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Activation loop phosphorylation tunes conformational dynamics underlying Pyk2 tyrosine kinase activation
Open accessPublished version of a preprint
Abstract
Pyk2 is a multidomain non-receptor tyrosine kinase that undergoes a multistage activation mechanism. Activation is instigated by conformational rearrangements relieving autoinhibitory FERM domain interactions. The kinase autophosphorylates a central linker residue to recruit Src kinase. Pyk2 and Src mutually phosphorylate activation loops to confer full activation. While the mechanisms of autoinhibition are established, the conformational dynamics associated with autophosphorylation and Src recruitment remain unclear. We employ hydrogen/deuterium exchange mass spectrometry and kinase activity profiling to map the conformational dynamics associated with substrate binding and Src-mediated activation loop phosphorylation. Nucleotide engagement stabilizes the autoinhibitory interface, while phosphorylation deprotects both FERM and kinase regulatory surfaces. Phosphorylation organizes active site motifs linking catalytic loop with activation segment. Dynamics of the activation segment anchor propagate to EF/G helices to prevent reversion of the autoinhibitory FERM interaction. We employ targeted mutagenesis to dissect how phosphorylation-induced conformational rearrangements elevate kinase activity above the basal autophosphorylation rate.
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Self-citation
Metadata
- Authors
- Palhano Zanela, T. M., Woudenberg, A., Romero Bello, K. G., & Underbakke, E. S.
- Affiliations
- Iowa State University (US)
- Corresponding author
- Underbakke, Eric S.
- Venue
- Structure
- Publisher
- Elsevier BV
- Published
- 2023-04
- Volume
- 31
- Issue
- 4
- Pages
- 447-454.e5
- ISSN
- 0969-2126 (ISSN-L)
- OpenAlex
- W4323038605
- Cited by
- 12
- References
- 44
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 4
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 371 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Structure (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Iowa State University Digital Repository (Iowa State University) (submittedVersion)
- Funders
- Division of Molecular and Cellular Biosciences (1715411)
- National Science Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Keywords
- Pyk2, non-receptor tyrosine kinase, kinase activation, activation loop phosphorylation, conformational dynamics, Src kinase, autophosphorylation, nucleotide binding, hydrogen-deuterium exchange, substrate binding, kinase profiling
- MeSH terms
- Molecular Conformation, Phosphorylation, Tyrosine, src-Family Kinases, Focal Adhesion Kinase 2
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