Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

Boch, Jens; Scholze, Heidi; Schornack, Sebastian; Landgraf, Angelika; Hahn, Simone; Kay, Sabine; Lahaye, Thomas; Nickstadt, Anja; Bonas, Ulla

doi:10.1126/science.1178811

Cited by 2,470

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Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

Boch, Jens, Scholze, Heidi, Schornack, Sebastian, Landgraf, Angelika, Hahn, Simone, Kay, Sabine, Lahaye, Thomas, Nickstadt, Anja, Bonas, Ulla·2009·Science

DOI 10.1126/science.1178811

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Abstract

TAL Order: Xanthomonas bacteria attack their plant hosts by delivering their own transcription-activator–like (TAL) proteins into the plant cell nucleus and alter the plant's gene regulation (see the Perspective by Voytas and Joung ). Moscou and Bogdanove (p. 1501 , published online 29 October: see the cover) and Boch et al. (p. 1509 , published online 29 October) have now discovered how the similar but not identical repeats in the TAL proteins encode the specificity needed for the proteins to find their targets. Each repeat is specific for one DNA base pair, a specificity encoded by hypervariable amino acid positions. Combining several repeats with different amino acids in the hypervariable positions allowed the production of new effectors that targeted new DNA sites.

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TALENs were engineered through the fusion of the FokI domain with transcription activator-like (TAL) effectors, proteins found in plant pathogens whose DNA-binding domains (DBD) contain tandem repeats of 33-35 amino acids.
“On the other hand, TALENs were engineered through the fusion of the FokI domain with transcription activator-like (TAL) effectors, proteins found in plant pathogens whose DNA-binding domains (DBD) contain tandem repeats of 33–35 amino acids [89,90].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [90]

The plant immune system
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Authors: Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U.
Affiliations: Department of Genetics, Institute of Biology, Martin-Luther-University Halle-Wittenberg, Weinbergweg 10, D-06099 Halle (Saale) Germany.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2009-12-11
Volume: 326
Issue: 5959
Pages: 1509-1512
ISSN: 0036-8075
1095-9203
DOI: 10.1126/science.1178811
Cited by: 2,470
References: 1
Language: en

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Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

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