XL-MS: protein cross-linking coupled with mass spectrometry
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With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions.
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Cross-linking mass spectrometry has recently been very successful in generating networks of proximal amino acids in a protein or between proteins in a protein complex.
“Moving from atoms to residues, cross-linking MS has recently been very successful in generating networks of proximal amino acids in a protein, or between proteins in a protein complex (Holding, 2015).”
From Mapping the multiscale structure of biological systems (2021) · cites this paper as Holding, 2015
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- Authors
- Holding, A. N.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Holding, Andrew N.
- ORCID
- Holding, Andrew N.
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2015-11
- Volume
- 89
- Pages
- 54-63
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W653337582
- Cited by
- 107
- References
- 86
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 7 · 2023: 2 · 2022: 11 · 2021: 11 · 2020: 9 · 2019: 19 · 2018: 15 · 2017: 11 · 2016: 9 · 2015: 3
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Methods (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Funders
- Medical Research Council – United Kingdom
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
- Keywords
- protein cross-linking, cross-linking mass spectrometry, covalent cross-links, transient protein interactions, protein structure, protein organization, protein-protein interaction
- MeSH terms
- Animals, Cross-Linking Reagents, Humans, Proteins, Mass Spectrometry, Protein Interaction Mapping, Proteomics
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