Bacterial colonization and the expression of inducible nitric oxide synthase in murine wounds
Open accessAbstract
The expression of inducible nitric oxide synthase (iNOS) in two different murine wound models was investigated. Animals were subjected to either full-thickness linear skin incision with subcutaneous implantation of sterile polyvinyl alcohol sponges, or to 1.5 x 1.5-cm dorsal skin excision. Reverse transcriptase-polymerase chain reaction detected iNOS mRNA in all cell samples retrieved from the sponges. Immunoblotting of lysates of inflammatory cells harvested from the sponges failed to detect iNOS protein, and immunohistochemistry of the incisional wound was mildly positive. Inflammatory cells of excisional wounds stained strongly positive for iNOS. Cutaneous wounds were found to be colonized with Staphylococcus aureus. The detection of iNOS in cells from sponges inoculated in vivo with heat-killed bacteria and the reduction of immunohistochemical signal for iNOS in excisional wounds of animals treated with antibiotics support a role of bacteria in the induction of iNOS in wounds. The expression of iNOS in excisional wounds requires interferon-gamma and functional lymphocytes because interferon-gamma knockout and SCID-Beige mice exhibited attenuated iNOS staining in excisional wounds. The expression of iNOS in the inflammatory cells of murine wounds is a response to bacterial colonization and not part of the normal repair process elicited by sterile tissue injury.
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Mp in incisional mouse skin wounds appear to express iNOS only in response to invading pathogens and not as an inherent feature of the tissue-repair response.
“However, Mp in incisional mouse skin wounds appear to express iNOS only in response to invading pathogens and not as an inherent feature of the tissue-repair response [33,67].”
From Macrophage phenotypes during tissue repair (2013) · cites this paper as [67]
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Macrophage phenotypes during tissue repair
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- Authors
- Mahoney, E., Reichner, J., Robinson Bostom, L., Mastrofrancesco, B., Henry, W., & Albina, J.
- Affiliations
- Brown University (US)
- Corresponding author
- Albina, Jorge
- ORCID
- Reichner, Jonathan
- Venue
- The American Journal of Pathology
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 161
- Issue
- 6
- Pages
- 2143-2152
- ISSN
- 0002-9440 (ISSN-L)
- PMC
- PMC1850895
- OpenAlex
- W2077985932
- Cited by
- 33
- References
- 24
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2017: 1 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal Of Pathology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM042859, GM-42859)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- inducible nitric oxide synthase, wound healing, bacterial colonization, Staphylococcus aureus, skin wounds, interferon-gamma, lymphocytes, inflammatory cells, SCID/beige mice, excision wound, incisional wounds
- MeSH terms
- Animals, Anti-Bacterial Agents, Immunohistochemistry, Interferon-gamma, Macrophages, Skin, Staphylococcus aureus, Wound Healing, Wound Infection, Surgical Sponges, Mice, SCID, Mice, Knockout, Nitric Oxide Synthase, Mice, Nitric Oxide Synthase Type II
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