoesophageal and Lung Morphogenesis inNkx2.1(−/−) Mouse Embryos
Minoo, P., Su, G., Drum, H., Bringas, P., & Kimura, S. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9234 · Cited by 461 · see citing works
BMP-4 is required for hepatic specification of mouse embryonic stem cell–derived definitive endoderm
Gouon–Evans, V., Boussemart, L., Gadue, P. J., Nierhoff, D., Koehler, C. I., Kubo, A., Shafritz, D. A., & Keller, G. M. (2006) · Nature Biotechnology · doi:10.1038/nbt1258 · Cited by 430 · see citing works
Wong, A. P., Bear, C. E., Chin, S., Pasceri, P., Thompson, T., Huan, L., Ratjen, F. A., Ellis, J., & Rossant, J. (2012) · Nature Biotechnology · doi:10.1038/nbt.2328 · Cited by 425 · see citing works
Self-citationNiche‐mediated control of human embryonic stem cell self‐renewal and differentiation
Peerani, R., Rao, B. M., Bauwens, C. L., Yin, T., Wood, G. A., Nagy, A., Kumacheva, E., & Zandstra, P. W. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601896 · Cited by 390 · see citing works
Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells
Green, M. D., Chen, A. F., Nostro, M. C., D’Souza, S. L., Schaniel, C., Lemischka, I. R., Gouon–Evans, V., Keller, G. M., & Snoeck, H. W. (2011) · Nature Biotechnology · doi:10.1038/nbt.1788 · Cited by 388 · see citing works
Efficient Derivation of Purified Lung and Thyroid Progenitors from Embryonic Stem Cells
Longmire, T. A., Ikonomou, L., Hawkins, F., Christodoulou, C., Cao, Y., Jean, J. C., Kwok, L. W., Mou, H., Rajagopal, J., Shen, S. S., et al. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.01.019 · Cited by 384 · see citing works
Hong, K. U., Reynolds, S. D., Watkins, S. C., Fuchs, E. V., & Stripp, B. R. (2004) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00155.2003 · Cited by 375 · see citing works
Multiple roles for Sox2 in the developing and adult mouse trachea
Que, J., Luo, X., Schwartz, R. J., & Hogan, B. L. M. (2009) · Development · doi:10.1242/dev.034629 · Cited by 322 · see citing works
Mou, H., Zhao, R., Sherwood, R. I., Ahfeldt, T., Lapey, A., Wain, J. R., Sicilian, L., Izvolsky, K. I., Lau, F. H., Musunuru, K., et al. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.01.018 · Cited by 316 · see citing works
Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix
Ghaedi, M., Calle, E. A., Mendez, J. J., Gard, A. L., Balestrini, J. L., Booth, A., Bove, P. F., Gui, L., White, E. S., & Niklason, L. E. (2013) · Journal of Clinical Investigation · doi:10.1172/jci68793 · Cited by 230 · see citing works
Progenitor cells of the adult human airway involved in submucosal gland development
Engelhardt, J. F., Schlossberg, H., Yankaskas, J. R., & Dudus, L. (1995) · Development · doi:10.1242/dev.121.7.2031 · Cited by 203 · see citing works
Functional immobilization of signaling proteins enables control of stem cell fate
Alberti, K., Davey, R. E., Onishi, K., George, S. H. L., Salchert, K., Seib, F. P., Bornhäuser, M., Pompe, W., Nagy, A., Werner, C., et al. (2008) · Nature Methods · doi:10.1038/nmeth.1222 · Cited by 199 · see citing works
Gilpin, S. E., Ren, X., Okamoto, T., Guyette, J. P., Mou, H., Rajagopal, J., Mathisen, D. J., Vacanti, J. P., & Ott, H. C. (2014) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2014.05.080 · Cited by 130 · see citing works
Heparan sulfate–FGF10 interactions during lung morphogenesis
Izvolsky, K. I., Shoykhet, D., Yu, Y., Yu, Q., Nugent, M. A., & Cardoso, W. V. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00114-3 · Cited by 126 · see citing works
Normal lung development and function afterSox9 inactivation in the respiratory epithelium
