Progress, obstacles, and limitations in the use of stem cells in organ-on-a-chip models

Multilineage potential of adult human mesenchymal stem cells

Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., & Marshak, D. R. (1999) · Science · doi:10.1126/science.284.5411.143 · Cited by 21,052 · see citing works

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors

Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221 · see citing works

Embryonic Stem Cell Lines Derived from Human Blastocysts

Thomson, J. A., Itskovitz‐Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,947 · see citing works

Matrix Metalloproteinase Inhibitors and Cancer—Trials and Tribulations

Coussens, L. M., Fingleton, B., & Matrisian, L. M. (2002) · Science · doi:10.1126/science.1067100 · Cited by 2,761 · see citing works

Circulating microRNAs in Sera Correlate with Soluble Biomarkers of Immune Activation but Do Not Predict Mortality in ART Treated Individuals with HIV-1 Infection: A Case Control Study

Murray, D. D., Suzuki, K., Law, M. G., Trebicka, J., Neuhaus, J., Wentworth, D. N., Johnson, M. A., Vjecha, M. J., Kelleher, A. D., Emery, S., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0139981 · Cited by 2,740 · see citing works

Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412

Suntharalingam, G., Perry, M. R., Ward, S., Brett, S. J., Castello-Cortes, A., Brunner, M. D., & Panoskaltsis, N. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa063842 · Cited by 2,164 · see citing works

From 3D cell culture to organs-on-chips

Huh, D., Hamilton, G. A., & Ingber, D. E. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.09.005 · Cited by 1,834 · see citing works

Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

Lian, X., Zhang, J., Azarin, S. M., Zhu, K., Hazeltine, L. B., Bao, X., Hsiao, C., Kamp, T. J., & Palecek, S. P. (2013) · Nature Protocols · doi:10.1038/nprot.2012.150 · Cited by 1,748 · see citing works

Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling

Lian, X., Hsiao, C., Wilson, G., Zhu, K., Hazeltine, L. B., Azarin, S. M., Raval, K. K., Zhang, J., Kamp, T. J., & Palecek, S. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1200250109 · Cited by 1,717 · see citing works

Chemically defined generation of human cardiomyocytes

Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2999 · Cited by 1,606 · see citing works

Induced pluripotent stem cell technology: a decade of progress.

Shi, Y., Inoue, H., Wu, J. C., & Yamanaka, S. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.245 · Cited by 1,525 · see citing works

Functional Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

Zhang, J.-H., Wilson, G. F., Soerens, A., Koonce, C. H., Yu, J.-Y., Palecek, S., Thomson, J., & Kamp, T. (2009) · Circulation Research · doi:10.1161/circresaha.108.192237 · Cited by 1,463 · see citing works

Modelling schizophrenia using human induced pluripotent stem cells

Brennand, K. J., Simone, A., Jou, J., Gelboin-Burkhart, C., Tran, N., Sangar, S., Li, Y., Mu, Y., Chen, G., Yu, D., et al. (2011) · Nature · doi:10.1038/nature09915 · Cited by 1,375 · see citing works

Three-dimensional cell culture: the missing link in drug discovery

Breslin, S., & O’Driscoll, L. (2013) · Drug Discovery Today · doi:10.1016/j.drudis.2012.10.003 · Cited by 1,332 · see citing works

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

Ronaldson-Bouchard, K., Ma, S. P., Yeager, K., Chen, T., Song, L., Sirabella, D., Morikawa, K., Teles, D., Yazawa, M., & Vunjak-Novakovic, G. (2018) · Nature · doi:10.1038/s41586-018-0016-3 · Cited by 1,272 · see citing works

Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines

Kattman, S. J., Witty, A. D., Gagliardi, M., Dubois, N. C., Niapour, M., Hotta, A., Ellis, J., & Keller, G. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2010.12.008 · Cited by 1,159 · see citing works

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Initiative, T. I. S. C. (2007) · Nature Biotechnology · doi:10.1038/nbt1318 · Cited by 1,078 · see citing works

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

Zhang, Y. S., Arneri, A., Bersini, S., Shin, S.-R., Zhu, K., Goli-Malekabadi, Z., Aleman, J., Colosi, C., Busignani, F., Dell’Erba, V., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.09.003 · Cited by 957 · see citing works

Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment

Jang, K., Mehr, A. P., Hamilton, G. A., McPartlin, L. A., Chung, S., Suh, K. Y., & Ingber, D. E. (2013) · Integrative Biology · doi:10.1039/c3ib40049b · Cited by 850 · see citing works

Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro

Benam, K. H., Villenave, R., Lucchesi, C., Varone, A., Hubeau, C., Lee, H., Alves, S. E., Salmon, M., Ferrante, T. C., Weaver, J. C., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3697 · Cited by 821 · see citing works

Human induced pluripotent stem cell–derived cardiomyocytes recapitulate the predilection of breast cancer patients to doxorubicin-induced cardiotoxicity

Burridge, P. W., Li, Y. F., Matsa, E., Wu, H., Ong, S., Sharma, A., Holmström, A., Chang, A. C. Y., Coronado, M. J., Ebert, A., et al. (2016) · Nature Medicine · doi:10.1038/nm.4087 · Cited by 746 · see citing works

Modeling Alzheimer’s Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness

Kondo, T., Asai, M., Tsukita, K., Kutoku, Y., Ohsawa, Y., Sunada, Y., Imamura, K., Egawa, N., Yahata, N., Okita, K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.01.009 · Cited by 741 · see citing works

Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells to Cardiomyocytes

Mummery, C. L., Zhang, J., Ng, E., Elliott, D. A., Elefanty, A. G., & Kamp, T. J. (2012) · Circulation Research · doi:10.1161/circresaha.110.227512 · Cited by 715 · see citing works

Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy

Sun, N., Yazawa, M., Liu, J., Han, L., Sanchez-Freire, V., Abilez, O. J., Navarrete, E. G., Hu, S., Wang, L., Lee, A., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003552 · Cited by 696 · see citing works

Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells

Patsch, C., Meylan, L. C., Thoma, E., Urich, E., Heckel, T., O’Sullivan, J., Grainger, S. J., Kapp, F., Sun, L., Christensen, K., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3205 · Cited by 628 · see citing works

Comparative proteomic phenotyping of cell lines and primary cells to assess preservation of cell type-specific functions

Pan, C., Kumar, C., Bohl, S., Klingmueller, U., & Mann, M. (2009) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m800258-mcp200 · Cited by 561 · cited 1× · see citing works

Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells

Egawa, N., Kitaoka, S., Tsukita, K., Naitoh, M., Takahashi, K., Yamamoto, T., Adachi, F., Kondo, T., Okita, K., Asaka, I., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004052 · Cited by 552 · see citing works

Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip

Musah, S., Mammoto, A., Ferrante, T. C., Jeanty, S. S. F., Hirano-Kobayashi, M., Mammoto, T., Roberts, K., Chung, S., Novak, R., Ingram, M., et al. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0069 · Cited by 550 · see citing works

Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

Sun, N., Panetta, N. J., Gupta, D. M., Wilson, K. D., Lee, A., Jia, F.-J., Hu, S.-J., Cherry, A., Robbins, R., Longaker, M., et al. (2009) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0908450106 · Cited by 539 · see citing works

Drug Screening Using a Library of Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Reveals Disease-Specific Patterns of Cardiotoxicity

Liang, P., Lan, F., Lee, A. S., Gong, T., Sánchez-Freire, V., Wang, Y., Diecke, S., Sallam, K., Knowles, J. W., Wang, P. J., et al. (2013) · Circulation · doi:10.1161/circulationaha.113.001883 · Cited by 535 · see citing works

