Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission
Open accessAbstract
A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.
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DC-SIGN binding to HIV-1 gp120 and HTLV-1 gp46 mediates viral particle capture, internalization, and transmission to T cells.
“DC-SIGN binding to HIV-1 gp120 and HTLV-1 gp46 has been shown to mediate viral particle capture, internalization, and transmission to T cells [74,78,79,80].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [80]
The Introduction describes DC-SIGN-mediated HIV-1 transmission to CD4+ T cells, and Fig. 1B describes HIV-1 capture and transmission by DC-SIGN-expressing cells (page numbers unavailable). Our copy does not establish binding specifically to gp120 or address HTLV-1 gp46; internalization appears only in the title of a cited work, not as a finding of this paper. (LLM, conf=0.87)
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HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
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- Authors
- Wu, L., Martin, T. D., Carrington, M., & KewalRamani, V. N.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- KewalRamani, Vineet N.
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 318
- Issue
- 1
- Pages
- 17-23
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2049631802
- Cited by
- 131
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 3 · 2013: 7 · 2012: 10
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Health and Human Services
- National Institutes of Health (n01co12400-79-0-22, NO1-CO12400)
- National Cancer Institute (CO-12400, N01CO12400, NO1‐CO‐12400)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Keywords
- DC-SIGN, HIV-1 transmission, Raji cells, THP-1 cells, dendritic cells, B cells, HIV-1 entry, cell-to-cell transmission
- MeSH terms
- DC-Specific ICAM-3 Grabbing Nonintegrin, B-Lymphocytes, Cell Line, Humans, Monocytes, Receptors, Cell Surface, HIV-1, HIV Infections, Cell Adhesion Molecules, Lectins, C-Type
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