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Cited by 15Immunotherapy and Immune ResponsesImmune Response and InflammationT-cell and B-cell Immunology

Isolation and characterization of equine nasal mucosal CD172a + cells.

Baghi, Hossein Bannazadeh, Laval, Kathlyn, Favoreel, Herman, Nauwynck, Hans J.·2014·Veterinary Immunology and Immunopathology
DOI 10.1016/j.vetimm.2013.12.001
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Abstract

The nasal mucosa surface is continuously confronted with a broad variety of environmental antigens, ranging from harmless agents to potentially harmful pathogens. This area is under rigorous control of professional antigen presenting cells (APCs), such as dendritic cells (DCs) and macrophages. Mucosal APCs play a crucial role in inducing primary immune responses and the establishment of an immunological memory. In the present study, a detailed characterization of CD172a(+) cells, containing the APCs residing in the equine nasal mucosa was performed for the first time. CD172a(+) cells were isolated from collagenase-treated equine nasal mucosa fragments by MACS. Expression of surface markers was determined by flow cytometry and functional analysis was done by measuring the uptake of FITC conjugated ovalbumin (FITC-OVA). Cell surface phenotype of the isolated cells was as follows: 90% CD172a(+), 30% CD1c(+), 46% CD83(+), 42% CD206(+) and 28% MHC II(+). This clearly differs from the phenotype of blood-derived monocytes: 96% CD172a(+), 4% CD1c(+), 11% CD83(+), 9% CD206(+), 72% MHC II(+) and blood monocyte derived DCs: 99% CD172a(+), 13% CD1c(+), 30% CD83(+), 51% CD206(+) and 93% MHC II(+). The CD172a(+) nasal mucosal cells were functionally able to endocytose FITC-OVA but to a lesser degree than monocyte-derived DCs. Together, these results demonstrate that the isolated CD172a(+) nasal mucosal cells resemble immature DCs in the nasal area.

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  • The leukocytes that carry EHV-1 across the basement membrane are mainly CD172a+ cells of myeloid origin, followed by T lymphocytes and B lymphocytes.

    “These cells were mainly identified as CD172a+ cells (myeloid origin) followed by T lymphocytes and B lymphocytes (Gryspeerdt et al., 2010; Vandekerckhove et al., 2010; Baghi et al., 2014).”

    From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Baghi et al., 2014

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Authors
Baghi, H. B., Laval, K., Favoreel, H., & Nauwynck, H. J.
Affiliations
Ghent University (BE)
Corresponding author
Nauwynck, Hans J.
ORCID
Venue
Veterinary Immunology and Immunopathology
Publisher
Elsevier BV
Published
2014-02
Volume
157
Issue
3-4
Pages
155-163
ISSN
0165-2427 (ISSN-L)
DOI
10.1016/j.vetimm.2013.12.001
OpenAlex
W2028916519
Cited by
15
References
60
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 2
FWCI
0.92
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Immunology and Immunopathology (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
Funders
Research Council of Ghent University (01G01311)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
dendritic cells, antigen-presenting cells, mucosal immunity, CD1c, CD83, CD206, MHC class II, monocyte-derived dendritic cells
MeSH terms
Animals, Antigen-Presenting Cells, Antigens, Differentiation, Cell Separation, Horses, Nasal Mucosa

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