Human iPSC-based Cardiac Microphysiological System For Drug Screening Applications

Mathur, A., Loskill, P., Shao, K., Huebsch, N., Hong, S., Marcus, S. G., Marks, N. C., Mandegar, M. A., Conklin, B. R., Lee, L. P., et al. (2015) · Scientific Reports · doi:10.1038/srep08883 · Cited by 527 · see citing works

Screening out irrelevant cell-based models of disease

Horvath, P., Aulner, N., Bickle, M., Davies, A. M., Nery, E. D., Ebner, D., Montoya, M. C., Östling, P., Pietiäinen, V., Price, L. S., et al. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.175 · Cited by 525 · see citing works

Microfluidic heart on a chip for higher throughput pharmacological studies

Agarwal, A., Goss, J. A., Cho, A., McCain, M. L., & Parker, K. K. (2013) · Lab on a Chip · doi:10.1039/c3lc50350j · Cited by 495 · see citing works

Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes: Insights Into Molecular, Cellular, and Functional Phenotypes

Karakikes, I., Ameen, M., Termglinchan, V., & Wu, J. C. (2015) · Circulation Research · doi:10.1161/circresaha.117.305365 · Cited by 478 · see citing works

Animal models of human cardiovascular disease, heart failure and hypertrophy

Hasenfuss, G. P. (1998) · Cardiovascular Research · doi:10.1016/s0008-6363(98)00110-2 · Cited by 437 · see citing works

A critical evaluation of in vitro cell culture models for high-throughput drug screening and toxicity

Astashkina, A. I., Mann, B. K., & Grainger, D. W. (2012) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2012.01.001 · Cited by 432 · see citing works

A micro cell culture analog (µCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs

Sung, J. H., & Shuler, M. L. (2009) · Lab on a Chip · doi:10.1039/b901377f · Cited by 431 · see citing works

Tri-iodo-l-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells

Yang, X., Rodriguez, M. L., Pabon, L., Fischer, K. A., Reinecke, H., Regnier, M., Sniadecki, N. J., Ruohola‐Baker, H., & Murry, C. E. (2014) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2014.04.005 · Cited by 430 · see citing works

A novel 3D mammalian cell perfusion-culture system in microfluidic channels

Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b614872g · Cited by 414 · see citing works

Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations

Lee, J. H., Protze, S. I., Laksman, Z., Backx, P. H., & Keller, G. M. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.07.003 · Cited by 404 · see citing works

High-throughput screening of tyrosine kinase inhibitor cardiotoxicity with human induced pluripotent stem cells

Sharma, A., Burridge, P. W., McKeithan, W. L., Serrano, R., Shukla, P., Sayed, N., Churko, J. M., Kitani, T., Wu, H., Holmström, A., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aaf2584 · Cited by 404 · see citing works

Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals

Zhang, Q., Jiang, J., Han, P., Yuan, Q., Zhang, J., Zhang, X., Xu, Y., Cao, H., Meng, Q., Chen, L., et al. (2010) · Cell Research · doi:10.1038/cr.2010.163 · Cited by 368 · see citing works

Real-time monitoring of metabolic function in liver-on-chip microdevices tracks the dynamics of mitochondrial dysfunction

Bavli, D., Prill, S., Tsur, E. E., Levy, G., Cohen, M., Vinken, M., Vanfleteren, J., Jaeger, M. S., & Nahmias, Y. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1522556113 · Cited by 366 · see citing works

Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker

Protze, S. I., Liu, J., Nussinovitch, U., Ohana, L., Backx, P. H., Gepstein, L., & Keller, G. M. (2017) · Nature Biotechnology · doi:10.1038/nbt.3745 · Cited by 366 · see citing works

Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

Zhang, C., Zhao, Z., Abdul Rahim, N. A., van Noort, D., & Yu, H. (2009) · Lab on a Chip · doi:10.1039/b915147h · Cited by 340 · see citing works

The Role of Body-on-a-Chip Devices in Drug and Toxicity Studies

Esch, M. B., King, T. L., & Shuler, M. L. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124629 · Cited by 333 · see citing works

Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

Lian, X. L., Bao, X., Al-Ahmad, A. J., Liu, J., Wu, Y., Dong, W., Dunn, K. K., Shusta, E. V., & Palecek, S. P. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.09.005 · Cited by 321 · see citing works

Monoclonal antibody TGN1412 trial failure explained by species differences in CD28 expression on CD4 ⁺ effector memory T‐cells

Eastwood, D., Findlay, L., Poole, S., Bird, C., Wadhwa, M., Moore, M., Burns, C., Thorpe, R., & Stebbings, R. (2010) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2010.00922.x · Cited by 300 · see citing works

Pluripotent stem cell-derived hepatocyte-like cells

Schwartz, R. E., Fleming, H. E., Khetani, S. R., & Bhatia, S. N. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.01.003 · Cited by 281 · see citing works

Placenta-on-a-chip: a novel platform to study the biology of the human placenta

Lee, J. S., Romero, R. J., Han, Y. M., Kim, H. C., Kim, C. J., Hong, J., & Huh, D. (2015) · The Journal of Maternal-Fetal & Neonatal Medicine · doi:10.3109/14767058.2015.1038518 · Cited by 272 · see citing works

Let-7 family of microRNA is required for maturation and adult-like metabolism in stem cell-derived cardiomyocytes

Kuppusamy, K. T., Jones, D. C., Sperber, H., Madan, A., Fischer, K. A., Rodriguez, M. L., Pabon, L., Zhu, W., Tulloch, N. L., Yang, X., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1424042112 · Cited by 265 · see citing works

Translation of Human iPSCs: From Clinical Trial in a Dish to Precision Medicine

Sayed, N., Liu, C., & Wu, J. C. (2016) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2016.01.083 · Cited by 263 · see citing works

Mesenchymal Stem Cell Preparations—Comparing Apples and Oranges

Wagner, W., & Ho, A. D. (2007) · Stem Cell Reviews and Reports · doi:10.1007/s12015-007-9001-1 · Cited by 260 · see citing works

Design and demonstration of a pumpless 14 compartment microphysiological system

Miller, P. G., & Shuler, M. L. (2016) · Biotechnology and Bioengineering · doi:10.1002/bit.25989 · Cited by 235 · see citing works

Animal models of chronic liver diseases

Liu, Y., Meyer, C., Xu, C., Weng, H., Hellerbrand, C., ten Dijke, P., & Dooley, S. (2012) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00199.2012 · Cited by 227 · see citing works

Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses

Huebsch, N., Loskill, P., Deveshwar, N., Spencer, C. I., Judge, L. M., Mandegar, M. A., Fox, C. B., Mohamed, T., Ma, Z., Mathur, A., et al. (2016) · Scientific Reports · doi:10.1038/srep24726 · Cited by 227 · see citing works

Modular, pumpless body-on-a-chip platform for the co-culture of GI tract epithelium and 3D primary liver tissue

Esch, M. B., Ueno, H., Applegate, D. R., & Shuler, M. L. (2016) · Lab on a Chip · doi:10.1039/c6lc00461j · Cited by 223 · see citing works

Chip-based human liver–intestine and liver–skin co-cultures – A first step toward systemic repeated dose substance testing in vitro

Maschmeyer, I., Hasenberg, T., Jaenicke, A., Lindner, M., Lorenz, A., Zech, J., Garbe, L., Sonntag, F., Hayden, P. J., Ayehunie, S., et al. (2015) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2015.03.002 · Cited by 220 · see citing works

