Cited by 107Mass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsClick Chemistry and Applications

XL-MS: protein cross-linking coupled with mass spectrometry

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Holding, Andrew N.·2015·Methods

Abstract

With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions.

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Crossref · 10.1016/j.ymeth.2015.06.010

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  • Cross-linking mass spectrometry has recently been very successful in generating networks of proximal amino acids in a protein or between proteins in a protein complex.

    “Moving from atoms to residues, cross-linking MS has recently been very successful in generating networks of proximal amino acids in a protein, or between proteins in a protein complex (Holding, 2015).”

    From Mapping the multiscale structure of biological systems (2021) · cites this paper as Holding, 2015

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  1. D. Suckau, M. Mak, M. Przybylski, Proc. Natl. Acad. Sci. 89 (1992) 5630-5634.
  2. G.E. Davies, G.R. Stark, Proc. Natl. Acad. Sci. 66 (1970) 651-656.
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  4. A. Leitner, L.A. Joachimiak, P. Unverdorben, T. Walzthoeni, J. Frydman, F. Frster, R. Aebersold, Proc. Natl. Acad. Sci. 111 (2014) 9455-9460.
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  6. A.N. Holding, E. Stephens, in: 59th ASMS Conference Proceedings, 2011.
  7. K.M. Pearson, L.K. Pannell, H.M. Fales, Rapid Commun. Mass Spectrom. 16 (2002) 149-159.

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Authors
Holding, A. N.
Affiliations
University of Cambridge (GB)
Corresponding author
Holding, Andrew N.
Venue
Methods
Publisher
Elsevier BV
Published
2015-11
Volume
89
Pages
54-63
ISSN
1046-2023 (ISSN-L)
OpenAlex
W653337582
Cited by
107
References
86
Citations by year
2026: 5 · 2025: 5 · 2024: 7 · 2023: 2 · 2022: 11 · 2021: 11 · 2020: 9 · 2019: 19 · 2018: 15 · 2017: 11 · 2016: 9 · 2015: 3
FWCI
1.23
Type
journal article
Language
en
Open access
Locations
  • Methods (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Apollo (University of Cambridge) (submittedVersion, cc-by)
Funders
Medical Research Council – United Kingdom
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
Keywords
protein cross-linking, cross-linking mass spectrometry, covalent cross-links, transient protein interactions, protein structure, protein organization, protein-protein interaction
MeSH terms
Animals, Cross-Linking Reagents, Humans, Proteins, Mass Spectrometry, Protein Interaction Mapping, Proteomics

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