The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Involvement of NF‐κB in the regulation of cyclooxygenase‐2 protein expression in LPS‐stimulated J774 macrophages
Abstract
We investigated the involvement of NF‐κB in the regulation of COX‐2 protein expression and prostaglandin production in LPS‐stimulated J774 macrophages. Incubation of J774 cells with LPS (1 μg/ml) for 24 h caused an increase of COX‐2 protein expression and accumulation of both PGE 2 and 6‐keto‐PGF 1α in the cell culture medium. Ammonium pyrrolidinedithiocarbamate (APDC, 0.1, 1, 10 μM) and N‐α‐p‐tosyl‐l‐lysine chloromethylketone (TLCK, 1, 10, 100 μM), two inhibitors of NF‐κB activation, suppressed in a concentration‐dependent manner both LPS‐induced COX‐2 protein expression and prostanoid generation. Moreover, APDC and TLCK both inhibited the LPS‐induced increase of NF‐κB DNA binding activity and prevented IκB‐α degradation. Our results show for the first time that NF‐κB is involved in COX‐2 protein expression in LPS‐stimulated J774 macrophages and suggest that inhibitors of NF‐κB activation may represent a useful tool for the pharmacological control of inflammation.
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Cites
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Cited by
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Perera, P.-Y., Mayadas, T. N., Takeuchi, O., Akira, S., Zaks-Zilberman, M., Goyert, S. M., & Vogel, S. N. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.1.574 · Cited by 391
NF-κB Regulates Phagocytic NADPH Oxidase by Inducing the Expression of gp91
Anrather, J., Racchumi, G., & Iadecola, C. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m506172200 · Cited by 386
Anti-inflammatory activity of macrolide antibiotics
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Metadata
- Authors
- D'Acquisto, F., Iuvone, T., Rombolà, L., Sautebin, L., Di Rosa, M., & Carnuccio, R.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-11-24
- Volume
- 418
- Issue
- 1-2
- Pages
- 175-178
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1989351016
- Cited by
- 270
- References
- 17
- FWCI
- 4.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Involvement of NF‐κB in the regulation of cyclooxygenase‐2 protein expression in LPS‐stimulated J774 macrophages. https://app.cochat.ai/papers/10.1016/s0014-5793(97)01377-x
- The paper
- D'Acquisto, F., Iuvone, T., Rombolà, L., Sautebin, L., Di Rosa, M., & Carnuccio, R. (1997). Involvement of NF‐κB in the regulation of cyclooxygenase‐2 protein expression in LPS‐stimulated J774 macrophages. FEBS Letters. https://doi.org/10.1016/s0014-5793(97)01377-x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