Perl, A. T., Kist, R., Shan, Z., Scherer, G., & Whitsett, J. A. (2005) · genesis · doi:10.1002/gene.20093 · Cited by 97 · see citing works
Jensen, T., Roszell, B. R., Zang, F., Girard, E. D., Matson, A. P., Thrall, R. S., Jaworski, D. M., Hatton, C., Weiss, D. J., & Finck, C. M. (2012) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2011.0584 · Cited by 93 · see citing works
Key Mechanisms of Early Lung Development
Kimura, J., & Deutsch, G. H. (2007) · Pediatric and Developmental Pathology · doi:10.2350/07-06-0290.1 · Cited by 77 · see citing works
Kovacs, C. E., & Meyers, R. A. (2000) · Journal of Morphology · doi:10.1002/(sici)1097-4687(200005)244:2 · Cited by 55 · see citing works
Chondroitin sulfate proteoglycans are required for lung growth and morphogenesis in vitro
Shannon, J. M., McCormick‐Shannon, K., Burhans, M. S., Shangguan, X., Srivastava, K., & Hyatt, B. A. (2003) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00226.2003 · Cited by 40 · see citing works
Bilodeau, M., Shojaie, S., Ackerley, C. A., Post, M., & Rossant, J. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.07.010 · Cited by 32 · see citing works
Self-citationNew features in the development of the submucosal gland of the respiratory tract.
Smolich, J. J., Stratford, B. F., MALONEY, J. E., & Ritchie, B. C. (1978) · PubMed · Cited by 31 · see citing works
Toriyama, K., Muramatsu, H., Muramatsu, T., Torii, S., & Hoshino, T. (1997) · Differentiation · doi:10.1046/j.1432-0436.1997.6130161.x · Cited by 27 · see citing works
Heparan sulfate in lung morphogenesis: The elephant in the room
Thompson, S. M., Jesudason, E. C., Turnbull, J. E., & Fernig, D. G. (2010) · Birth Defects Research Part C Embryo Today Reviews · doi:10.1002/bdrc.20169 · Cited by 22 · see citing works
Anatomy of Clara cell secretion: surface changes observed by scanning electron microscopy.
Peão, M. N. D., Águas, A. P., Sá, C., & Grande, N. R. (1993) · Europe PMC (PubMed Central) · Cited by 20 · see citing works
Thompson, S. M., Connell, M. G., Fernig, D. G., ten Dam, G. B., Kuppevelt, T. H. V., Turnbull, J. E., Jesudason, E. C., & Losty, P. D. (2007) · Pediatric Surgery International · doi:10.1007/s00383-006-1864-8 · Cited by 20 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Extracellular matrix-based materials for regenerative medicine
Hussey, G. S., Dziki, J. L., & Badylak, S. F. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0023-x · Cited by 844 · see citing works
Bioprinting for vascular and vascularized tissue biofabrication
Datta, P., Ayan, B., & Ozbolat, I. T. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.01.035 · Cited by 431 · see citing works
Towards organoid culture without Matrigel
Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works
Konishi, S., Gotoh, S., Tateishi, K., Yamamoto, Y., Korogi, Y., Nagasaki, T., Matsumoto, H., Muro, S., Hirai, T., Ito, I., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.11.010 · Cited by 248 · see citing works
Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior
Cramer, M. C., & Badylak, S. F. (2020) · Annals of Biomedical Engineering · doi:10.1007/s10439-019-02408-9 · Cited by 196 · see citing works
Dye, B. R., Dedhia, P. H., Miller, A. J., Nagy, M. S., White, E. S., Shea, L. D., & Spence, J. R. (2016) · eLife · doi:10.7554/elife.19732 · Cited by 191 · see citing works
Controlling stem cell behavior with decellularized extracellular matrix scaffolds
Roth, G. A., & Christman, K. L. (2016) · Current Opinion in Solid State and Materials Science · doi:10.1016/j.cossms.2016.02.001 · Cited by 185 · see citing works
Decellularization for the retention of tissue niches
