Nucleobase Pairing in Expanded Watson-Crick-like Genetic Information Systems
Open accessAbstract
To guide the design of alternative genetic systems, we measured melting temperatures of DNA duplexes containing matched and mismatched nucleobase pairs from natural and unnatural structures. The pairs were analyzed in terms of structural features, including nucleobase size, number of hydrogen bonds formed, the presence of uncompensated hydrogen bonding functional groups, the nature of the bond joining the nucleobase to the sugar, and nucleobase charge. The results suggest that stability of nucleobase pairs correlates with the number of H-bonds, size complementarity, the presence of uncompensated functional groups, and the presence of charge on a nucleobase. Each of these properties appear to be more significant than the nature of the glycosidic bond and sequence context. The results provide guidelines for constructing stable Watson-Crick like nucleobase pairs with unnatural nucleobases. The experiments also demonstrate that expanded genetic systems can be constructed using size complementary nucleobase pairs that contain three hydrogen bonds.
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Another alternative genetic system could also have a genetic polymer with different bases than the four used on Earth or with a different chemical backbone.
“Another alternative genetic system could also have a genetic polymer (i.e., DNA-like molecule) with different bases than the four used on Earth or with a different chemical backbone (Geyer et al., 2003; Benner et al., 2004).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Geyer et al., 2003
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- Authors
- Geyer, C. R., Battersby, T. R., & Benner, S. A.
- Affiliations
- University of Florida (US)
- Corresponding author
- Geyer, C.Ronald
- Venue
- Structure
- Publisher
- Elsevier BV
- Published
- 2003-12
- Volume
- 11
- Issue
- 12
- Pages
- 1485-1498
- ISSN
- 0969-2126 (ISSN-L)
- OpenAlex
- W2054436276
- Cited by
- 159
- References
- 60
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 8 · 2023: 6 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 11 · 2017: 6 · 2016: 9 · 2015: 7 · 2014: 5 · 2013: 14 · 2012: 5
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Structure (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM-54048, R01GM054048)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
- Keywords
- nucleobase pairing, unnatural nucleobases, Watson-Crick base pairing, mismatched base pairs, hydrogen bonding, glycosidic linkage
- MeSH terms
- DNA, Hydrogen Bonding, Information Systems, Models, Chemical, Nucleic Acid Conformation, Protein Conformation, Purines, Pyrimidines, Temperature, Thermodynamics, Base Pairing
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