A 3D toolbox to enhance physiological relevance of human tissue models
Open accessAbstract
We discuss the current challenges and future prospects of flow-based organoid models and 3D self-assembling scaffolds. The existing paradigm of 3D culture suffers from a lack of control over organoid size and shape; can be an obstacle for cell harvesting and extended cellular and molecular analysis; and does not provide access to the function of exocrine glands. Moreover, existing organ-on-chip models are mostly composed of 2D extracellular matrix (ECM)-coated elastomeric membranes that do not mimic real organ architectures. A new comprehensive 3D toolbox for cell biology has emerged to address some of these issues. Advances in microfabrication and cell-culturing approaches enable the engineering of sophisticated models that mimic organ 3D architectures and physiological conditions, while supporting flow-based drug screening and secretomics-based diagnosis.
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These studies were performed on incomplete 3D models, cell aggregates such as MCTS or dissociated organoids devoid of any exogenous ECM.
“However, these studies were performed on incomplete 3D models, cell aggregates such as MCTS or dissociated organoids devoid of any exogenous ECM (40).”
From Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens (2018) · cites this paper as [40]
The Microfabrication section says hanging-drop microtissues form by artificial cell aggregation and usually lack matrices; the 3D Toolbox to Create Artificial Scaffolded Organs section also describes gel-free spheroid models. The paper does not establish that the particular studies referred to used those models, or that they used dissociated organoids devoid of exogenous ECM. Page numbers were not available in our copy. (LLM, conf=0.82)
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Self-citation
- Authors
- Picollet-D’hahan, N., Dolega, M. E., Liguori, L., Marquette, C., Le Gac, S., Gidrol, X., & Martin, D. K.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Lyon 1 Université (FR)
- Corresponding author
- Gidrol, Xavier
- Martin, Donald K.
- ORCID
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 34
- Issue
- 9
- Pages
- 757-769
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2506149650
- Cited by
- 65
- References
- 11
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 5 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 9 · 2017: 15 · 2016: 2
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- University of Twente Research Information (publishedVersion, other-oa)
- HAL (submittedVersion)
- University of Twente Research Information (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-11-NANB-0002, ANR-11-BSV5-0009)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- 3D tissue models, organoids, organ-on-a-chip, microfabrication, extracellular matrix, organ architecture, exocrine glands, secretomics, drug screening, cell harvesting
- MeSH terms
- Cells, Cultured, Extracellular Matrix, Humans, Models, Biological, Organoids, Cell Culture Techniques, Tissue Engineering, Microfluidic Analytical Techniques, Tissue Array Analysis, Tissue Scaffolds
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