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Cited by 46Bone Metabolism and DiseasesBone health and osteoporosis researchBone and Dental Protein Studies

Ion microprobe analysis of mouse calvariae in vitro: evidence for a "bone membrane"

Bushinsky, D. A., Chabala, J. M., Levi-Setti, R.·1989·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.1989.256.1.e152
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Abstract

It is not clear whether the bone mineral is in passive physicochemical equilibrium with the extracellular fluid (ECF) or is separated from it by a metabolically active partition, a so-called "bone membrane." We used a sensitive high spatial resolution scanning ion microprobe utilizing secondary ion mass spectrometry to compare the relative concentrations of 23Na, 39K, and 40Ca on the surface, subsurface, and cross section of cultured live bone with the concentrations in similar regions of dead bone. Calvariae from neonatal mice were dissected and either incubated for 24 h (live) or subjected to 3 freeze-thaw cycles to kill the bone cells prior to incubation (dead). The live bone has abundant surface Na and K relative to Ca and the Na/K is approximately unity. With dead bone there is a dramatic fall in the K/Ca and an increase in the Na/K. These findings are most consistent with an egress of bone K after cell death. Flux measurements indicate a net influx of Ca into the dead bone. The marked change in relative ion concentrations with cell death indicates that live bone is not in passive physiochemical equilibrium with the surrounding medium. There appears to be a metabolically active partition, a so-called bone membrane, between the mineral and the culture medium that utilizes bone cells to maintain ion gradients.

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Crossref · 10.1152/ajpendo.1989.256.1.e152

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  • BEF has a different ionic composition from systemic extracellular fluid (SEF) and establishes an extensive contact surface (215 m2, 120-fold the size of the trabecular network) with the mineralized matrix to allow efficient, short-term mineral exchange with SEF.

    “BEF has a different ionic composition from systemic extracellular fluid (SEF)22 and establishes an extensive contact surface (215 m2, which is 120-fold the size of the trabecular network) with the mineralized matrix to allow efficient, short-term mineral exchange with SEF.23–26”

    From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [23]

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Authors
Bushinsky, D. A., Chabala, J. M., & Levi-Setti, R.
Affiliations
  • University of Chicago (US)
  • Nephrology Program, Pritzker School of Medicine, University ofChicago, Illinois 60637.
ORCID
Bushinsky, D. A.
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
1989-01-01
Volume
256
Issue
1
Pages
E152-E158
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.1989.256.1.e152
OpenAlex
W2418930860
Cited by
46
References
22
Citations by year
2024: 1 · 2022: 1 · 2018: 1 · 2014: 3 · 2012: 1
FWCI
0.38
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (P01AM033949)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 39906)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
bone membrane, bone mineral, extracellular fluid, ion gradients, sodium, potassium, calcium, freeze-thaw, calvaria, ion flux, secondary ion mass spectrometry
MeSH terms
Animals, Animals, Newborn, Bone and Bones, Calcium, Cell Membrane, Chemistry, Physical, Extracellular Space, Minerals, Organ Culture Techniques, Potassium, Sodium, Mass Spectrometry, Mice, Chemical Phenomena

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