Moffat, D., Ye, K., & Jin, S. (2022) · Journal of Tissue Engineering · doi:10.1177/20417314221101151 · Cited by 152 · see citing works
Functional role of glycosaminoglycans in decellularized lung extracellular matrix
Uhl, F. E., Zhang, F., Pouliot, R. A., Uriarte, J. J., Enes, S. R., Han, X., Ouyang, Y., Xia, K., Westergren‐Thorsson, G., Malmström, A., et al. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.11.029 · Cited by 113 · see citing works
The Extracellular Matrix in Bronchopulmonary Dysplasia: Target and Source
Mižíková, I., & Morty, R. E. (2015) · Frontiers in Medicine · doi:10.3389/fmed.2015.00091 · Cited by 96 · see citing works
Tissue Engineering and Regenerative Medicine 2015: A Year in Review
Wobma, H., & Vunjak‐Novakovic, G. (2015) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2015.0535 · Cited by 92 · see citing works
The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform
Hussey, G. S., Keane, T. J., & Badylak, S. F. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.76 · Cited by 88 · see citing works
Zvarova, B., Uhl, F. E., Uriarte, J. J., Borg, Z. D., Coffey, A. L., Bonenfant, N. R., Weiss, D. J., & Wagner, D. E. (2016) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0439 · Cited by 87 · see citing works
Nano-Therapeutics for the Lung: State-of-the-Art and Future Perspectives
Iyer, R., Hsia, C. C. W., & Nguyen, K. T. (2015) · Current Pharmaceutical Design · doi:10.2174/1381612821666150923095742 · Cited by 76 · see citing works
Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application
Urbani, L., Maghsoudlou, P., Milan, À., Menikou, M., Hagen, C. K., Totonelli, G., Camilli, C., Eaton, S. D., Burns, A. J., Olivo, A., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0179341 · Cited by 66 · see citing works
How to build a lung: latest advances and emerging themes in lung bioengineering
Santis, M. M. D., Bölükbas, D. A., Lindstedt, S., & Wagner, D. E. (2018) · European Respiratory Journal · doi:10.1183/13993003.01355-2016 · Cited by 61 · see citing works
Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities
Li, K., He, Y., Jin, X., Jin, K., & Qian, J. (2025) · Journal of Translational Medicine · doi:10.1186/s12967-025-06349-x · Cited by 68 · see citing works
Novel detergent for whole organ tissue engineering
Kawasaki, T., Kirita, Y., Kami, D., Kitani, T., Ozaki, C., Itakura, Y., Toyoda, M., & Gojo, S. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35474 · Cited by 60 · see citing works
Revascularization of decellularized lung scaffolds: principles and progress
Stabler, C. T., Lecht, S., Mondrinos, M. J., Goulart, E., Lazarovici, P., & Lelkes, P. I. (2015) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00237.2015 · Cited by 59 · see citing works
Autophagy is required for lung development and morphogenesis
Yeganeh, B. Y., Lee, J., Ermini, L., Lok, I., Ackerley, C. A., & Post, M. (2019) · Journal of Clinical Investigation · doi:10.1172/jci127307 · Cited by 55 · see citing works
Self-citationUllah, I., Busch, J. F., Rabien, A., Ergün, B., Stamm, C., Knosalla, C., Hippenstiel, S., Reinke, P., & Kurtz, A. (2020) · Advanced Science · doi:10.1002/advs.201901198 · Cited by 50 · see citing works
Ullah, I., Abu‐Dawud, R., Busch, J. F., Rabien, A., Erguen, B., Fischer, I., Reinke, P., & Kurtz, A. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.119283 · Cited by 48 · see citing works
Alveolar-like Stem Cell–derived Myb − Macrophages Promote Recovery and Survival in Airway Disease
Litvack, M. L., Wigle, T. J., Lee, J., Wang, J., Ackerley, C. A., Grunebaum, E., & Post, M. (2016) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201509-1838oc · Cited by 46 · see citing works