Systematic reviews of animal models: methodology versus epistemology

Greek, R., & Menache, A. (2013) · International Journal of Medical Sciences · doi:10.7150/ijms.5529 · Cited by 207 · see citing works

A microfluidic approach for in vitro assessment of interorgan interactions in drug metabolism using intestinal and liver slices

van Midwoud, P. M., Merema, M. T., Verpoorte, E., & Groothuis, G. M. M. (2010) · Lab on a Chip · doi:10.1039/c0lc00043d · Cited by 203 · see citing works

Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes

Ware, B. R., Berger, D. R., & Khetani, S. R. (2015) · Toxicological Sciences · doi:10.1093/toxsci/kfv048 · Cited by 196 · see citing works

A modular approach to create a neurovascular unit-on-a-chip

Achyuta, A. K. H., Conway, A. J., Crouse, R. B., Bannister, E. C., Lee, R. N., Katnik, C. P., Behensky, A. A., Cuevas, J., & Sundaram, S. (2012) · Lab on a Chip · doi:10.1039/c2lc41033h · Cited by 191 · see citing works

Afterload promotes maturation of human induced pluripotent stem cell derived cardiomyocytes in engineered heart tissues

Leonard, A., Bertero, A., Powers, J. D., Beussman, K. M., Bhandari, S., Regnier, M., Murry, C. E., & Sniadecki, N. J. (2018) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2018.03.016 · Cited by 191 · see citing works

A 3D microfluidic platform incorporating methacrylated gelatin hydrogels to study physiological cardiovascular cell–cell interactions

Chen, M. B., Srigunapalan, S., Wheeler, A. R., & Simmons, C. A. (2013) · Lab on a Chip · doi:10.1039/c3lc00051f · Cited by 180 · see citing works

Anti-Aβ Drug Screening Platform Using Human iPS Cell-Derived Neurons for the Treatment of Alzheimer's Disease

Yahata, N., Asai, M., Kitaoka, S., Takahashi, K., Asaka, I., Hioki, H., Kaneko, T., Maruyama, K., Saido, T. C., Nakahata, T., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025788 · Cited by 175 · see citing works

Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells

Chen, V. C., Ye, J., Shukla, P., Hua, G., Chen, D., Lin, Z., Liu, J., Chai, J., Gold, J., Wu, J. C., et al. (2015) · Stem Cell Research · doi:10.1016/j.scr.2015.08.002 · Cited by 174 · see citing works

Human-on-chip for therapy development and fundamental science

Luni, C., Serena, E., & Elvassore, N. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.08.015 · Cited by 169 · see citing works

Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system

Guo, X., Gonzalez, M., Stancescu, M., Vandenburgh, H. H., & Hickman, J. J. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.014 · Cited by 168 · see citing works

Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

Mizuno, Y., Chang, H., Umeda, K., Niwa, A., Iwasa, T., Awaya, T., Fukada, S.-I., Yamamoto, H., Yamanaka, S., Nakahata, T., et al. (2010) · The FASEB Journal · doi:10.1096/fj.09-137174 · Cited by 166 · see citing works

Transcriptome Profiling of Patient-Specific Human iPSC-Cardiomyocytes Predicts Individual Drug Safety and Efficacy Responses In Vitro

Matsa, E., Burridge, P. W., Yu, K.-H., Ahrens, J. H., Termglinchan, V., Wu, H., Liu, C., Shukla, P., Sayed, N., Churko, J. M., et al. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.07.006 · Cited by 164 · see citing works

How Drugs are Developed and Approved by the FDA: Current Process and Future Directions

Ciociola, A. A., Cohen, L. B., Kulkarni, P. M., Kefalas, C. H., Buchman, A. L., Burke, C. A., Cain, T., Connor, J., Ehrenpreis, E. D., Fang, J. C., et al. (2014) · The American Journal of Gastroenterology · doi:10.1038/ajg.2013.407 · Cited by 162 · see citing works

Cardiac differentiation of human pluripotent stem cells in scalable suspension culture

Kempf, H., Kropp, C., Olmer, R., Martin, U., & Zweigerdt, R. (2015) · Nature Protocols · doi:10.1038/nprot.2015.089 · Cited by 161 · see citing works

Evaluation of Drugs With Specific Organ Toxicities in Organ-Specific Cell Lines

Lin, Z., & Will, Y. (2011) · Toxicological Sciences · doi:10.1093/toxsci/kfr339 · Cited by 159 · see citing works

How multi-organ microdevices can help foster drug development

Esch, M. B., Smith, A. S. T., Prot, J. M., Oleaga, C., Hickman, J. J., & Shuler, M. L. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.12.003 · Cited by 154 · see citing works

Human induced pluripotent stem cells and their use in drug discovery for toxicity testing

Scott, C. W., Peters, M. F., & Dragan, Y. P. (2013) · Toxicology Letters · doi:10.1016/j.toxlet.2013.02.020 · Cited by 149 · see citing works

Correction: Corrigendum: Decoupling the role of stiffness from other hydroxyapatite signalling cues in periosteal derived stem cell differentiation

Mattei, G., Ferretti, C., Tirella, A., Ahluwalia, A. D., & Mattioli‐Belmonte, M. (2015) · Scientific Reports · doi:10.1038/srep14590 · Cited by 148 · see citing works

Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro

Sarkar, A., Mei, A., Paquola, A. C. M., Stern, S., Bardy, C., Klug, J. R., Kim, S. L., Neshat, N., Kim, H. J., Ku, M., et al. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.04.009 · Cited by 145 · see citing works

Comparison of Human Induced Pluripotent and Embryonic Stem Cells: Fraternal or Identical Twins?

Narsinh, K. H., Plews, J. R., & Wu, J. C. (2011) · Molecular Therapy · doi:10.1038/mt.2011.41 · Cited by 135 · see citing works

Functional differentiation of human pluripotent stem cells on a chip

Giobbe, G. G., Michielin, F., Luni, C., Giulitti, S., Martewicz, S., Dupont, S., Floreani, A., & Elvassore, N. (2015) · Nature Methods · doi:10.1038/nmeth.3411 · Cited by 132 · see citing works

Using physiologically-based pharmacokinetic-guided “body-on-a-chip” systems to predict mammalian response to drug and chemical exposure

Sung, J. H., Srinivasan, B., Esch, M. B., McLamb, W., Bernabini, C., Shuler, M. L., & Hickman, J. J. (2014) · Experimental Biology and Medicine · doi:10.1177/1535370214529397 · Cited by 132 · see citing works

Large-scale generation of human iPSC-derived neural stem cells/early neural progenitor cells and their neuronal differentiation

D’Aiuto, L., Zhi, Y., Kumar Das, D., Wilcox, M. R., Johnson, J. W., McClain, L., MacDonald, M. L., Di Maio, R., Schurdak, M. E., Piazza, P., et al. (2014) · Organogenesis · doi:10.1080/15476278.2015.1011921 · Cited by 131 · see citing works

Myogenic Differentiation of Muscular Dystrophy-Specific Induced Pluripotent Stem Cells for Use in Drug Discovery

Abujarour, R., Bennett, M. H., Valamehr, B. B., Lee, T. T., Robinson, M., Robbins, D., Le, T. M., Lai, K., & Flynn, P. (2014) · Stem Cells Translational Medicine · doi:10.5966/sctm.2013-0095 · Cited by 127 · see citing works

Integrating Human Pluripotent Stem Cells into Drug Development

Engle, S. J., & Puppala, D. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.05.011 · Cited by 126 · see citing works

Effect of Human Donor Cell Source on Differentiation and Function of Cardiac Induced Pluripotent Stem Cells

