A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs
Open accessAbstract
Anti-angiogenic therapy is now widely accepted as a treatment for cancer. We introduce a new microfluidic platform that can monitor and quantify cellular behaviors, such as morphological changes, endothelial cell viability, and formation of angiogenic sprouts, depending on the various concentrations of drug applied (i.e., bortezomib, a selective 26S proteasome inhibitor).
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Fibroblasts can boost angiogenesis in in vitro assays.
“fibroblasts can boost angiogenesis in in vitro assays, with exciting advances in the development of microfluidic angiogenesis models, and an increasing number of studies have shown how they can alter T cell functionality118,161,162.”
From A framework for advancing our understanding of cancer-associated fibroblasts (2020) · cites this paper as [161]
The Results and Discussion section (‘High throughput angiogenesis assay’; page unavailable) reports increased endothelial migration and lumen-like growth in response to VEGF, not fibroblasts. Searches for ‘fibroblast’ and fibroblast-conditioned media found no passage in our copy addressing whether fibroblasts boost angiogenesis in vitro. (LLM, conf=0.76)
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Analyzing angiogenesis on a chip using deep learning-based image processing
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Biomimetic tumor-induced angiogenesis and anti-angiogenic therapy in a microfluidic model
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Engineered Microphysiological Systems for Testing Effectiveness of Cell-Based Cancer Immunotherapies
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Microfluidics for Angiogenesis Research
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- Authors
- Kim, C., Kasuya, J., Jeon, J., Chung, S., & Kamm, R. D.
- Affiliations
- Kyungil University (KR)
- Massachusetts Institute of Technology (US)
- Korea University (KR)
- Singapore-MIT Alliance for Research and Technology (SG)
- Corresponding author
- Kamm, Roger D.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2015
- Volume
- 15
- Issue
- 1
- Pages
- 301-310
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC4311754
- OpenAlex
- W2083166046
- Cited by
- 143
- References
- 39
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 11 · 2023: 10 · 2022: 9 · 2021: 20 · 2020: 21 · 2019: 18 · 2018: 10 · 2017: 11 · 2016: 11 · 2015: 9 · 2014: 1
- FWCI
- 4.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
- PMC
- Funders
- National Cancer Institute (R33CA174550)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- angiogenesis inhibitors, angiogenesis, high-throughput screening, human umbilical vein endothelial cells, bortezomib, proteasome inhibitors, endothelial cell death, tumor hypoxia
- MeSH terms
- Bortezomib, Boronic Acids, Cell Movement, Cell Survival, Humans, Neovascularization, Pathologic, Pyrazines, Cell Culture Techniques, Angiogenesis Inhibitors, Vascular Endothelial Growth Factor A, Microfluidic Analytical Techniques, Cell Proliferation, Human Umbilical Vein Endothelial Cells
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