Bicaudal-D regulates COPI- independent Golgi-ER transport by recruiting the dynein-dynactin motor complex
Abstract
The small GTPase Rab6a is involved in the regulation of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER) in a coat complex coatomer protein I (COPI)-independent pathway. Here, we used a yeast two-hybrid approach to identify binding partners of Rab6a. In particular, we identified the dynein-dynactin-binding protein Bicaudal-D1 (BICD1), one of the two mammalian homologues of Drosophila Bicaudal-D. BICD1 and BICD2 colocalize with Rab6a on the trans-Golgi network (TGN) and on cytoplasmic vesicles, and associate with Golgi membranes in a Rab6-dependent manner. Overexpression of BICD1 enhances the recruitment of dynein-dynactin to Rab6a-containing vesicles. Conversely, overexpression of the carboxy-terminal domain of BICD, which can interact with Rab6a but not with cytoplasmic dynein, inhibits microtubule minus-end-directed movement of green fluorescent protein (GFP)-Rab6a vesicles and induces an accumulation of Rab6a and COPI-independent ER cargo in peripheral structures. These data suggest that coordinated action between Rab6a, BICD and the dynein-dynactin complex controls COPI-independent Golgi-ER transport.
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Rab6A participates in the motility of post-Golgi, exocytic transport vesicles in cooperation with KIF5B and with cytoplasmic dynein via bicaudal D1 and D2 proteins.
“Rab6A participates in the motility of post-Golgi, exocytic transport vesicles (Grigoriev et al., 2007) in cooperation with the kinesin motor, KIF5B, and with cytoplasmic dynein via its effector, bicaudal D1 and D2 proteins (Matanis et al., 2002).”
From The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization (2015) · cites this paper as Matanis et al., 2002
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- Authors
- Matanis, T., Akhmanova, A., Wulf, P., Del Nery, E., Weide, T., Stepanova, T., Galjart, N., Grosveld, F., Goud, B., De Zeeuw, C. I., Barnekow, A., & Hoogenraad, C. C.
- Affiliations
- University of Münster (DE)
- Erasmus University Rotterdam (NL)
- Centre National de la Recherche Scientifique (FR)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Akhmanova, Anna
- Hoogenraad, Casper C.
- Venue
- Nature Cell Biology
- Published
- 2002-11-25
- Volume
- 4
- Issue
- 12
- Pages
- 986-992
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2047730219
- Cited by
- 413
- References
- 24
- Citations by year
- 2026: 12 · 2025: 11 · 2024: 11 · 2023: 14 · 2022: 15 · 2021: 16 · 2020: 26 · 2019: 29 · 2018: 16 · 2017: 16 · 2016: 10 · 2015: 15 · 2014: 25 · 2013: 12 · 2012: 13
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Update notices
- erratum (2003-01-01)
- Open access
- closed
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- Rab6A, dynein-dynactin complex, trans-Golgi network, membrane trafficking, vesicle trafficking
- MeSH terms
- Dynactin Complex, Animals, Biological Transport, Dyneins, Endoplasmic Reticulum, Golgi Apparatus, HeLa Cells, Humans, Microtubule-Associated Proteins, Molecular Sequence Data, COS Cells, Molecular Motor Proteins, rab GTP-Binding Proteins, Coat Protein Complex I, Coatomer Protein, Drosophila Proteins
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