Sánchez-Freire, V., Lee, A. S., Hu, S., Abilez, O. J., Liang, P., Lan, F., Huber, B. C., Ong, S., Hong, W. X., Huang, M., et al. (2014) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2014.04.056 · Cited by 124 · see citing works

μOrgano: A Lego®-Like Plug & Play System for Modular Multi-Organ-Chips

Loskill, P., Marcus, S. G., Mathur, A., Reese, W. M., & Healy, K. E. (2015) · PLoS ONE · doi:10.1371/journal.pone.0139587 · Cited by 123 · see citing works

Lab-on-a-chip technologies for stem cell analysis

Ertl, P., Sticker, D., Charwat, V., Kasper, C., & Lepperdinger, G. (2014) · Trends in biotechnology · doi:10.1016/j.tibtech.2014.03.004 · Cited by 119 · see citing works

Quantitative Assessment of Population Variability in Hepatic Drug Metabolism Using a Perfused Three-Dimensional Human Liver Microphysiological System

Tsamandouras, N., Kostrzewski, T. M., Stokes, C. L., Griffith, L. G., Hughes, D. J., & Cirit, M. (2016) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.116.237495 · Cited by 119 · see citing works

Stem Cell Culture and Differentiation in Microfluidic Devices Toward Organ-on-a-Chip

Zhang, J., Wei, X., Zeng, R., Xu, F., & Li, X. (2017) · Future Science OA · doi:10.4155/fsoa-2016-0091 · Cited by 115 · see citing works

Controlled electromechanical cell stimulation on-a-chip

Pavesi, A., Adriani, G., Rasponi, M., Zervantonakis, I. K., Fiore, G. B., & Kamm, R. D. (2015) · Scientific Reports · doi:10.1038/srep11800 · Cited by 113 · see citing works

Metabolic Profiling of Human Long-Term Liver Models and Hepatic Clearance Predictions from In Vitro Data Using Nonlinear Mixed-Effects Modeling

Kratochwil, N. A., Meille, C., Fowler, S. M., Klammers, F., Ekiciler, A., Molitor, B., Simon, S., Walter, I., McGinnis, C., Walther, J., et al. (2017) · The AAPS Journal · doi:10.1208/s12248-016-0019-7 · Cited by 111 · see citing works

Generation of Human iPSCs from Human Peripheral Blood Mononuclear Cells Using Non-integrative Sendai Virus in Chemically Defined Conditions

Churko, J. M., Burridge, P. W., & Wu, J. C. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-511-8_7 · Cited by 95 · see citing works

Prediction of human population responses to toxic compounds by a collaborative competition

Eduati, F., Mangravite, L. M., Wang, T., Tang, H., Bare, J. C., Huang, R., Norman, T., Kellen, M., Menden, M. P., Yang, J., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3299 · Cited by 95 · see citing works

Prediction of Human Population Responses to Toxic Compounds by a Collaborative Competition

Bynagari, N. B. (2017) · Asian Journal of Humanity Art and Literature · doi:10.18034/ajhal.v4i2.577 · Cited by 91 · see citing works

Controlling Differentiation of Stem Cells for Developing Personalized Organ‐on‐Chip Platforms

Geraili, A., Jafari, P., Hassani, M. S., Araghi, B. H., Mohammadi, M. H., Ghafari, A. M., Tamrin, S. H., Modarres, H. P., Kolahchi, A. R., Ahadian, S., et al. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700426 · Cited by 86 · see citing works

Niemann–Pick Disease Type C: Induced Pluripotent Stem Cell–Derived Neuronal Cells for Modeling Neural Disease and Evaluating Drug Efficacy

Yu, D., Swaroop, M., Wang, M., Baxa, U., Yang, R., Yan, Y., Coksaygan, T., DeTolla, L. J., Marugán, J., Austin, C. P., et al. (2014) · SLAS DISCOVERY · doi:10.1177/1087057114537378 · Cited by 83 · see citing works

Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs

Zhao, M., Chen, H., Liu, Q., Shao, N., Sayed, N., Wo, H.-T., Zhang, J. Z., Ong, S., Liu, C., Kim, Y., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1708991114 · Cited by 78 · see citing works

Comparative Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells Reveals Current Drawbacks and Possible Strategies for Improved Differentiation

Józefczuk, J., Prigione, A., Chávez, L., & Adjaye, J. (2010) · Stem Cells and Development · doi:10.1089/scd.2010.0361 · Cited by 77 · see citing works

Global transcriptional profiling reveals similarities and differences between human stem cell-derived cardiomyocyte clusters and heart tissue

Synnergren, J., Améen, C., Jansson, A., & Sartipy, P. (2011) · Physiological Genomics · doi:10.1152/physiolgenomics.00118.2011 · Cited by 72 · see citing works

A human iPS cell myogenic differentiation system permitting high-throughput drug screening

Uchimura, T., Otomo, J., Sato, M., & Sakurai, H. (2017) · Stem Cell Research · doi:10.1016/j.scr.2017.10.023 · Cited by 70 · see citing works

A 3D toolbox to enhance physiological relevance of human tissue models

Picollet-D’hahan, N., Dolega, M. E., Liguori, L., Marquette, C., Le Gac, S., Gidrol, X., & Martin, D. K. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.06.012 · Cited by 65 · see citing works

Integration ofin silicoandin vitroplatforms for pharmacokinetic–pharmacodynamic modeling

Sung, J. H., Esch, M. B., & Shuler, M. L. (2010) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2010.496251 · Cited by 64 · see citing works

3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling

Li, X., Brooks, J. C., Hu, J., Ford, K. I., & Easley, C. J. (2016) · Lab on a Chip · doi:10.1039/c6lc01201a · Cited by 63 · see citing works

Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity

Choudhury, Y., Toh, Y., Xing, J., Qu, Y., Poh, J., Li, H., Tan, H. S., Kanesvaran, R., Yu, H., & Tan, M. (2017) · Scientific Reports · doi:10.1038/srep41238 · Cited by 61 · see citing works

Human pluripotent stem cell-derived cardiomyocytes: Genome-wide expression profiling of long-term in vitro maturation in comparison to human heart tissue

Piccini, I., Rao, J., Seebohm, G., & Greber, B. (2015) · Genomics Data · doi:10.1016/j.gdata.2015.03.008 · Cited by 60 · see citing works

Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs

Ramsden, C. M., Nommiste, B., Lane, A., Carr, A. F., Powner, M. B., Smart, M. J. K., Chen, L., Muthiah, M. N., Webster, A. R., Moore, A. T., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-00142-7 · Cited by 53 · see citing works

Toxicity testing and drug screening using iPSC-derived hepatocytes, cardiomyocytes, and neural cells

Csöbönyeiová, M., Polák, Š., & Danišovič, Ľ. (2016) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/cjpp-2015-0459 · Cited by 52 · see citing works

Transcriptomic and epigenomic differences in human induced pluripotent stem cells generated from six reprogramming methods

Churko, J. M., Lee, J., Ameen, M., Gu, M., Venkatasubramanian, M., Diecke, S., Sallam, K., Im, H., Wang, G., Gold, J. D., et al. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0141-6 · Cited by 51 · see citing works

Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions

Yoshimitsu, R., Hattori, K., Sugiura, S., Kondo, Y., Yamada, R., Tachikawa, S., Satoh, T., Kurisaki, A., Ohnuma, K., Asashima, M., et al. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.25150 · Cited by 43 · see citing works

A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells

Hesari, Z., Soleimani, M., Atyabi, F., Sharifdini, M., Nadri, S., Warkiani, M. E., Zare, M., & Dinarvand, R. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35689 · Cited by 34 · see citing works