Self-citationPlatz, J. J., Bonenfant, N. R., Uhl, F. E., Coffey, A. L., McKnight, T., Parsons, C. S., Sokocevic, D., Borg, Z. D., Lam, Y., Deng, B., et al. (2016) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2016.0109 · Cited by 45 · see citing works
Prieto, C., López‐Millán, B., Roca-Ho, H., Stam, R. W., Romero–Moya, D., Rodríguez‐Baena, F. J., Sanjuán-Pla, A., Ayllón, V., Ramírez, M. R., Bardini, M., et al. (2017) · Leukemia · doi:10.1038/leu.2017.294 · Cited by 43 · see citing works
Bioprinting of artificial blood vessels
Ng, H. Y., Lee, K.-X. A., Kuo, C. N., & Shen, Y. (2018) · International Journal of Bioprinting · doi:10.18063/ijb.v4i2.140 · Cited by 43 · see citing works
Lung bioengineering: advances and challenges in lung decellularization and recellularization
Uriarte, J. J., Uhl, F. E., Enes, S. R., Pouliot, R. A., & Weiss, D. J. (2018) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000584 · Cited by 42 · see citing works
Trevisan, C., Maghin, E., Dedja, A., Caccin, P., de Cesare, N., Franzin, C., Boso, D., Pesce, P., Caicci, F., Boldrin, F., et al. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.03.007 · Cited by 40 · see citing works
Júnior, C., Ulldemolins, A., Narciso, M., Almendros, I., Farré, R., Navajas, D., Alonso, J. L., Eroles, M., Rico, F., & Gavara, N. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24021708 · Cited by 39 · see citing works
Bilodeau, C., Shojaie, S., Goltsis, O., Wang, J., Luo, D., Ackerley, C. A., Rogers, I. M., Cox, B., & Post, M. (2021) · npj Regenerative Medicine · doi:10.1038/s41536-021-00124-4 · Cited by 37 · see citing works
Self-citationTan, C. L., Chan, Y., Candasamy, M., Chellian, J., Madheswaran, T., Sakthivel, L. P., Patel, V. K., Chakraborty, A., MacLoughlin, R. J., Kumar, D., et al. (2022) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2022.174821 · Cited by 37 · see citing works
Limitations of recellularized biological scaffolds for human transplantation
Bilodeau, C., Goltsis, O., Rogers, I. M., & Post, M. (2019) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.3004 · Cited by 35 · see citing works
Self-citationUltrasonic cavitation to prepare ECM hydrogels
Hussey, G. S., Nascari, D. G., Saldin, L. T., Kolich, B., Lee, Y. C., Crum, R. J., El-Mossier, S. O., D’Angelo, W., Dziki, J. L., & Badylak, S. F. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.03.036 · Cited by 35 · see citing works
Ultrasound in cellulose-based hydrogel for biomedical use: From extraction to preparation
Liu, Y., Li, S., Wang, Z., & Wang, L. (2022) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2022.112368 · Cited by 34 · see citing works
A novel decellularization method to produce brain scaffolds
Granato, A. E. C., da Cruz, E. F., Rodrigues‐Junior, D. M., Mosini, A. C., Ulrich, H., Rodrigues, B. V. M., Cheffer, A., & Porcionatto, M. A. (2020) · Tissue and Cell · doi:10.1016/j.tice.2020.101412 · Cited by 33 · see citing works
Sambi, M., Chow, T., Whiteley, J., Li, M., Chua, S. J., Raileanu, V., & Rogers, I. M. (2017) · Stem Cell Reviews and Reports · doi:10.1007/s12015-016-9712-2 · Cited by 33 · see citing works
Self-citationLaminin-driven Epac/Rap1 regulation of epithelial barriers on decellularized matrix
Young, B. M., Shankar, K., Tho, C. K., Pellegrino, A. R., & Heise, R. L. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.10.009 · Cited by 33 · see citing works
Chiu, Y., Shen, Y., Lee, A. K.-X., Lin, S.-H., Wu, Y.-C., & Chen, Y. (2019) · Polymers · doi:10.3390/polym11091394 · Cited by 30 · see citing works
Signaling involved in stem cell reprogramming and differentiation.
