Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior
Open accessAbstract
Biologic scaffold materials composed of allogeneic or xenogeneic extracellular matrix (ECM) are commonly used for the repair and remodeling of injured tissue. The clinical outcomes associated with implantation of ECM-based materials range from unacceptable to excellent. The variable clinical results are largely due to differences in the preparation of the material, including characteristics of the source tissue, the method and efficacy of decellularization, and post-decellularization processing steps. The mechanisms by which ECM scaffolds promote constructive tissue remodeling include mechanical support, degradation and release of bioactive molecules, recruitment and differentiation of endogenous stem/progenitor cells, and modulation of the immune response toward an anti-inflammatory phenotype. The methods of ECM preparation and the impact of these methods on the quality of the final product are described herein. Examples of favorable cellular responses of immune and stem cells associated with constructive tissue remodeling of ECM bioscaffolds are described.
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In the absence of infection, metabolic disorder, or pharmacological immunosuppression, the immune response to an implanted ECM bioscaffold is primarily determined by the material's biochemical composition, structural integrity, and associated molecular cues.
“In the absence of infection, metabolic disorder, or pharmacological immunosuppression, the immune response to an implanted ECM bioscaffold is primarily determined by the material's biochemical composition, structural integrity, and associated molecular cues [1,99].”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [1]
The Favorable Cellular Response and Immune Cells sections (page not provided) link scaffold source, processing, retained structure, and bioactive components to the host immune response. They do not establish that these material properties are the primary determinants specifically in the absence of infection, metabolic disorder, or pharmacological immunosuppression. (LLM, conf=0.84)
Experimental — accuracy not yet measuredThe immunomodulatory properties of ECM bioscaffolds depend on source tissue and decellularization methods.
“However, the immunomodulatory properties of ECM bioscaffolds are dependent on the source tissue and, critically, on the methods used for decellularization.”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [1]
ECM bioscaffolds have demonstrated therapeutic potential across clinical and preclinical applications for tissue repair and regeneration.
“Extracellular matrix (ECM) bioscaffolds have demonstrated therapeutic potential across a variety of clinical and preclinical applications for tissue repair and regeneration.”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [1]
ECM bioscaffolds and their components can modulate the immune response.
“In parallel, these scaffolds and their components have shown the capacity to modulate the immune response.”
From Immune Response to Extracellular Matrix Bioscaffolds: A Comprehensive Review (2025) · cites this paper as [1]
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- Authors
- Cramer, M. C., & Badylak, S. F.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Badylak, Stephen F.
- Venue
- Annals of Biomedical Engineering
- Published
- 2019-11-18
- Volume
- 48
- Issue
- 7
- Pages
- 2132-2153
- ISSN
- 0090-6964 (ISSN-L)
- 1573-9686
- PMC
- PMC7231673
- OpenAlex
- W2984209949
- Cited by
- 196
- References
- 305
- Citations by year
- 2026: 23 · 2025: 35 · 2024: 33 · 2023: 34 · 2022: 27 · 2021: 29 · 2020: 14 · 2019: 1
- FWCI
- 16.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Annals of Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (5T32HL076124-12)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- extracellular matrix scaffolds, decellularization, tissue engineering biomaterials, tissue remodeling, stem cell recruitment, progenitor cell differentiation, immunomodulation, anti-inflammatory phenotype, mechanical support
- MeSH terms
- Animals, Biocompatible Materials, Cell Differentiation, Extracellular Matrix, Humans, Stem Cells, Tissue Engineering, Tissue Scaffolds
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