Amphioxus homologs of Go‐coupled rhodopsin and peropsin having 11‐cis‐ and all‐trans‐retinals as their chromophores
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Because of low contents in the native organs and failure of the expression in cultured cells, the chromophore configurations of the pigments in Go-coupled opsin and peropsin groups in the opsin family are unknown. Here we have succeeded in expression of the amphioxus homologs of these groups in HEK293s cells and found that they can be regenerated with 11-cis- and all-trans-retinals, respectively. Light isomerized the chromophores of these opsins into the all-trans and 11-cis forms, respectively. The results strongly suggest that the physiological function of peropsin would be a retinal photoisomerase, while 11-cis configuration is necessary for the Go-coupled opsin groups.
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Sp-Opsin3.1 and Sp-Opsin3.2 form a well-supported group with the Patinopecten G_o-Opsin and two Amphioxus Opsins.
“Strikingly, two Opsins, Sp-Opsin3.1 and Sp-Opsin3.2,form one well-supported group with the Patinopecten G o -Opsin (Kojima et al., 1997) and two Opsins from Amphioxus (Koyanagi et al., 2002) (Fig. 1A)”
From Opsins and clusters of sensory G-protein-coupled receptors in the sea urchin genome (2006) · cites this paper as Koyanagi et al., 2002
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- Authors
- Koyanagi, M., Terakita, A., Kubokawa, K., & Shichida, Y.
- Affiliations
- Kyoto University (JP)
- The University of Tokyo (JP)
- Corresponding author
- Shichida, Yoshinori
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2002-10-29
- Volume
- 531
- Issue
- 3
- Pages
- 525-528
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2071317669
- Cited by
- 114
- References
- 34
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 8 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 7 · 2013: 5 · 2012: 4
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Keywords
- amphioxus, rhodopsin, G protein-coupled receptors, 11-cis-retinal, all-trans-retinal, chromophore isomerization
- MeSH terms
- Animals, Cell Line, Chromatography, High Pressure Liquid, Eye Proteins, Humans, Phylogeny, Retinaldehyde, Rhodopsin, Tretinoin, Blotting, Western, GTP-Binding Proteins
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