Tanabe, S. (2015) · PubMed Central · doi:10.4252/wjsc.v7.i7.992 · Cited by 29 · see citing works
Bongolan, T., Whiteley, J., Castillo-Prado, J., Fantin, A., Larsen, B. G., Wong, C. J., Mazilescu, L., Kawamura, M., Urbanellis, P., Jonebring, A., et al. (2022) · Biomaterials Science · doi:10.1039/d1bm01017d · Cited by 28 · see citing works
Self-citationUtilization of Natural Detergent Potassium Laurate for Decellularization in Lung Bioengineering
Obata, T., Tsuchiya, T., Akita, S., Kawahara, T., Matsumoto, K., Miyazaki, T., Masumoto, H., Kobayashi, E., Niklason, L. E., & Nagayasu, T. (2019) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2019.0016 · Cited by 27 · see citing works
Cardiac organoids: a new tool for disease modeling and drug screening applications
Yaqinuddin, A., Jabri, A., Mhannayeh, A., Taftafa, B., Alsharif, M., Abbad, T., Khan, J. A., Elsalti, A., Chinnappan, R., Alshehri, E. A., et al. (2025) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2025.1537730 · Cited by 27 · see citing works
Okuyama, H., Ohnishi, H., Nakamura, R., Yamashita, M., Kishimoto, Y., Tateya, I., Suehiro, A., Gotoh, S., Takezawa, T., Nakamura, T., et al. (2019) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2849 · Cited by 25 · see citing works
Leibel, S. L., & Post, M. (2016) · Frontiers in Pediatrics · doi:10.3389/fped.2016.00036 · Cited by 24 · see citing works
Self-citationInduced pluripotent stem cells for treating cystic fibrosis: State of the science
Pollard, B. S., & Pollard, H. B. (2018) · Pediatric Pulmonology · doi:10.1002/ppul.24118 · Cited by 24 · see citing works
Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells
LaRanger, R., Peters‐Hall, J. R., Coquelin, M. L., Alabi, B. R., Chen, C. T., Wright, W. E., & Shay, J. W. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2017.0114 · Cited by 23 · see citing works
Shakir, S., Hackett, T., & Mostaço-Guidolin, L. B. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1011800 · Cited by 22 · see citing works
Hoffman, E. T., Song, Y., Zhang, F., Asarian, L., Downs, I., Young, B., Han, X., Ouyang, Y., Xia, K., Linhardt, R. J., et al. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.06.043 · Cited by 22 · see citing works
State of the art on lung organoids in mammals
Archer, F., Erny, A., & Gomes, M. (2021) · Veterinary Research · doi:10.1186/s13567-021-00946-6 · Cited by 21 · see citing works
Chan, W. S., Mo, X.-T., Ip, P. P. C., & Tse, K. Y. (2023) · Cancer Medicine · doi:10.1002/cam4.6521 · Cited by 18 · see citing works
Ngan, S. Y., Quach, H. T., Laselva, O., Huang, E. N., Mangos, M., Xia, S., Bear, C. E., & Wong, A. P. (2022) · Current Protocols · doi:10.1002/cpz1.341 · Cited by 17 · see citing works
Self-citationThe long and winding road: stem cells for cystic fibrosis
Conese, M., Beccia, E., Castellani, S., Gioia, S. D., Colombo, C., Angiolillo, A., & Carbone, A. (2017) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2018.1413087 · Cited by 17 · see citing works
Lack of immunogenicity of xenogeneic DNA from porcine biomaterials
Record Ritchie, R. D., Salmon, S. L., Hiles, M. C., & Metzger, D. W. (2022) · Surgery Open Science · doi:10.1016/j.sopen.2022.07.005 · Cited by 16 · see citing works
Image-Based Method to Quantify Decellularization of Tissue Sections
Narciso, M., Otero, J., Navajas, D., Farré, R., Almendros, I., & Gavara, N. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168399 · Cited by 16 · see citing works
Explant Culture for Studying Lung Development
