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Location, Location, Location: Compartmentalization of NAD+ Synthesis and Functions in Mammalian Cells
Open accessAbstract
The numerous biological roles of NAD+ are organized and coordinated via its compartmentalization within cells. The spatial and temporal partitioning of this intermediary metabolite is intrinsic to understanding the impact of NAD+ on cellular signaling and metabolism. We review evidence supporting the compartmentalization of steady-state NAD+ levels in cells, as well as how the modulation of NAD+ synthesis dynamically regulates signaling by controlling subcellular NAD+ concentrations. We further discuss potential benefits to the cell of compartmentalizing NAD+, and methods for measuring subcellular NAD+ levels.
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“NAD+ is highly compartmentalized in the cytoplasm, mitochondria and nucleus, which represent its main subcellular pools (Supplementary Box 1).”
From NAD+ metabolism and its roles in cellular processes during ageing (2021) · cites this paper as [2]
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“These pools are regulated independently of each other, and consistent with this, the enzymes involved in the biosynthesis or degradation of NAD+ are highly compartmentalized as well2–4.”
From NAD+ metabolism and its roles in cellular processes during ageing (2021) · cites this paper as [2]
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Metadata
- Authors
- Cambronne, X. A., & Kraus, W. L.
- Affiliations
- The University of Texas at Austin (US)
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Cambronne, Xiaolu A.
- Kraus, W. Lee
- Venue
- Trends in Biochemical Sciences
- Publisher
- Elsevier BV
- Published
- 2020-10
- Volume
- 45
- Issue
- 10
- Pages
- 858-873
- ISSN
- 0968-0004 (ISSN-L)
- PMC
- PMC7502477
- OpenAlex
- W3037844642
- Cited by
- 137
- References
- 130
- Citations by year
- 2026: 25 · 2025: 23 · 2024: 26 · 2023: 25 · 2022: 21 · 2021: 15 · 2020: 2
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Trends in Biochemical Sciences (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- NAD+, compartmentalization, NAD+ synthesis, cellular metabolism, signal transduction, subcellular compartmentalization
- MeSH terms
- Animals, Cell Compartmentation, NAD, Subcellular Fractions, Signal Transduction
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