Opportunities and challenges in the wider adoption of liver and interconnected microphysiological systems

Hughes, D. J., Kostrzewski, T. M., & Sceats, E. L. (2017) · Experimental Biology and Medicine · doi:10.1177/1535370217708976 · Cited by 31 · see citing works

Fusing Tissue Engineering and Systems Biology Toward Fulfilling Their Promise

Cosgrove, B. D., Griffith, L. G., & Lauffenburger, D. A. (2008) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-008-0007-9 · Cited by 25 · see citing works

Molecular Pathways Governing Development of Vascular Endothelial Cells from ES/iPS Cells

Tan, K. S., Tamura, K., Lai, M. I., Veerakumarasivam, A., Nakanishi, Y., Ogawa, M., & Sugiyama, D. (2013) · Stem Cell Reviews and Reports · doi:10.1007/s12015-013-9450-7 · Cited by 25 · see citing works

Organ-on-a-chip: recent breakthroughs and future prospects

Wu, Q., Liu, J., Wang, X., Feng, L., Wu, J., Zhu, X., Wen, W., & Gong, X. (2020) · BioMedical Engineering OnLine · doi:10.1186/s12938-020-0752-0 · Cited by 704 · see citing works

Patient and Disease–Specific Induced Pluripotent Stem Cells for Discovery of Personalized Cardiovascular Drugs and Therapeutics

Paik, D. T., Chandy, M., & Wu, J. C. (2019) · Pharmacological Reviews · doi:10.1124/pr.116.013003 · Cited by 216 · see citing works

Use of Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes in Preclinical Cancer Drug Cardiotoxicity Testing: A Scientific Statement From the American Heart Association

Gintant, G. A., Burridge, P. W., Gepstein, L., Harding, S. E., Herron, T. J., Hong, C. C., Jalife, J., & Wu, J. C. (2019) · Circulation Research · doi:10.1161/res.0000000000000291 · Cited by 160 · see citing works

Progress in multicellular human cardiac organoids for clinical applications

Kim, H., Kamm, R. D., Vunjak‐Novakovic, G., & Wu, J. C. (2022) · Cell stem cell · doi:10.1016/j.stem.2022.03.012 · Cited by 153 · see citing works

Kidney-based in vitro models for drug-induced toxicity testing

Faria, J., Ahmed, S., Gerritsen, K. G. F., Mihaila, S. M., & Masereeuw, R. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02598-0 · Cited by 152 · see citing works

Emerging trends in organ-on-a-chip systems for drug screening

Wang, Y., Gao, Y., Pan, Y., Zhou, D., Liu, Y., Yin, Y. B., Yang, J., Wang, Y., & Song, Y. (2023) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2023.02.006 · Cited by 130 · see citing works

Recent progress in translational engineered in vitro models of the central nervous system

Nikolakopoulou, P., Rauti, R., Voulgaris, D., Shlomy, I., Maoz, B. M., & Herland, A. (2020) · Brain · doi:10.1093/brain/awaa268 · Cited by 128 · see citing works

Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function

Wnorowski, A. N., Sharma, A., Chen, H., Wu, H., Shao, N., Sayed, N., Liu, C., Countryman, S. M., Stodieck, L., Rubins, K., et al. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.10.006 · Cited by 116 · see citing works

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Dellaquila, A., Le Bao, C., Letourneur, D., & Simon‐Yarza, T. (2021) · Advanced Science · doi:10.1002/advs.202100798 · Cited by 115 · see citing works

3D bioprinted organ‐on‐chips

Dabbagh, S. R., Sarabi, M. R., Birtek, M. T., Mustafaoğlu, N., Zhang, Y. S., & Taşoğlu, S. (2022) · Aggregate · doi:10.1002/agt2.197 · Cited by 110 · see citing works

Breakthroughs and Applications of Organ-on-a-Chip Technology

Koyilot, M. C., Natarajan, P., Hunt, C. R., Sivarajkumar, S., Roy, R., Joglekar, S. S., Pandita, S., Tong, C. W.-C., Marakkar, S., Subramanian, L., et al. (2022) · Cells · doi:10.3390/cells11111828 · Cited by 110 · see citing works

Recent advances in lung-on-a-chip models

Francis, I., Shrestha, J., Paudel, K. R., Hansbro, P. M., Warkiani, M. E., & Saha, S. C. (2022) · Drug Discovery Today · doi:10.1016/j.drudis.2022.06.004 · Cited by 105 · see citing works

Generation of Endothelial Cells From Human Pluripotent Stem Cells

Williams, I. M., & Wu, J. C. (2019) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.119.312265 · Cited by 93 · see citing works

Future Perspective of Diabetic Animal Models

Pandey, S., & Dvorakova, M. C. (2019) · Endocrine Metabolic & Immune Disorders - Drug Targets · doi:10.2174/1871530319666190626143832 · Cited by 92 · see citing works

Translational Roadmap for the Organs-on-a-Chip Industry toward Broad Adoption

Allwardt, V., Ainscough, A. J., Viswanathan, P., Sherrod, S. D., MCLEAN, J. A. J., Haddrick, M., & Pensabene, V. (2020) · Bioengineering · doi:10.3390/bioengineering7030112 · Cited by 89 · see citing works

Microfluidics for Neuronal Cell and Circuit Engineering

Habibey, R., Arias, J. E. R., Striebel, J., & Busskamp, V. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.2c00212 · Cited by 86 · see citing works

Reconstructing the heart using iPSCs: Engineering strategies and applications

Cho, S., Lee, C., Skylar‐Scott, M. A., Heilshorn, S. C., & Wu, J. C. (2021) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2021.04.006 · Cited by 74 · see citing works

Multifunctional microfluidic chip for cancer diagnosis and treatment

Guo, Q., Zhang, L.-L., Liu, J., Li, Z., Li, J.-J., Zhou, W., Wang, H., Li, J., Liu, D., Yu, X., et al. (2020) · Nanotheranostics · doi:10.7150/ntno.49614 · Cited by 74 · see citing works

Revolutionizing immune research with organoid-based co-culture and chip systems

Papp, D., Korcsmáros, T., & Hautefort, I. (2024) · Clinical & Experimental Immunology · doi:10.1093/cei/uxae004 · Cited by 73 · see citing works

Implementing organ-on-chip in a next-generation risk assessment of chemicals: a review

Nitsche, K. S., Müller, I. I., Malcomber, S., Carmichael, P. L., & Bouwmeester, H. (2022) · Archives of Toxicology · doi:10.1007/s00204-022-03234-0 · Cited by 66 · see citing works

Human Organoids for the Study of Retinal Development and Disease

Bell, C. M., Zack, D. J., & Berlinicke, C. A. (2020) · Annual Review of Vision Science · doi:10.1146/annurev-vision-121219-081855 · Cited by 63 · see citing works

Engineering Biomaterials with Micro/Nanotechnologies for Cell Reprogramming

Fang, J., Hsueh, Y., Soto, J., Sun, W., Wang, J., Gu, Z., Khademhosseini, A., & Li, S. (2020) · ACS Nano · doi:10.1021/acsnano.9b04837 · Cited by 60 · see citing works

Precision Oncology Beyond Genomics: The Future Is Here—It Is Just Not Evenly Distributed

Pfohl, U., Pflaume, A., Regenbrecht, M., Finkler, S., Adelmann, Q. G., Reinhard, C., Regenbrecht, C. R. A., & Wedeken, L. (2021) · Cells · doi:10.3390/cells10040928 · Cited by 60 · see citing works

