Large dense-core vesicle exocytosis in pancreatic β-cells monitored by capacitance measurements
Abstract
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Capacitance measurements report the increase in cell surface area that occurs when the secretory granule fuses with the plasma membrane.
“Measurements of membrane capacitance report the increase in cell surface area that occurs when the secretory granule fuses with the plasma membrane (334, 505) (Figure 17A).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [334]
Cites
Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells
Olofsson, C. S., Göpel, S. O., Barg, S., Galvanovskis, J., Ma, X., Salehi, A., Rorsman, P., & Eliasson, L. (2002) · Pflügers Archiv · doi:10.1007/s00424-002-0781-5 · Cited by 290 · see citing works
Self-citationVoltage‐gated and resting membrane currents recorded from B‐cells in intact mouse pancreatic islets
Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., & Rorsman, P. (1999) · The Journal of Physiology · doi:10.1111/j.1469-7793.1999.00717.x · Cited by 226 · see citing works
Self-citationTight coupling between electrical activity and exocytosis in mouse glucagon-secreting alpha-cells.
Barg, S., Galvanovskis, J., Göpel, S. O., Rorsman, P., & Eliasson, L. (2000) · Diabetes · doi:10.2337/diabetes.49.9.1500 · Cited by 209 · see citing works
Self-citationPriming of insulin granules for exocytosis by granular Cl(-) uptake and acidification
Barg, S., Huang, P., Eliasson, L., Nelson, D. J., Obermüller, S., Rorsman, P., Thévenod, F., & Renström, E. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.11.2145 · Cited by 194 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Self-citationGöpel, S., Zhang, Q., Eliasson, L., Ma, X., Galvanovskis, J., Kanno, T., Salehi, A., & Rorsman, P. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2003.059675 · Cited by 160 · see citing works
Self-citationGoehring, I., Gerencser, A. A., Schmidt, S., Brand, M. D., Mulder, H., & Nicholls, D. G. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.314567 · Cited by 42 · see citing works
- Authors
- Kanno, T., Ma, X., Barg, S., Eliasson, L., Galvanovskis, J., Göpel, S., Larsson, M., Renström, E., & Rorsman, P.
- Affiliations
- Hirosaki University (JP)
- Lund University (SE)
- Corresponding author
- Rorsman, Patrik
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2004-08
- Volume
- 33
- Issue
- 4
- Pages
- 302-311
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W2003096362
- Cited by
- 44
- References
- 4
- Citations by year
- 2024: 3 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 1 · 2013: 1 · 2012: 4
- FWCI
- 3.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Methods (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- exocytosis, dense core vesicles, pancreatic beta cells, cell membrane capacitance, insulin secretion, electrical coupling, pancreatic islets, endocrine cells
- MeSH terms
- Animals, Cytoplasmic Granules, Exocytosis, Islets of Langerhans, Synaptic Vesicles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
