CoChat
Sign Up
Cited by 33

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

A role for Hrs in endosomal sorting of ligand-stimulated and unstimulated epidermal growth factor receptor

Morino, Chitose, Kato, Masaki, Yamamoto, Akitsugu, Mizuno, Emi, Hayakawa, Akira, Komada, Masayuki, Kitamura, Naomi·2004·Experimental Cell Research
DOI 10.1016/j.yexcr.2004.03.038
Open this paper in CoChat — free account

Abstract

Ligand-stimulated growth factor receptors are rapidly internalized and transported to early endosomes. Unstimulated receptors are also internalized constitutively, although at a slower rate, and delivered to the same organelle. At early endosomes, stimulated receptors are sorted for the lysosomal degradation pathway, whereas unstimulated receptors are mostly recycled back to the cell surface. To investigate the role of Hrs, an early endosomal protein, in this sorting process, we overexpressed Hrs in HeLa cells and examined the intracellular trafficking of epidermal growth factor receptor (EGFR) in EGF-stimulated and unstimulated cells. Overexpression of Hrs inhibited the trafficking of EGFR from early endosomes, resulting in an accumulation of EGFR on early endosomes in both ligand-stimulated and unstimulated cells. On the other hand, overexpression of Hrs mutants with a deletion or a point mutation within the FYVE domain did not inhibit the trafficking. These results suggest that Hrs regulates the sorting of ligand-stimulated and unstimulated growth factor receptors on early endosomes, and that the FYVE domain, which is required for Hrs to reside in a microdomain of early endosomes, plays an essential role in the function of Hrs.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.yexcr.2004.03.038

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • STAM-mediated recruitment of USP8 for deubiquitination at the ESCRT-0 complex may curtail EGFR sorting into late endosomal compartments.

    “As Hrs and STAM comprise the ESCRT-0 complex implicated in the early steps of receptor trafficking (18,50), our observations, therefore, imply that STAM-mediated recruitment of USP8 for deubiquitination at this juncture may curtail EGFR sorting into the late endosomal compartments.”

    From Regulation of Epidermal Growth Factor Receptor Ubiquitination and Trafficking by the USP8·STAM Complex (2010) · cites this paper as [50]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 41

