The DOI matches its Crossref record; 83 of the 83 references it cites were found in a registry or an index.
Cytochrome P450 Monooxygenases in Biotechnology and Synthetic Biology
Abstract
Cytochromes P450 (P450 or CYP) are heme-containing enzymes that catalyze the introduction of one atom of molecular oxygen into nonactivated C-H bonds, often in a regio- and stereoselective manner. This ability, combined with a tremendous number of accepted substrates, makes P450s powerful biocatalysts. Sixty years after their discovery, P450 systems are recognized as essential bio-bricks in synthetic biology approaches to enable production of high-value complex molecules in recombinant hosts. Recent impressive results in protein engineering led to P450s with tailored properties that are even able to catalyze abiotic reactions. The introduction of P450s in artificial multi-enzymatic cascades reactions and chemo-enzymatic processes offers exciting future perspectives to access novel compounds that cannot be synthesized by nature or by chemical routes.
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Cites
Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
Ajikumar, P. K., Xiao, W.-H., Tyo, K. E. J., Wang, Y., Simeon, F., Leonard, E., Mucha, O., Phon, T. H., Pfeifer, B., & Stephanopoulos, G. (2010) · Science · doi:10.1126/science.1191652 · Cited by 1,677
Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes
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Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
Paddon, C. J., & Keasling, J. D. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3240 · Cited by 675
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Whitehouse, C. J. C., Bell, S. G., & Wong, L.-L. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15192d · Cited by 669
Pigments of rat liver microsomes
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Directed evolution of cytochrome c for carbon–silicon bond formation: Bringing silicon to life
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Cytochrome P450 monooxygenases: an update on perspectives for synthetic application
Urlacher, V. B., & Girhard, M. (2012) · Trends in Biotechnology · doi:10.1016/j.tibtech.2011.06.012 · Cited by 493
Self-citationBiocatalytic Oxidation Reactions: A Chemist's Perspective
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De novo production of the plant-derived alkaloid strictosidine in yeast
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Cited by
Hu, M., & Scott, C. (2024) · Applied and Environmental Microbiology · doi:10.1128/aem.00157-24 · Cited by 63
Metadata
- Authors
- Urlacher, V. B., & Girhard, M.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2019-08
- Volume
- 37
- Issue
- 8
- Pages
- 882-897
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2914012452
- Cited by
- 355
- References
- 83
- FWCI
- 3.00
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
