The DOI matches its Crossref record; 81 of the 83 references it cites were found in a registry or an index.
Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding
Abstract
Conventional plant breeding exploits existing genetic variability and introduces new variability by mutagenesis. This has proven highly successful in securing food supplies for an ever-growing human population. The use of genetically modified plants is a complementary approach but all plant breeding techniques have limitations. Here, we discuss how the recent evolution of targeted mutagenesis and DNA insertion techniques based on tailor-made site-directed nucleases (SDNs) provides opportunities to overcome such limitations. Plant breeding companies are exploiting SDNs to develop a new generation of crops with new and improved traits. Nevertheless, some technical limitations as well as significant uncertainties on the regulatory status of SDNs may challenge their use for commercial plant breeding.
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In the citing paper
“SDN3 mutants use a repair template with homologous regions of at least 500 base pairs flanking the DSB, enabling the insertion of new sequences, similar to transgenic or cisgenic approaches.”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Podevin et al., 2013
In the citing paper
“Nonetheless, SDN3 applications in plants face challenges due to the low efficiency of homologous recombination (HDR), making them complex and less efficient (Verma et al., 2020; Podevin et al., 2013; Van de Wiel et al., 2017).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Podevin et al., 2013
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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RNA-Guided Human Genome Engineering via Cas9
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Ciccia, A., & Elledge, S. J. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.09.019 · Cited by 4,368
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
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A Simple Cipher Governs DNA Recognition by TAL Effectors
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Double-Strand Break End Resection and Repair Pathway Choice
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High-efficiency TALEN-based gene editing produces disease-resistant rice
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Flagged first, then by citations
2 of the 83 references couldn’t be matched to a known paper.
Research works printed but not linked: 3
- Lusser, M. and Rodríguez-Cerezo, E. (2012) Comparative regulatory approaches for new plant breeding techniques. Workshop proceedings. European Commission. JRC Technical Report EUR 25237 EN
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Cited by
Sorted by citations
Kim, S., Kim, D., Cho, S. W., Kim, J., & Kim, J.-S. (2014) · Genome Research · doi:10.1101/gr.171322.113 · Cited by 1,856
The CRISPR/Cas9 system for plant genome editing and beyond
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Editing plant genomes with CRISPR/Cas9
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Global Regulation of Genetically Modified Crops Amid the Gene Edited Crop Boom – A Review
Turnbull, C., Lillemo, M., & Hvoslef‐Eide, A. K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.630396 · Cited by 479
The Regulatory Status of Genome‐edited Crops
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Metadata
- Authors
- Podevin, N., Davies, H. V., Hartung, F., Nogué, F., & Casacuberta, J. M.
- Affiliations
- European Food Safety Authority (IT)
- James Hutton Institute (GB)
- Julius Kühn-Institut (DE)
- AgroParisTech (FR)
- Universitat Autònoma de Barcelona (ES)
- Corresponding author
- Casacuberta, Josep M.
- Venue
- Trends in biotechnology
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 31
- Issue
- 6
- Pages
- 375-383
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W1992221498
- Cited by
- 214
- References
- 83
- Citations by year
- 2026: 11 · 2025: 20 · 2024: 28 · 2023: 11 · 2022: 34 · 2021: 16 · 2020: 14 · 2019: 14 · 2018: 10 · 2017: 8 · 2016: 17 · 2015: 12 · 2014: 9 · 2013: 9
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- OpenAgrar (submittedVersion)
- Funders
- European Food Safety Authority
- Agence Nationale de la Recherche (ANR-11-BTBR-0001)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- targeted mutagenesis, DNA insertion, plant breeding, transgenic plants, regulatory status, crop improvement
- MeSH terms
- Breeding, Deoxyribonucleases, Humans, Plants, Gene Targeting
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