Claudin expression in the bone-lining cells of female rats exposed to long-standing acidemia
Abstract
Besides enhancing osteoclast-mediated bone resorption, chronic metabolic acidosis (CMA) induces mineral efflux across the epithelial-like bone membrane formed by bone-lining cells (inactive osteoblasts), possibly via the paracellular pathway. However, there was a compensatory mechanism that restricted bone loss in the late phase of CMA, and changes in the expression of claudins, which are tight junction proteins known to regulate epithelial barrier function, were therefore anticipated in bone-lining cells. Herein, primary rat osteoblasts were found to express several transcripts of claudins, i.e., claudin-5, -11, -14, -15 and -16. Their protein expressions in bone-lining cells were demonstrated by immunohistochemistry in decalcified tibial sections. After exposure to CMA induced by oral administration of 1.5% NH(4)Cl for 21 days, expression of claudin-14, which normally seals the paracellular space and restricts ion movement, was increased, whereas that of claudin-15 and -16 which form pores for ion transport were decreased. Expressions of claudin-5 and -11 were not changed by CMA. In conclusion, the bone-lining cells of rats exposed to CMA for 21 days upregulated an ion-restrictive claudin (i.e., claudin-14), while downregulating ion-permeable claudins (i.e., claudin-15 and -16). These cellular responses might be parts of a compensatory mechanism accounting for deceleration of bone loss in late CMA.
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The BLC membrane expresses tight junction membrane proteins that are responsive to metabolic demands such as chronic metabolic acidosis.
“Indeed, the BLC membrane expresses tight junction membrane proteins that are responsive to metabolic demands such as chronic metabolic acidosis.116”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [116]
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- Authors
- Wongdee, K., Riengrojpitak, S., Krishnamra, N., & Charoenphandhu, N.
- Affiliations
- Burapha University (TH)
- Mahidol University (TH)
- Corresponding author
- Charoenphandhu, Narattaphol
- Venue
- Experimental and Molecular Pathology
- Publisher
- Elsevier BV
- Published
- 2010-04
- Volume
- 88
- Issue
- 2
- Pages
- 305-310
- ISSN
- 0014-4800 (ISSN-L)
- OpenAlex
- W1985960044
- Cited by
- 14
- References
- 37
- Citations by year
- 2022: 1 · 2019: 1 · 2018: 2 · 2016: 1 · 2014: 1 · 2013: 1 · 2012: 4
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental and Molecular Pathology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Nephrology › Renal function and acid-base balance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- claudins, bone lining cells, chronic metabolic acidosis, CLDN14, claudin-15, claudin-16, claudin-5, claudin-11, tight junctions, paracellular pathway, osteoblasts, ammonium chloride
- MeSH terms
- Acidosis, Ammonium Chloride, Animals, Female, Membrane Proteins, Nerve Tissue Proteins, Osteoblasts, Tibia, Transcription, Genetic, Gene Expression, Polymerase Chain Reaction, DNA Primers, Cell Culture Techniques, Rats, Claudins, Claudin-5
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