Yeganeh, B., Bilodeau, C., & Post, M. (2018) · Methods in molecular biology · doi:10.1007/978-1-0716-5126-1_6 · Cited by 15 · see citing works
Self-citationAdvanced single-cell technologies to guide the development of bioengineered lungs
Wanczyk, H., Jensen, T., Weiss, D. J., & Finck, C. M. (2021) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00089.2021 · Cited by 15 · see citing works
Singh, G., Senapati, S., Satpathi, S., Behera, P. K., Das, B., & Nayak, B. (2022) · The FASEB Journal · doi:10.1096/fj.202200807r · Cited by 15 · see citing works
Bölükbas, D. A., Santis, M. M. D., Alsafadi, H. N., Doryab, A., & Wagner, D. E. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9086-3_20 · Cited by 15 · see citing works
Options for modeling the respiratory system: inserts, scaffolds and microfluidic chips
Sedláková, V., Kloučková, M., Garlíková, Z., Vašíčková, K., Jaroš, J., Kandra, M., Kotasová, H., & Hampl, A. (2019) · Drug Discovery Today · doi:10.1016/j.drudis.2019.03.006 · Cited by 15 · see citing works
Hedström, U., Öberg, L., Vaarala, O., Dellgren, G., Silverborn, M., Bjermer, L. H., Westergren‐Thorsson, G., Hällgren, O., & Zhou, X. (2021) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2019-0395oc · Cited by 14 · see citing works
Organ-specific ECM arrays for investigating cell-ECM interactions during stem cell differentiation
Goh, S.-K., Halfter, W. M., Richardson, T., Bertera, S., Vaidya, V., Candiello, J. E., Bradford, M., & Banerjee, I. (2020) · Biofabrication · doi:10.1088/1758-5090/abc05f · Cited by 13 · see citing works
Calle, E. A., Leiby, K. L., Raredon, M. S. B., & Niklason, L. E. (2016) · Current Opinion in Anaesthesiology · doi:10.1097/aco.0000000000000425 · Cited by 13 · see citing works
Goh, S.-K., Bertera, S., Vaidya, V., Dumpe, S., Barner, S., Mathew, S., & Banerjee, I. (2018) · TECHNOLOGY · doi:10.1142/s2339547818500085 · Cited by 13 · see citing works
Ozcebe, S. G., Tristan, M., & Zorlutuna, P. (2025) · Stem Cells · doi:10.1093/stmcls/sxaf005 · Cited by 12 · see citing works
Conversion of human and mouse fibroblasts into lung-like epithelial cells
Wong, A. P., Shojaie, S., Liang, Q., Xia, S., Paola, M. D., Ahmadi, S., Bilodeau, C., Garner, J., Post, M., Duchesneau, P., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-45195-y · Cited by 12 · see citing works
Self-citationSzulc, D. A., Ahmadipour, M., Aoki, F. G., Waddell, T. K., Karoubi, G., & Cheng, H. M. (2019) · Magnetic Resonance in Medicine · doi:10.1002/mrm.28072 · Cited by 12 · see citing works
Noori, A., Dezfouli, M. R. M., Rajabi, S., Ganji, F., Ghezelayagh, Z., Agha, E. E., Baharvand, H., Chaleshtori, S. S., & Tahamtani, Y. (2023) · PubMed Central · doi:10.22074/cellj.2023.563370.1148 · Cited by 10 · see citing works
Goh, S.-K., Bertera, S., Richardson, T., & Banerjee, I. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acb7bf · Cited by 11 · see citing works
Decellularizing and Recellularizing Adult Mouse Kidneys
Chow, T., Whiteley, J., & Rogers, I. M. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9021-4_15 · Cited by 9 · see citing works
Self-citationGharenaz, N. M., Movahedin, M., & Mazaheri, Z. (2021) · International Journal of Reproductive BioMedicine (IJRM) · doi:10.18502/ijrm.v19i4.9058 · Cited by 9 · see citing works
Emerging Paradigms in Bioengineering the Lungs
Mohgan, R., Candasamy, M., Mayuren, J., Singh, S. K., Gupta, G., Dua, K., & Chellappan, D. K. (2022) · Bioengineering · doi:10.3390/bioengineering9050195 · Cited by 9 · see citing works