Potential applications of microfluidics based blood brain barrier (BBB)-on-chips for in vitro drug development

Wang, X., Hou, Y., Ai, X., Sun, J., Xu, B., Meng, X., Zhang, Y., & Zhang, S. (2020) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.110822 · Cited by 58 · see citing works

Brain-on-a-Chip: Characterizing the next generation of advanced in vitro platforms for modeling the central nervous system

Maoz, B. M. (2021) · APL Bioengineering · doi:10.1063/5.0055812 · Cited by 57 · see citing works

Recent advances in 3D-printing-based organ-on-a-chip

Wu, X., Shi, W., Liu, X., & Gu, Z. (2024) · EngMedicine · doi:10.1016/j.engmed.2024.100003 · Cited by 57 · see citing works

Developer’s Guide to an Organ-on-Chip Model

Rogal, J., Schlünder, K., & Loskill, P. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c01536 · Cited by 54 · see citing works

Human Organs‐on‐Chips: A Review of the State‐of‐the‐Art, Current Prospects, and Future Challenges

Zarrintaj, P., Saeb, M. R., Stadler, F. J., Yazdi, M. K., Nasirinezhad, M., Mohebbi, S., Seidi, F., Saeb, M. R., & Mozafari, M. (2021) · Advanced Biology · doi:10.1002/adbi.202000526 · Cited by 48 · see citing works

Advanced Fabrication Techniques of Microengineered Physiological Systems

Puryear, J. R., Yoon, J., & Kim, Y. (2020) · Micromachines · doi:10.3390/mi11080730 · Cited by 48 · see citing works

Reshaping transplantation with AI, emerging technologies and xenotransplantation

Loupy, A., Preka, E., Chen, X., Wang, H., He, J., & Zhang, K. (2025) · Nature Medicine · doi:10.1038/s41591-025-03801-9 · Cited by 50 · see citing works

Cancer Models on Chip: Paving the Way to Large‐Scale Trial Applications

Gil, J. F., Moura, C. S., Silvério, V., Gonçalves, G., & Santos, H. A. (2023) · Advanced Materials · doi:10.1002/adma.202300692 · Cited by 45 · see citing works

Assessment of doxorubicin toxicity using human cardiac organoids: A novel model for evaluating drug cardiotoxicity

Chen, X., Lu, N., Huang, S., Zhang, Y., Liu, Z., & Wang, X. (2023) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2023.110777 · Cited by 47 · see citing works

Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

Zheng, F., Tian, R., Lu, H., Xiao, L., Shafiq, M., Uchida, S., Chen, H., & Ma, M. (2024) · Small · doi:10.1002/smll.202401400 · Cited by 44 · see citing works

Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts

Rosner, A., Armengaud, J., Ballarin, L., Barnay‐Verdier, S., Cima, F., Coelho, A. V., Domart-Coulon, I. J., Drobne, D., Genevière, A., Kokalj, A. J., et al. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.144565 · Cited by 43 · see citing works

Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin

Baddal, B., & Marrazzo, P. (2021) · Pathogens · doi:10.3390/pathogens10020203 · Cited by 38 · see citing works

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

Zommiti, M., Connil, N., Tahrioui, A., Groboillot, A., Barbey, C., Konto‐Ghiorghi, Y., Lesouhaitier, O., Chevalier, S., & Feuilloley, M. G. J. (2022) · Bioengineering · doi:10.3390/bioengineering9110646 · Cited by 39 · see citing works

Glioma-on-a-Chip Models

Ustun, M., Dabbagh, S. R., Ilci, I., Onder, T. B., & Taşoğlu, S. (2021) · Micromachines · doi:10.3390/mi12050490 · Cited by 37 · see citing works

Clinical Trial in a Dish

Lam, C. K., & Wu, J. C. (2021) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.120.314695 · Cited by 37 · see citing works

Skin-on-a-chip technologies towards clinical translation and commercialization

Ismayilzada, N., Tarar, C., Dabbagh, S. R., Tokyay, B. K., Dilmani, S. A., Sokullu, E., Abaci, H. E., & Taşoğlu, S. (2024) · Biofabrication · doi:10.1088/1758-5090/ad5f55 · Cited by 37 · see citing works

The Growing Importance of Three-Dimensional Models and Microphysiological Systems in the Assessment of Mycotoxin Toxicity

Zingales, V., Esposito, M. R., Torriero, N., Taroncher, M., Cimetta, E., & Ruiz, M.-J. (2023) · Toxins · doi:10.3390/toxins15070422 · Cited by 33 · see citing works

Organoid-on-a-chip: Current challenges, trends, and future scope toward medicine

Li, Z., Li, Q., Zhou, C., Lu, K., Liu, Y., Xuan, L., & Wang, X. (2023) · Biomicrofluidics · doi:10.1063/5.0171350 · Cited by 33 · see citing works

Blood–brain barrier microfluidic chips and their applications

Li, M., Zhu, M., Huang, R., Wang, K., Zeng, Z., Xiao, L. X., Lin, Y., & Liu, D. (2023) · Organs-on-a-Chip · doi:10.1016/j.ooc.2023.100027 · Cited by 30 · see citing works

Organ-on-a-Chip systems for new drugs development

Vargas, R., Medina, L., & Egurbide, A. (2021) · ADMET & DMPK · doi:10.5599/admet.942 · Cited by 30 · see citing works

Modelling myocardial ischemia/reperfusion injury with inflammatory response in human ventricular cardiac organoids

Zhang, L., Jiang, Y. Y., Jia, W., Le, W., Liu, J., Zhang, P., Yang, H., Liu, Z., & Liu, Y. (2024) · Cell Proliferation · doi:10.1111/cpr.13762 · Cited by 30 · see citing works

Organ-on-a-Chip Models—New Possibilities in Experimental Science and Disease Modeling

Wysoczański, B., Świątek, M., & Wójcik-Gładysz, A. (2024) · Biomolecules · doi:10.3390/biom14121569 · Cited by 26 · see citing works

Emergent Applications of Organ-on-a-Chip (OOAC) Technologies With Artificial Vascular Networks in the 21st Century

Barua, D. R., Biswas, N., & Das, D. (2024) · Advances in healthcare information systems and administration book series · doi:10.4018/979-8-3693-1214-8.ch010 · Cited by 24 · see citing works

Unraveling Muscle Impairment Associated With COVID-19 and the Role of 3D Culture in Its Investigation

Seixas, M. L., Mitre, L. P., Shams, S., Lanzuolo, G. B., Bartolomeo, C. S., Silva, E. A., Prado, C. M., Ureshino, R. P., & Stilhano, R. S. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.825629 · Cited by 24 · see citing works

Bioethical implications of organ‐on‐a‐chip on modernizing drug development

Thakar, R. G., & Fenton, K. N. (2023) · Artificial Organs · doi:10.1111/aor.14620 · Cited by 22 · see citing works

Potential of Drug Efficacy Evaluation in Lung and Kidney Cancer Models Using Organ-on-a-Chip Technology

Hwang, S.-H., Lee, S., Park, J. Y., Jeon, J. S., Cho, Y., & Kim, S. (2021) · Micromachines · doi:10.3390/mi12020215 · Cited by 22 · see citing works

The toxicity and safety of Chinese medicine from the bench to the bedside

Zhu, H., Wang, X., Wang, X., Pan, G., Zhu, Y., & Feng, Y. (2021) · Journal of Herbal Medicine · doi:10.1016/j.hermed.2021.100450 · Cited by 21 · see citing works

