Cited by 111Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Appearance of Sodium Dodecyl Sulfate-Stable Amyloid β-Protein (Aβ) Dimer in the Cortex During Aging

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Enya, Miho, Morishima-Kawashima, Maho, Yoshimura, Masahiro, Shinkai, Yasuhisa, Kusui, Kaoru, Khan, Karen, Games, Dora, Schenk, Dale +3 more·1999·The American Journal of Pathology

Abstract

We previously noted that some aged human cortical specimens containing very low or negligible levels of amyloid beta-protein (As) by enzyme immunoassay (EIA) provided prominent signals at 6 approximately 8 kd on the Western blot, probably representing sodium dodecyl sulfate (SDS)-stable Abeta dimer. Re-examination of the specificity of the EIA revealed that BAN50- and BNT77-based EIA, most commonly used for the quantitation of Abeta, capture SDS-dissociable Abeta but not SDS-stable Abeta dimer. Thus, all cortical specimens in which the levels of Abeta were below the detection limits of EIA were subjected to Western blot analysis. A fraction of such specimens contained SDS-stable dimer at 6 approximately 8 kd, but not SDS-dissociable A(beta) monomer at approximately 4 kd, as judged from the blot. This A(beta) dimer is unlikely to be generated after death, because (i) specimens with very short postmortem delay contained the A(beta) dimer, and (ii) until 12 hours postmortem, such SDS-stable A(beta) dimer is detected only faintly in PDAPP transgenic mice. The presence of A(beta) dimer in the cortex may characterize the accumulation of A(beta) in the human brain, which takes much longer than that in PDAPP transgenic mice.

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Research works printed but not linked: 2

  1. Nakabayashi J, Yoshimura M, Morishima-Kawashima M, Funato H, Miyakawa T, Yamazaki T, Ihara Y: Amyloid ␤-protein (A␤) accumula- tion in the putamen, and mamillary body during aging, and in Alzhei- mer disease. J Neuropathol Exp Neurol 1998, 57:343-352
  2. Vigo-Pelfrey C, Lee D, Keim P, Lieberburg I, Schenk DB: Character- ization of ␤-amyloid peptide from human cerebrospinal fluid. J Neu- rochem 1993, 61:1965-1968

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Authors
Enya, M., Morishima-Kawashima, M., Yoshimura, M., Shinkai, Y., Kusui, K., Khan, K., Games, D., Schenk, D., Sugihara, S., Yamaguchi, H., & Ihara, Y.
Affiliations
  • Japan Science and Technology Agency (JP)
  • Kyoto Prefectural University of Medicine (JP)
  • Tokyo Women's Medical University (JP)
  • Gunma Prefectural Cancer Center (JP)
  • Gunma University (JP)
Corresponding author
Ihara, Yasuo
Venue
The American Journal of Pathology
Publisher
Elsevier BV
Published
1999-01
Volume
154
Issue
1
Pages
271-279
ISSN
0002-9440 (ISSN-L)
OpenAlex
W2088449278
Cited by
111
References
42
Citations by year
2026: 2 · 2024: 10 · 2023: 4 · 2021: 5 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 6 · 2014: 4 · 2012: 5
FWCI
3.78
Type
journal article
Language
en
Open access
Locations
  • American Journal Of Pathology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Health, Labour and Welfare
  • Ministry of Education, Culture, Sports, Science and Technology (09835003)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
amyloid-beta, amyloid-beta dimers, cerebral cortex, brain aging, postmortem delay, amyloid accumulation, Alzheimer's disease
MeSH terms
Adult, Aged, Aged, 80 and over, Aging, Animals, Antibodies, Monoclonal, Cerebral Cortex, Drug Stability, Female, Humans, Immunoenzyme Techniques, Male, Mice, Transgenic, Middle Aged, Molecular Conformation, Sodium Dodecyl Sulfate, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Dimerization, Mice

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