Cited by 27Genomics and Chromatin DynamicsDiffusion and Search DynamicsForce Microscopy Techniques and Applications

Structural dynamics of nucleosomes at single-molecule resolution

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Choy, John S., Lee, Tae-Hee·2012·Trends in Biochemical Sciences

Abstract

The detailed mechanisms of how DNA that is assembled around a histone core can be accessed by DNA-binding proteins for transcription, replication, or repair, remain elusive nearly 40 years after Kornberg's nucleosome model was proposed. Uncovering the structural dynamics of nucleosomes is a crucial step in elucidating the mechanisms regulating genome accessibility. This requires the deconvolution of multiple structural states within an ensemble. Recent advances in single-molecule methods enable unprecedented efficiency in examining subpopulation dynamics. In this review, we summarize studies of nucleosome structure and dynamics from single-molecule approaches and how they advance our understanding of the mechanisms that govern DNA transactions.

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Authors
Choy, J. S., & Lee, T.-H.
Affiliations
  • Stanford University (US)
  • Pennsylvania State University (US)
Corresponding author
  • Choy, John S.
  • Lee, Tae-Hee
Venue
Trends in Biochemical Sciences
Publisher
Elsevier BV
Published
2012-10
Volume
37
Issue
10
Pages
425-435
ISSN
0968-0004 (ISSN-L)
OpenAlex
W2023860198
Cited by
27
References
84
Citations by year
2026: 1 · 2025: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 3 · 2017: 2 · 2016: 7 · 2015: 6 · 2013: 1
FWCI
0.20
Type
journal article
Language
en
Open access
Locations
  • Trends in Biochemical Sciences (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (R01GM097286, GM097286)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords
nucleosome structure, nucleosome dynamics, histone core, DNA-binding proteins, DNA accessibility, genome accessibility, transcription, DNA replication, DNA repair
MeSH terms
DNA, DNA-Binding Proteins, Humans, Models, Molecular, Nucleic Acid Conformation, Nucleosomes

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