Influence of mesenchymal and biophysical components on distal lung organoid differentiation
Goltsis, O., Bilodeau, C., Wang, J., Luo, D., Asgari, M., Bozec, L., Pettersson, A. B. V., Leibel, S. L., & Post, M. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03890-2 · Cited by 8 · see citing works
Self-citationGeneration of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
Shojaie, S., Lee, J., Wang, J., Ackerley, C. A., & Post, M. (2016) · Journal of Visualized Experiments · doi:10.3791/54019 · Cited by 8 · see citing works
Self-citationCurrent Status of Bioprinting Using Polymer Hydrogels for the Production of Vascular Grafts
Matějková, J., Kaňoková, D., & Matějka, R. (2024) · Gels · doi:10.3390/gels11010004 · Cited by 7 · see citing works
Decellularized diseased tissues: current state‐of‐the‐art and future directions
Li, X., Shan, J., Chen, X., Cui, H., Wen, G., & Yu, Y. (2023) · MedComm · doi:10.1002/mco2.399 · Cited by 6 · see citing works
Tissue Engineering of the Trachea: What is the Hold-up?
Siddiqi, S. (2017) · MOJ Cell Science & Report · doi:10.15406/mojcsr.2017.04.00076 · Cited by 7 · see citing works
Granath, C., Noren, H., Björck, H. M., Simon, N. L., Olesen, K., Rodin, S., Grinnemo, K., & Österholm, C. (2020) · BioResearch open access · doi:10.1089/biores.2020.0018 · Cited by 7 · see citing works
Yang, X., Zhao, Y., Liu, W., Gao, Z., Wang, C., Wang, C., Li, S., & Zhang, X. (2024) · Biophysics Reports · doi:10.52601/bpr.2024.240011 · Cited by 5 · see citing works
Santis, M. M. D., Michielin, F., Shibuya, S., Coppi, P. D., & Wagner, D. E. (2021) · European Respiratory Society eBooks · doi:10.1183/2312508x.10011020 · Cited by 5 · see citing works
Adult Human Heart ECM Improves Human iPSC-CM Function via Mitochondrial and Metabolic MaturationPreprint
Ozcebe, S. G., Tristan, M., & Zorlutuna, P. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.31.565062 · Cited by 5 · see citing works
Sabetkish, S., & Kajbafzadeh, A. M. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-82735-9_4 · Cited by 3 · see citing works
The Extracellular Matrix in Development
Shojaie, S., Leibel, S. L., & Post, M. (2016) · Elsevier eBooks · doi:10.1016/b978-0-323-35214-7.00005-6 · Cited by 3 · see citing works
Self-citationEngineering Bioartificial Lungs for Transplantation
Wu, T., Economopoulos, K. P., & Ott, H. C. (2017) · Current Stem Cell Reports · doi:10.1007/s40778-017-0082-8 · Cited by 3 · see citing works
Regenerative Medicine of the Respiratory Tract
Gilpin, S. E., Moser, P. T., & Ott, H. C. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809880-6.00060-6 · Cited by 2 · see citing works
Spatiotemporal Extracellular Matrix Modeling forin SituCell Niche Studies
Olesen, K., Rodin, S., Mak, W. C., Felldin, U., Österholm, C., Tilevik, A., & Grinnemo, K.-H. (2021) · Stem Cells · doi:10.1002/stem.3448 · Cited by 2 · see citing works
Méthodes alternatives in vitro pour l’étude des interactions hôte-pathogène du poumon
Rémot, A., Descamps, D., Erny, A., Chottin, C., Drajac, C., Carreras, F., Ferret, C., & Archer, F. (2023) · INRAE Productions Animales · doi:10.20870/productions-animales.2023.36.2.7574 · Cited by 1 · see citing works
Development and Bioengineering of Lung Regeneration
Wilson, A. A., & Ikonomou, L. (2017) · Springer eBooks · doi:10.1007/978-981-10-3768-9_13 · Cited by 1 · see citing works
Bioprinting of vascularized tissues
Wu, Y. B., Fuh, J. Y. H., & Ozbolat, I. T. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-824291-9.00005-1 · Cited by 1 · see citing works
