The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
Estimating Intracellular Calcium Concentrations and Buffering without Wavelength Ratioing
Open accessAbstract
We describe a method for determining intracellular free calcium concentration ([Ca(2+)]) from single-wavelength fluorescence signals. In contrast to previous single-wavelength calibration methods, the proposed method does not require independent estimates of resting [Ca(2+)] but relies on the measurement of fluorescence close to indicator saturation during an experiment. Consequently, it is well suited to [Ca(2+)] indicators for which saturation can be achieved under physiological conditions. In addition, the method requires that the indicators have large dynamic ranges. Popular indicators such as Calcium Green-1 or Fluo-3 fulfill these conditions. As a test of the method, we measured [Ca(2+)] in CA1 pyramidal neurons in rat hippocampal slices using Oregon Green BAPTA-1 and 2-photon laser scanning microscopy (BAPTA: 1,2-bis(2-aminophenoxy)ethane-N,N,N', N'-tetraacetic acid). Resting [Ca(2+)] was 32-59 nM in the proximal apical dendrite. Monitoring action potential-evoked [Ca(2+)] transients as a function of indicator loading yielded estimates of endogenous buffering capacity (44-80) and peak [Ca(2+)] changes at zero added buffer (178-312 nM). In young animals (postnatal days 14-17) our results were comparable to previous estimates obtained by ratiometric methods (, Biophys. J. 70:1069-1081), and no significant differences were seen in older animals (P24-28). We expect our method to be widely applicable to measurements of [Ca(2+)] and [Ca(2+)]-dependent processes in small neur…
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“relative to the resting level of intracellular Ca2+ (20–50 nM) (109–111)”
From Synaptic memory and CaMKII (2023) · cites this paper as [110]
In the citing paper
“The unusually high Hill coefficient of ∼6 for CaMKII (108) and the high requirement for Ca2+ (half maximal of ∼1.6 µM at saturating CaM) compared to the resting level of Ca2+ (20–50 nM) (109, 110) ensures maintenance of the synapse specificity and Hebbian nature of LTP (19).”
From Synaptic memory and CaMKII (2023) · cites this paper as [110]
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Cites
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Zhou, S., Zhu, Q., Eom, M., Fang, S., Subach, O. M., Chen, R., Alvarado, J. S., Sunkavalli, P. S., Dong, Y., Wang, Y., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.31.635851
Resolving synaptic events using subsynaptically targeted GCaMP8 variants
Chen, J. C., Lin, J., He, K., Wang, L., Han, Y., Qiu, C., Wheeler, J. M., Daly, C. M., Macleod, G. T., & Dickman, D. (2026) · eLife · doi:10.7554/elife.107939.3
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Chen, J. C., Lin, J., He, K., Wang, L., Han, Y., Qiu, C., & Dickman, D. K. (2025) · eLife · doi:10.7554/elife.107939.1
Jenderny, S., Sacristán, D., Hövel, P., Albers, C., Beyer, I., & Ochs, K. (2025) · Neurocomputing · doi:10.1016/j.neucom.2025.131464
Regulation of Multidirectional Communication within Tripartite Synapses in the Hippocampus
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A study of the role of presenilin (1) in regulating synaptic function at hippocampal synapses
Yu, L. M. Y. (2010) · UCL Discovery (University College London)
Dendritic Integration and Reciprocal Inhibition in the Retina
Grimes, W. N. (2008) · University Libraries (University of Maryland)
Khayrullina, G., Moritz, K. E., Schooley, J. F., Fatima, N., Viollet, C., McCormack, N. M., Smyth, J. T., Doughty, M. L., Dalgard, C. L., Flagg, T. P., et al. (2020) · Figshare · doi:10.6084/m9.figshare.12276797
Khayrullina, G., Moritz, K. E., Schooley, J. F., Fatima, N., Viollet, C., McCormack, N. M., Smyth, J. T., Doughty, M. L., Dalgard, C. L., Flagg, T. P., et al. (2020) · Figshare · doi:10.6084/m9.figshare.12276782
Lai, Y., Lü, L., Sun, R., Lai, J., Wang, Y., Chen, S., Li, S., Qinglian, L., & Zhou, L. (2026) · Journal of Neuroscience · doi:10.1523/jneurosci.1202-25.2026
Resolving synaptic events using subsynaptically targeted GCaMP8 variantsPreprint
Chen, J. C., Lin, J., He, K., Wang, L., Han, Y., Qiu, C., Wheeler, J. M., Daly, C. M., Macleod, G. T., & Dickman, D. K. (2026) · eLife · doi:10.7554/elife.107939.2
Unifying experimental heterogeneity in a geometrical synaptic plasticity model
Rodrigues, Y. E. (2021) · theses.fr (ABES) · doi:10.70675/62ca5e06zd2c0z4133za51ez04d3f2ee6023
Serfilippi, T., Piccirillo, S., Preziuso, A., Terenzi, V., Ciancio, R., Magi, S., Lariccia, V., & Secondo, A. (2026) · Biomolecules · doi:10.3390/biom16050667
Metadata
- Authors
- Maravall, M., Mainen, Z. F., Sabatini, B. L., & Svoboda, K.
- Affiliations
- Cold Spring Harbor Laboratory (US)
- Corresponding author
- Svoboda, K.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2000-05
- Volume
- 78
- Issue
- 5
- Pages
- 2655-2667
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC1300854
- OpenAlex
- W1981195606
- Cited by
- 416
- References
- 46
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 18 · 2023: 13 · 2022: 11 · 2021: 10 · 2020: 13 · 2019: 19 · 2018: 25 · 2017: 18 · 2016: 17 · 2015: 23 · 2014: 22 · 2013: 21 · 2012: 31
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- intracellular calcium concentration, CA1 pyramidal neurons, hippocampal slices, dendritic calcium
- MeSH terms
- Action Potentials, Animals, Biophysics, Buffers, Calcium, Intracellular Fluid, Models, Biological, Pyramidal Cells, Calcium Signaling, Rats, Biophysical Phenomena, In Vitro Techniques
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