Cited by 2,933Lipoproteins and Cardiovascular HealthAlzheimer's disease research and treatmentsGenetic Associations and Epidemiology

Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.

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Hixson, JE, Vernier, DT·1990·Journal of Lipid Research

Abstract

We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid positions 112 and 158. The amplification products were digested with HhaI and subjected to electrophoresis on polyacrylamide gels. Each of the isoforms was distinguished by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations. HhaI cleaves at GCGC encoding 112arg (E4) and 158arg (E3, E4), but does not cut at GTGC encoding 112cys (E2, E3) and 158cys (E2).

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Crossref · 10.1016/s0022-2275(20)43176-1

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Authors
Hixson, J., & Vernier, D.
Affiliations
Texas Biomedical Research Institute (US)
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1990-03
Volume
31
Issue
3
Pages
545-548
ISSN
0022-2275 (ISSN-L)
OpenAlex
W2111774300
Cited by
2,933
References
2
Citations by year
2026: 19 · 2025: 36 · 2024: 40 · 2023: 35 · 2022: 43 · 2021: 48 · 2020: 48 · 2019: 48 · 2018: 56 · 2017: 54 · 2016: 59 · 2015: 76 · 2014: 82 · 2013: 93 · 2012: 89
FWCI
33.40
Type
journal article
Language
en
Open access
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Heart, Lung, and Blood Institute (HL-28972, P01HL028972)
Topics
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
Keywords
apolipoprotein E, HhaI, gene amplification, apolipoprotein E isoforms
MeSH terms
Amino Acid Sequence, Apolipoproteins E, Base Sequence, DNA, Gene Amplification, Humans, Leukocytes, Molecular Sequence Data, Polymorphism, Genetic, Deoxyribonucleases, Type II Site-Specific, Polymerase Chain Reaction

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