Trends in organ-on-a-chip for pharmacological analysis

Xu, X., Cheung, S., Jia, X., Fan, G., Ai, Y., Zhang, Y., & Liang, Q. (2024) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2024.117905 · Cited by 20 · see citing works

Organ-on-a-chip and 3D printing as preclinical models for medical research and practice

Jain, A., Mathur, T., Pandian, N. K. R., & Selahi, A. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-819178-1.00009-5 · Cited by 20 · see citing works

Improving the engraftment and integration of cell transplantation for cardiac regeneration

Tu, C., Mezynski, R., & Wu, J. C. (2019) · Cardiovascular Research · doi:10.1093/cvr/cvz237 · Cited by 20 · see citing works

New Endeavors of (Micro)Tissue Engineering: Cells Tissues Organs on-Chip and Communication Thereof

Ahmed, H. M. M., & Teixeira, L. M. (2021) · Cells Tissues Organs · doi:10.1159/000516356 · Cited by 18 · see citing works

Human Induced Pluripotent Stem Cells as a Screening Platform for Drug-Induced Vascular Toxicity

Tu, C., Cunningham, N. J., Zhang, M., & Wu, J. C. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.613837 · Cited by 18 · see citing works

Development of Organs-on-Chips and Their Impact on Precision Medicine and Advanced System Simulation

Luo, Y., Li, X., Zhao, Y., Zhong, W., Xing, M. M. Q., & Lyu, G. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15082094 · Cited by 17 · see citing works

Lung-on-chip microdevices to foster pulmonary drug discovery

Sisodia, Y., Shah, K., Sayyed, A. A., Jain, M., Ali, S. A., Gondaliya, P., Kalia, K., & Tekade, R. K. (2022) · Biomaterials Science · doi:10.1039/d2bm00951j · Cited by 17 · see citing works

Organoids: A new frontier in precision medicine and engineering

Gao, Q., Wu, Y., Mao, Y., Chen, X., Geng, Z., & Su, J. (2025) · Precision medicine and engineering. · doi:10.1016/j.preme.2025.100049 · Cited by 17 · see citing works

Building Blood Vessel Chips with Enhanced Physiological Relevance

Mu, X., Gerhard‐Herman, M. D., & Zhang, Y. S. (2023) · Advanced Materials Technologies · doi:10.1002/admt.202201778 · Cited by 14 · see citing works

Multistep Fluidic Control Network toward the Automated Generation of Organ-on-a-Chip

Hsieh, H.-L., Nath, P., & Huang, J. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00912 · Cited by 14 · see citing works

Bioprinting approaches in cardiac tissue engineering to reproduce blood-pumping heart function

Kim, M. J., Hwang, D. G., & Jang, J. (2024) · iScience · doi:10.1016/j.isci.2024.111664 · Cited by 12 · see citing works

Modeling diabetic cardiomyopathy using human cardiac organoids: Effects of high glucose and lipid conditions

Wang, X., Tan, X., Zhang, T., Xu, S., Zeng, Y., Xu, A., Li, X., Zhang, G., Jiang, Y., Jiang, H., et al. (2025) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2025.111421 · Cited by 12 · see citing works

Integrating Biophysics in Toxicology

Favero, G. D., & Kraegeloh, A. (2020) · Cells · doi:10.3390/cells9051282 · Cited by 12 · see citing works

Characterization and Applicability of a Bone Spheroid Model for the Evaluation of Cytocompatibility of Bone Substitutes

Brochado, A. C. B., Costa-Silva, D., da Silva, J. C., Lowenstein, A., Gameiro, V. S., Mavropoulos, E., Mourão, C. F., & Alves, G. G. (2023) · Applied Sciences · doi:10.3390/app13031602 · Cited by 11 · see citing works

(Bio)fabrication of microfluidic devices and organs-on-a-chip

Hrynevich, A., Li, Y., Cedillo‐Servín, G., Malda, J., & Castilho, M. D. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-89831-7.00001-8 · Cited by 11 · see citing works

Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips

Lecoq, P.-E., Dupuis, C., Mousset, X., Benoit-Gonin, X., Peyrin, J., & Aider, J. (2024) · npj Microgravity · doi:10.1038/s41526-024-00355-x · Cited by 11 · see citing works

Biomimetic microfluidic chips for toxicity assessment of environmental pollutants

Du, X.-Y., & Yang, J. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.170745 · Cited by 11 · see citing works

Replicating Host–Microbiome Interactions: Harnessing Organ-on-a-Chip and Organoid Technologies to Model Vaginal and Lung Physiology

Coxon, J., Linder, E., Sweet, C. W., Magness, S. T., & Green, L. N. (2025) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-110122-122343 · Cited by 12 · see citing works

Molecular and epigenetic alterations in normal and malignant myelopoiesis in human leukemia 60 (HL60) promyelocytic cell line model

Basu, J., Madhulika, S., Murmu, K. C., Mohanty, S., Samal, P., Das, A., Mahapatra, S., Saha, S., Sinha, I., & Prasad, P. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1060537 · Cited by 11 · see citing works

Deconvoluting the Cells of the Human Heart with iPSC Technology: Cell Types, Protocols, and Uses

Yu, B., Zhao, S. R., Yan, C. D., Zhang, M., & Wu, J. C. (2022) · Current Cardiology Reports · doi:10.1007/s11886-022-01670-z · Cited by 11 · see citing works

Applications, challenges, and prospects of induced pluripotent stem cells for vascular disease

Biswas, P. K., & Park, J. (2024) · Molecules and Cells · doi:10.1016/j.mocell.2024.100077 · Cited by 10 · see citing works

Synergistic potential of stem cells and microfluidics in regenerative medicine

Rajalekshmi, R., & Agrawal, D. K. (2024) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-024-05108-8 · Cited by 10 · see citing works

A comprehensive review on organ-on-chips as powerful preclinical models to study tissue barriers

Filiz, Y., Esposito, A., Maria, C. D., Vozzi, G., & Yeşil-Çeliktaş, Ö. (2024) · Progress in Biomedical Engineering · doi:10.1088/2516-1091/ad776c · Cited by 12 · see citing works

The Implementation of the Three Rs in Regulatory Toxicity and Biosafety Assessment: The Indian Perspective

Pant, A. B. (2020) · Alternatives to Laboratory Animals · doi:10.1177/0261192920986811 · Cited by 8 · see citing works

Organ-on-a-chip toxicology

Yang, S., Zhang, T., Ge, Y., Niu, Y., Chen, M., Yin, L., Pu, Y., Chen, Z., Gu, Z., & Liang, G. (2026) · The Innovation · doi:10.1016/j.xinn.2025.101252 · Cited by 7 · see citing works

Engineering HepG2 spheroids with injectable fiber fragments as predictable models for drug metabolism and tumor infiltration

Wei, J., Lei, D., Chen, M., Ran, P., & Li, X. (2020) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34669 · Cited by 7 · see citing works

State of the Art in Integrated Biosensors for Organ-on-a-Chip Applications

Dey, T., Mitra, P., Chakraborty, B., Sanyal, A., Acharjee, A., Ghosh, A., & Mandal, D. (2024) · Smart nanomaterials technology · doi:10.1007/978-981-99-6597-7_10 · Cited by 7 · see citing works

Multiorgan-on-a-chip: an advanced platform for disease modeling, drug toxicity assessment, and therapeutic screening

Qiao, L., Chang, S., Zou, L., Zhang, F., Cui, C., & Huang, N. (2025) · Bio-Design and Manufacturing · doi:10.1631/bdm.2500076 · Cited by 6 · see citing works

Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days

Won, N., Castillo-Prado, J., Tan, X., Ford, J., Heath, D., Mazilescu, L., Selzner, M., & Rogers, I. M. (2022) · Cells · doi:10.3390/cells11111822 · Cited by 6 · see citing works

Fasudil and viscosity of gelatin promote hepatic differentiation by regulating organelles in human umbilical cord matrix-mesenchymal stem cells

Choi, J., Kang, S., An, H., Kim, C.-E., Lee, S. H., Pack, C., Yoon, Y., Jin, H., Cho, Y., Kim, C. J., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03851-9 · Cited by 5 · see citing works

Bridging the Gap: How Organ‐on‐a‐Chip Technology Facilitates the Battle against Glioma

Liu, S., Gong, Z., Zhou, D., Ayala‐Nunez, V., Xu, T., & Wick, P. (2025) · Small Science · doi:10.1002/smsc.202500154 · Cited by 5 · see citing works

Dual-cell culture system with identical culture environment for comparison of anti-cancer drug toxicity

Lai, W.-H., Mu, H., Lu, Y., Chen, H., Wen, J.-W., Wu, H., Cheng, C., & Huang, J. (2022) · Chemical Engineering Science · doi:10.1016/j.ces.2022.117555 · Cited by 5 · see citing works

3D Bioprinting of Collagen-based Microfluidics for Engineering Fully-biologic Tissue SystemsPreprint

Shiwarski, D. J., Hudson, A. R., Tashman, J. W., Bakırcı, E., Moss, S., Coffin, B. D., & Feinberg, A. W. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.26.577422 · Cited by 5 · see citing works

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNFα-promotes severe intestinal epithelial dysfunction

Dutton, J. S., Hinman, S. S., Kim, R., Attayek, P. J., Maurer, M. A., Sims, C. E., & Allbritton, N. L. (2021) · Integrative Biology · doi:10.1093/intbio/zyab008 · Cited by 4 · see citing works

Recent Updates on Metal-Polymer Nanocomposites in 3D Bioprinting for Tissue Engineering Applications

Flora, B., Kumar, R., Mahdieh, R., Zarei, K., Chehrazi, S., Kaur, S. D., Sharma, A., Mohapatra, P., Sakshi, A., Singh, A., et al. (2023) · Nanofabrication · doi:10.37819/nanofab.008.291 · Cited by 4 · see citing works

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Nelson, E. A., Qiu, J., Chavkin, N. W., & Hirschi, K. K. (2021) · Journal of Visualized Experiments · doi:10.3791/62391 · Cited by 4 · see citing works

Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Brochado, A. C. B., de Souza, V. H., Correa, J., dos Anjos, S. A., Mourão, C. F., Cardarelli, A., Montemezzi, P., Gameiro, V. S., Pereira, M. R., Mavropoulos, E., et al. (2021) · Materials · doi:10.3390/ma14195858 · Cited by 4 · see citing works

Organ-on-a-Chip and Lab-on-a-Chip Technologies in Cardiac Tissue Engineering

Marazzi, D., Trovalusci, F., Nardo, P. D., & Carotenuto, F. (2025) · Biomimetics · doi:10.3390/biomimetics11010018 · Cited by 3 · see citing works

Investigational New Drug ‐enabling studies in a human vessel‐chip: Are we there yet?

Kumar, A., Maringanti, R., Mathur, T., Abe, J., Le, N., Xiao, Y., Wang, G., Mojiri, A., Cooke, J. P., & Jain, A. (2026) · Bioengineering & Translational Medicine · doi:10.1002/btm2.70129 · Cited by 4 · see citing works

Adaptation of the Kirkstall QV600 LLI Microfluidics System for the Study of Gastrointestinal Absorption by Mass Spectrometry Imaging and LC-MS/MS

Spencer, C. E., Rumbelow, S. J., Mellor, S., Duckett, C. J., & Clench, M. R. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020364 · Cited by 3 · see citing works

Efforts to evaluate translational science’s impact on biomedicine and society should incorporate Science and Technology Studies (STS)

Molldrem, S., Moses, J. D., Tumilty, E., Swanson, P., Farroni, J. S., & Smith, E. M. R. (2025) · Journal of Clinical and Translational Science · doi:10.1017/cts.2025.10120 · Cited by 2 · see citing works

Cell‐embedded microgels as emerging miniature 3D tissue‐mimics toward biochip‐based toxicity screening

Delafosse, M., Regnault, E., Gebauer‐Barrett, J., Manz, A., & Sung, B. (2025) · Bioengineering & Translational Medicine · doi:10.1002/btm2.70061 · Cited by 2 · see citing works

Application and prospects of lung organ-on-a-chip in the development of new drugs

Su, J., Qiu, J., Meng, J., Gong, Y., Xu, Y., & Han, J. (2025) · BioMedical Engineering OnLine · doi:10.1186/s12938-025-01497-z · Cited by 2 · see citing works

Infrapatellar Fat Pad-Derived Non-Cellular Products in Therapy for Musculoskeletal Diseases: A Scoping Review

Triangga, A. F. R., Asmara, W., Magetsari, R., Bachtiar, I., Fazatamma, D. A., Saraswati, P. A., Huwaidi, A. F., & Wirohadidjojo, Y. W. (2024) · Orthopedic Reviews · doi:10.52965/001c.125841 · Cited by 2 · see citing works

Isogenic hiPSC-derived liver-on-chip platforms: A valuable tool for modeling metabolic liver diseases

Aina, K. O. (2023) · Aspects of Molecular Medicine · doi:10.1016/j.amolm.2023.100025 · Cited by 2 · see citing works

Development of Human-on-a-Chip

Jacob, E. M., Borah, A., & Kumar, D. S. (2022) · Springer eBooks · doi:10.1007/978-981-19-1379-2_12 · Cited by 2 · see citing works

Stem cell based human organ-on-a-chip models for drug discovery and development

Loskill, P., & Wu, J. C. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.07.004 · Cited by 2 · see citing works

Organ-on-Chip: The Advancing Microfluidic Technology for Theranostics

Raheem, A. (2024) · Premier journal of science. · doi:10.70389/pjs.100029 · Cited by 1 · see citing works

Development of a vascularized multi-organoid-on-a-chip to model the heart-islet axis in diabetic cardiomyopathy

Yang, Z., Lin, L., Jiang, J., Wang, Y., Li, F., Li, X., Fang, J., Xu, L., Li, H., Du, L., et al. (2026) · Bioactive Materials · doi:10.1016/j.bioactmat.2026.05.033 · Cited by 1 · see citing works

hiPSC disease modeling with 3D organoids

Cunningham, N. J., Bhoi, R., & Yang, H. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-99892-5.00021-9 · Cited by 1 · see citing works

Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development

Ronaldson-Bouchard, K., & Vunjak-Novakovic, G. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.02.011 · Cited by 657 · see citing works

Organs-on-a-chip in drug development

Mohandas, S., Kumaran, K., & Ramkumar, K. M. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13782-2.00001-2

Controlling Differentiation of Stem Cells for Developing Personalized Organ‐on‐Chip Platforms

Geraili, A., Jafari, P., Hassani, M. S., Araghi, B. H., Mohammadi, M. H., Ghafari, A. M., Tamrin, S. H., Modarres, H. P., Kolahchi, A. R., Ahadian, S., et al. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700426 · Cited by 86 · see citing works

Stem cell based human organ-on-a-chip models for drug discovery and development

Loskill, P., & Wu, J. C. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.07.004 · Cited by 2 · see citing works

Organs-on-chip: The way forward

Mastrangeli, M., & Raaij, J. V. D. E. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.06.015 · Cited by 61 · see citing works