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Cited by 11Microtubule and mitosis dynamicsProtein Structure and DynamicsNuclear Structure and Function

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In vitro characterization of the full-length human dynein-1 cargo adaptor BicD2

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Fagiewicz, Robert, Crucifix, Corinne, Klos, Torben, Deville, Célia, Kieffer, Bruno, Nominé, Yves, Busselez, Johan, Rossolillo, Paola, Schmidt, Helgo·2022·Structure
DOI 10.1016/j.str.2022.08.009

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Abstract

Cargo adaptors are crucial in coupling motor proteins with their respective cargos and regulatory proteins. BicD2 is a prominent example within the cargo adaptor family. BicD2 is able to recruit the microtubule motor dynein to RNA, viral particles, and nuclei. The BicD2-mediated interaction between the nucleus and dynein is implicated in mitosis, interkinetic nuclear migration (INM) in radial glial progenitor cells, and neuron precursor migration during embryonic neocortex development. In vitro studies involving full-length cargo adaptors are difficult to perform due to the hydrophobic character, low-expression levels, and intrinsic flexibility of cargo adaptors. Here, we report the recombinant production of full-length human BicD2 and confirm its biochemical activity by interaction studies with RanBP2. We also describe pH-dependent conformational changes of BicD2 using cryoelectron microscopy (cryo-EM), template-free structure predictions, and biophysical tools. Our results will help define the biochemical parameters for the in vitro reconstitution of higher-order BicD2 protein complexes.

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Metadata

Authors
Fagiewicz, R., Crucifix, C., Klos, T., Deville, C., Kieffer, B., Nominé, Y., Busselez, J., Rossolillo, P., & Schmidt, H.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Schmidt, Helgo
ORCID
Venue
Structure
Publisher
Elsevier BV
Published
2022-11
Volume
30
Issue
11
Pages
1470-1478.e3
ISSN
0969-2126 (ISSN-L)
DOI
10.1016/j.str.2022.08.009
OpenAlex
W4296663409
Cited by
11
References
1
Citations by year
2026: 4 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 1
FWCI
0.94
Type
journal article
Language
en
Open access
Licence
Locations
  • Structure (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by-nc)
Funders
Agence Nationale de la Recherche (ANR-10-IDEX-0002, ANR-20-SFRI-0012, ANR-17-EURE-0023, ANR-18-EURE-0023, ANR-10-LABX-0030, ANR-10-INBS-0005)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
BICD2, cargo adaptors, cytoplasmic dynein, RanBP2, microtubule motor proteins, recombinant protein expression, pH-dependent conformational change, interkinetic nuclear migration, protein complex reconstitution, cryo-EM structure
MeSH terms
Dynactin Complex, Dyneins, Humans, Microtubule-Associated Proteins, Microtubules, Cryoelectron Microscopy

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