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Cited by 835

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

AMP‐activated protein kinase phosphorylation of endothelial NO synthase

Open access
Chen, Zhi-Ping, Mitchelhill, Ken I, Michell, Belinda J, Stapleton, David, Rodriguez-Crespo, Ignacio, Witters, Lee A, Power, David A, Ortiz de Montellano, Paul R, Kemp, Bruce E·1999·FEBS Letters
DOI 10.1016/s0014-5793(98)01705-0
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Abstract

The AMP‐activated protein kinase (AMPK) in rat skeletal and cardiac muscle is activated by vigorous exercise and ischaemic stress. Under these conditions AMPK phosphorylates and inhibits acetyl‐coenzyme A carboxylase causing increased oxidation of fatty acids. Here we show that AMPK co‐immunoprecipitates with cardiac endothelial NO synthase (eNOS) and phosphorylates Ser‐1177 in the presence of Ca 2+‐calmodulin (CaM) to activate eNOS both in vitro and during ischaemia in rat hearts. In the absence of Ca 2+‐calmodulin, AMPK also phosphorylates eNOS at Thr‐495 in the CaM‐binding sequence, resulting in inhibition of eNOS activity but Thr‐495 phosphorylation is unchanged during ischaemia. Phosphorylation of eNOS by the AMPK in endothelial cells and myocytes provides a further regulatory link between metabolic stress and cardiovascular function.

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Crossref · 10.1016/s0014-5793(98)01705-0

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Metadata

Authors
Chen, Z.-P., Mitchelhill, K. I., Michell, B. J., Stapleton, D., Rodriguez-Crespo, I., Witters, L. A., Power, D. A., Ortiz de Montellano, P. R., & Kemp, B. E.
Venue
FEBS Letters
Publisher
Wiley
Published
1999-01-29
Volume
443
Issue
3
Pages
285-289
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
DOI
10.1016/s0014-5793(98)01705-0
OpenAlex
W2068820862
Cited by
835
References
23
FWCI
14.88
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: AMP‐activated protein kinase phosphorylation of endothelial NO synthase. https://app.cochat.ai/papers/10.1016/s0014-5793(98)01705-0
The paper
Chen, Z.-P., Mitchelhill, K. I., Michell, B. J., Stapleton, D., Rodriguez-Crespo, I., Witters, L. A., Power, D. A., Ortiz de Montellano, P. R., & Kemp, B. E. (1999). AMP‐activated protein kinase phosphorylation of endothelial NO synthase. FEBS Letters. https://doi.org/10.1016/s0014-5793(98)01705-0

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.