Chemical approaches to stem cell and signaling pathways for therapeutics
Paul, S., & Majhi, A. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-13212-4.00030-1 · Cited by 1 · see citing works
Mesenchymal Stromal Cell Therapy for Thoracic Surgeons: An Update
Petrella, F., Cassina, E. M., Libretti, L., Pirondini, E., Raveglia, F., & Tuoro, A. (2023) · Journal of Personalized Medicine · doi:10.3390/jpm13121632 · Cited by 1 · see citing works
Design and development of miRNA and stem cell based micro/nano systems as lung disease therapy
Alhasan, L. (2016) · RMIT Research Repository (RMIT University Library) · doi:10.25439/rmt.27582078
Regenerating the Respiratory Tract
Lim, M. L., Firsova, A. B., Feliu, N., Kuevda, E. V., Jungebluth, P., & Macchiarini, P. (2015) · Stem cell biology and regenerative medicine · doi:10.1007/978-3-319-21082-7_17
Tissue Engineering Architectural Cues for in vitro Models of Respiratory Epithelium
Poon, J. C. H. (2018) · TSpace (University of Toronto)
Stem cell-driven tissue engineering in lung engineering, regeneration, and diseases
El-Hashash, A. H. K. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821206-6.00008-4
Fantin, A. (2020) · TSpace (University of Toronto)
A Maverick Review of Common Stem/Progenitor Markers in Lung Development
Lin, Y., Wang, D., & Zeng, Y. (2022) · Stem Cell Reviews and Reports · doi:10.1007/s12015-022-10422-z
Modeling Lung Development and Diseases with Lung OrganoidsPreprint
Zhou, L., Liu, D., Wei, J., Zhu, X., & Jiang, L. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4581586
Ahmadipour, M., Castillo-Prado, J., Hakak‐Zargar, B., Mahmood, M. Q., & Rogers, I. M. (2024) · Biotechnology and Bioengineering · doi:10.1002/bit.28754
Self-citationAdult stem cells in regenerative medicine of respiratory system
Yaghoubi, A., Nazarnezhad, S., Asgharzadeh, F., Saeinasab, M., & Sefat, F. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-34152-6.00008-1
- Authors
- Shojaie, S., Ermini, L., Ackerley, C., Wang, J., Chin, S., Yeganeh, B., Bilodeau, M., Sambi, M., Rogers, I., Rossant, J., Bear, C. E., & Post, M.
- Affiliations
- University of Toronto (CA)
- Hospital for Sick Children (CA)
- Mount Sinai Hospital (CA)
- Corresponding author
- Post, Martin
- ORCID
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 4
- Issue
- 3
- Pages
- 419-430
- ISSN
- 2213-6711 (ISSN-L)
- PMC
- PMC4375883
- OpenAlex
- W2012377092
- Cited by
- 102
- References
- 37
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 12 · 2022: 10 · 2021: 11 · 2020: 7 · 2019: 15 · 2018: 9 · 2017: 10 · 2016: 11 · 2015: 8
- FWCI
- 12.75
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Use Siena air (University of Siena) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- decellularized lung scaffolds, airway epithelial differentiation, definitive endoderm, heparan sulfate proteoglycans, extracellular matrix, ciliated cells, Clara cells, basal cells, stem cell differentiation, lung development, serum-free medium
- MeSH terms
- Thyroid Nuclear Factor 1, Animals, Cell Differentiation, Cells, Cultured, Endoderm, Epithelial Cells, Extracellular Matrix, Lung, Mice, Transgenic, Nuclear Proteins, Protein Binding, Stem Cells, Transcription Factors, Cystic Fibrosis Transmembrane Conductance Regulator, Cell Lineage, Heparan Sulfate Proteoglycans, Respiratory Mucosa, Mice, SOXB1 Transcription Factors, Alveolar Epithelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
