Cited by 79Protein Hydrolysis and Bioactive PeptidesProteins in Food SystemsUltrasound and Cavitation Phenomena

Impact of ultrasound pretreatment on hydrolysate and digestion products of grape seed protein

Open access
Ding, Qingzhi, Zhang, Ting, Niu, Shuai, Cao, Feifan, Wu-Chen, Ricardo Antonio, Luo, Lin, Ma, Haile·2018·Ultrasonics Sonochemistry

Abstract

The effects of ultrasound pretreatment with different working modes, including mono frequency ultrasound (MFU), simultaneous dual frequency ultrasound (SDFU) and alternate dual frequency ultrasound (ADFU) using energy-gather counter flow ultrasound equipment, on the degree of hydrolysis (DH) of grape seed protein (GSP) hydrolysate and IC 50 of GSP digestion products were studied. Amino acid composition analysis (AACA), ultraviolet–visible (UV) spectroscopy and atomic force microscopy (AFM) of GSP with different ultrasound pretreatments were measured. The results showed that MFU, SDFU and ADFU pretreatments improved the DH and reduced the IC 50 of GSP significantly (P < .05). The MFU of 20 kHz and SDFU of 20/40 kHz showed higher ACE inhibitory activity within the MFU and SDFU groups, respectively. ADFU of 20/35 kHz produced the highest ACE inhibitory activity among the three working modes (MFU, SDFU and ADFU). AACA showed that all the working modes of the ultrasound pretreatment could increase the amount of hydrophobic amino acids and the total amino acids. The changes in UV spectra and amino acid analysis indicated the unfolding of protein structure and exposure of more hydrophobic groups by SDFU and ADFU pretreatments. AFM analysis of the GSP indicated that the microstructures were destroyed and the particle size reduced after dual-frequency ultrasound pretreatments. Therefore, energy-gather counter flow ultrasound pretreatment is an effective method to improve the DH and reducing the IC 50 due to the changes of molecular conformation and effects on the microstructure by sonochemistry of GSP. In conclusion, it is necessary to select the frequency and working modes of ultrasound pretreatment for the preparation of ACE inhibitory peptide of GSP.

Integrity signals

Registry

Crossref · 10.1016/j.ultsonch.2017.11.027

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Ding, Q., Zhang, T., Niu, S., Cao, F., Wu-Chen, R. A., Luo, L., & Ma, H.
Affiliations
Jiangsu University (CN)
Corresponding author
Ma, Haile
Venue
Ultrasonics Sonochemistry
Publisher
Elsevier BV
Published
2018-04
Volume
42
Pages
704-713
ISSN
1350-4177 (ISSN-L)
OpenAlex
W2769640110
Cited by
79
References
39
Citations by year
2026: 10 · 2025: 9 · 2024: 11 · 2023: 7 · 2022: 12 · 2021: 11 · 2020: 8 · 2019: 8 · 2018: 3
FWCI
2.12
Type
journal article
Language
en
Open access
Locations
  • Ultrasonics Sonochemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Jiangsu Province (BE2016352, 1302013A, 1501105B, JAPP2012-5)
  • National Natural Science Foundation of China (31071502)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Physical Sciences › Materials Science › Materials Chemistry › Ultrasound and Cavitation Phenomena
Keywords
ultrasonic pretreatment, dual-frequency ultrasound, ACE inhibitory peptides, proteolysis, degree of hydrolysis, protein unfolding, amino acid composition, protein microstructure, IC50
MeSH terms
Ultrasonic Waves, Digestion, Hydrolysis, Pepsin A, Plant Proteins, Seeds, Vitis, Biomimetics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review