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Cited by 67Developmental Biology and Gene RegulationRenal and related cancersThyroid Disorders and Treatments

Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning

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Fagman, Henrik, Amendola, Elena, Parrillo, Luca, Zoppoli, Pietro, Marotta, Pina, Scarfò, Marzia, De Luca, Pasquale, de Carvalho, Denise Pires +3 more·2011·Developmental Biology
DOI 10.1016/j.ydbio.2011.08.015PMC PMC3206993
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Abstract

The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.

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  • Tbx3 is a thyroid bud-specific factor as early as E10.5.

    “previous microarray combined with laser capture microdissection analysis identifies Tbx3 as a thyroid bud-specific factor at early as E10.548.”

    From Proteostatic reactivation of the developmental transcription factor TBX3 drives BRAF/MAPK-mediated tumorigenesis (2024) · cites this paper as [48]

    In “Genes enriched in the E10.5 thyroid bud” (page not provided), the paper reports Tbx3 expression in the thyroid bud at E10.5. It also reports Tbx3 in the first pharyngeal arch and mesenchyme surrounding the lung bud and trachea, so “thyroid bud-specific” overstates its distribution. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Authors
Fagman, H., Amendola, E., Parrillo, L., Zoppoli, P., Marotta, P., Scarfò, M., De Luca, P., de Carvalho, D. P., Ceccarelli, M., De Felice, M., & Di Lauro, R.
Affiliations
  • Biogem (IT)
  • Stazione Zoologica Anton Dohrn (IT)
  • University of Sannio (IT)
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2011-11
Volume
359
Issue
2
Pages
163-175
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2011.08.015
PMC
PMC3206993
OpenAlex
W2002737460
Cited by
67
References
4
Citations by year
2026: 3 · 2025: 2 · 2024: 4 · 2022: 5 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 6 · 2017: 8 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 5 · 2012: 5
FWCI
2.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • EMBO
  • Västra Götalandsregionen
  • Assar Gabrielssons Foundation
  • Telethon (GGP05161)
  • European Community (LSHM-CT-2005-01865)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
Keywords
thyroid development, lung development, organogenesis, gene expression profiling, Bcl-2, anti-apoptotic genes, cell survival, mouse embryo
MeSH terms
Animals, Digestive System, Embryo, Mammalian, Female, Lung, Male, Mice, Inbred C57BL, Thyroid Gland, Time Factors, In Situ Hybridization, Mice, Knockout, Gene Expression Regulation, Developmental, Body Patterning, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Organogenesis, Mice, Transcriptome, Laser Capture Microdissection

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