  1. Schlessinger, 2000, Cell signaling by receptor tyrosine kinase, Cell, 103, 211, 10.1016/S0092-8674(00)00114-8 · DOI · Paper page
  2. Sorkin, 1998, Endocytosis and intracellular sorting of receptor tyrosine kinases, Front. Biosci., 3, 729, 10.2741/A316 · DOI
  3. Komada, 1997, Hrs, a tyrosine kinase substrate with a conserved double zinc finger domain, is localized to the cytoplasmic surface of early endosomes, J. Biol. Chem., 272, 20538, 10.1074/jbc.272.33.20538 · DOI
  4. Komada, 2001, Hrs and Hbp: possible regulators of endocytosis and exocytosis, Biochem. Biophys. Res. Commun., 281, 1065, 10.1006/bbrc.2001.4441 · DOI
  5. Komada, 1995, Growth factor-induced tyrosine phosphorylation of Hrs, a novel 115-kilodalton protein with a structurally conserved putative zinc finger domain, Mol. Cell. Biol., 15, 6213, 10.1128/MCB.15.11.6213 · DOI · Paper page
  6. Chin, 2001, Hrs interacts with sorting nexin 1 and regulates degradation of epidermal growth factor receptor, J. Biol. Chem., 276, 7069, 10.1074/jbc.M004129200 · DOI
  7. Raiborg, 2001, Hrs recruits clathrin to early endosomes, EMBO J., 20, 5008, 10.1093/emboj/20.17.5008 · DOI
  8. Bishop, 2002, Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates, J. Cell Biol., 157, 91, 10.1083/jcb.200112080 · DOI
  9. Takata, 2000, A Hrs binding protein having a Src homology 3 domain is involved in intracellular degradation of growth factors and their receptors, Genes Cells, 5, 57, 10.1046/j.1365-2443.2000.00303.x · DOI · Paper page
  10. Bean, 2000, Hrs-2 regulates receptor-mediated endocytosis via interactions with eps15, J. Biol. Chem., 275, 5271, 10.1074/jbc.275.20.15271 · DOI
  11. Kurten, 1996, Enhanced degradation of EGF receptors by a sorting nexin, SNX1, Science, 272, 1008, 10.1126/science.272.5264.1008 · DOI · Paper page
  12. Benmerah, 1998, AP-2/Eps15 interaction is required for receptor-mediated endocytosis, J. Cell Biol., 140, 1055, 10.1083/jcb.140.5.1055 · DOI
  13. Lloyd, 2002, Hrs regulates endosome membrane invagination and tyrosine kinase receptor signaling in Drosophila, Cell, 108, 261, 10.1016/S0092-8674(02)00611-6 · DOI · Paper page
  14. Bache, 2003, STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes, J. Biol. Chem., 278, 12513, 10.1074/jbc.M210843200 · DOI · Paper page
  15. Hammond, 2003, Endosomal dynamics of Met determine signaling output, Mol. Biol. Cell, 14, 1346, 10.1091/mbc.e02-09-0578 · DOI
  16. Mu, 1995, EEA1, an early endosome-associated protein, J. Biol. Chem., 270, 13503, 10.1074/jbc.270.22.13503 · DOI
  17. Yamamoto, 1995, Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast, Mol. Biol. Cell, 6, 525, 10.1091/mbc.6.5.525 · DOI
  18. Piper, 1995, VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae, J. Cell Biol., 131, 603, 10.1083/jcb.131.3.603 · DOI
  19. Weisman, 1992, Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting, J. Biol. Chem., 267, 618, 10.1016/S0021-9258(18)48539-0 · DOI
  20. Webb, 1997, Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome, Mol. Biol. Cell, 8, 871, 10.1091/mbc.8.5.871 · DOI
  21. Burd, 1998, Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains, Mol. Cell, 2, 157, 10.1016/S1097-2765(00)80125-2 · DOI
  22. Patki, 1998, A functional PtdIns(3)P-binding motif, Nature, 394, 433, 10.1038/28771 · DOI
  23. Raiborg, 2001, FYVE and coiled-coil domains determine the specific localization of Hrs to early endosomes, J. Cell. Sci., 114, 2255, 10.1242/jcs.114.12.2255 · DOI
  24. Hayakawa, 2000, Early endosomal localization of Hrs requires a sequence within the proline- and glutamine-rich region but not the FYVE finger, J. Biol. Chem., 275, 29636, 10.1074/jbc.M002696200 · DOI
  25. Komada, 1993, The cell dissociation and motility triggered by scatter factor/hepatocyte growth factor are mediated through the cytoplasmic domain of the c-Met receptor, Oncogene, 8, 2381
  26. Mizuno, 2003, STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif, Mol. Biol. Cell, 14, 3675, 10.1091/mbc.e02-12-0823 · DOI
  27. Nakamura, 2000, Syntaxin 7 mediates endocytic trafficking to late endosomes, J. Biol. Chem., 275, 6523, 10.1074/jbc.275.9.6523 · DOI
  28. Asao, 1997, Hrs is associated with STAM, a signal-transducing adaptor molecule, J. Biol. Chem., 272, 32785, 10.1074/jbc.272.52.32785 · DOI
  29. Lohi, 1998, EAST, an epidermal growth factor receptor- and Eps15-associated protein with Src homology 3 and tyrosine-based activation motif domains, J. Biol. Chem., 273, 21408, 10.1074/jbc.273.33.21408 · DOI
  30. Katz, 2002, Ligand-independent degradation of epidermal growth factor receptor involves receptor ubiquitylation and Hgs, an adaptor whose ubiquitin-interacting motif targets ubiquitylation by Nedd4, Traffic, 3, 740, 10.1034/j.1600-0854.2002.31006.x · DOI
  31. Thien, 2001, Cb1: many adaptations to regulate protein tyrosine kinases, Nat. Rev., Mol. Cell Biol., 2, 294, 10.1038/35067100 · DOI
  32. Dupre, 2001, Membrane transport: ubiquitylation in endosomal sorting, Curr. Biol., 11, R932, 10.1016/S0960-9822(01)00558-9 · DOI
  33. Raiborg, 2002, Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes, Nat. Cell Biol., 4, 394, 10.1038/ncb791 · DOI · Paper page
  34. Polo, 2002, Fiore, A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins, Nature, 416, 451, 10.1038/416451a · DOI · Paper page
  35. Katzmann, 2001, Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I, Cell, 106, 145, 10.1016/S0092-8674(01)00434-2 · DOI
  36. Babst, 2000, Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking, Traffic, 1, 248, 10.1034/j.1600-0854.2000.010307.x · DOI
  37. Li, 2002, Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking, J. Biol. Chem., 277, 28212, 10.1074/jbc.M111612200 · DOI
  38. Lohi, 2002, VHS domain—A longshoreman of vesicle lines, FEBS Lett., 513, 19, 10.1016/S0014-5793(01)03287-2 · DOI
  39. Puertollano, 2001, Sorting of mannose 6-phosphate receptors mediated by the GGAs, Science, 292, 1712, 10.1126/science.1060750 · DOI · Paper page
  40. Zhu, 2001, Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor, Science, 292, 1716, 10.1126/science.1060896 · DOI · Paper page
  41. Takatsu, 2001, Golgi-localizing, γ-adaptin ear homology domain, ADP-ribosylation factor-binding (GGA) proteins interact with acidic dileucine sequences within the cytoplasmic domains of sorting receptors through their Vps27p/Hrs/STAM (VHS) domains, J. Biol. Chem., 276, 28541, 10.1074/jbc.C100218200 · DOI

Cited by

Sorted by citations

Metadata

Authors
Morino, C., Kato, M., Yamamoto, A., Mizuno, E., Hayakawa, A., Komada, M., & Kitamura, N.
Venue
Experimental Cell Research
Publisher
Elsevier BV
Published
2004-07
Volume
297
Issue
2
Pages
380-391
ISSN
0014-4827
DOI
10.1016/j.yexcr.2004.03.038
Cited by
33
References
2
Language
en
Licence
Funders
Ministry of Education, Culture, Sports, Science and Technology (15370053)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review