The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2
Open accessAbstract
Two sodium-dependent vitamin C transporters, hSVCT1 and hSVCT2, were cloned from a human kidney cDNA library. hSVCT1 had a 1797 bp open reading frame encoding a 598 amino acid polypeptide. The 1953 bp open reading frame of hSVCT2 encoded a 650 amino acid polypeptide. Using a Xenopus laevis oocyte expression system, both transporters were functionally expressed. By Eadie-Hofstee transformation the apparent K(m) of hSVCT1 for ascorbate was 252.0 microM and of hSVCT2 for ascorbate was 21.3 microM. Both transporters were sodium-dependent and did not transport dehydroascorbic acid. Incubation of oocytes expressing either transporter with phorbol 12-myristate 13-acetate (PMA) inhibited ascorbate transport activity. Availability of the human transporter clones may facilitate new strategies for determining vitamin C intake.
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“The Michaelis constant Km, i.e., the concentration of vitamin C transported by half of the maximal transport velocity, was reported to be in a wide range from 10 to 200 µM, suggesting a large capacity to absorb dietary vitamin C.”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [51]
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“Vitamin C is efficiently and saturably reabsorbed from the urinary tract, and SVCT1 plays an important role, as documented by knockout Slc23a1−/− mice with an 18-fold higher ascorbate excretion [51,80,81].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [51]
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“Vitamin C is taken up by the cells via SVCT2 (SLC23A2, Figure 2C), which is a close analogue of SVCT1 with which it shares 65% sequence homology.”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [51]
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“Its activity is highest at a neutral pH. It drops markedly with increasing acidity [51,52,53,54].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [51]
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Cites
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Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic Acid
Rumsey, S. C., Kwon, O., Xu, G. W., Burant, C., Simpson, I. A., & Levine, M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.30.18982 · Cited by 481
Self-citationMicronutrients prevent cancer and delay aging
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Absorption, transport, and disposition of ascorbic acid in humans
Rumsey, S. C., & Levine, M. (1998) · The Journal of Nutritional Biochemistry · doi:10.1016/s0955-2863(98)00002-3 · Cited by 210
Self-citationReduction of the Ascorbyl Free Radical to Ascorbate by Thioredoxin Reductase
May, J. M., Cobb, C. E., Mendiratta, S. S., Hill, K. E., & Burk, R. F. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.36.23039 · Cited by 188
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Self-citationPharmacokinetic model of ascorbic acid in healthy male volunteers during depletion and repletion
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Self-citationWells, W. W., & Xu, D. P. (1994) · Journal of Bioenergetics and Biomembranes · doi:10.1007/bf00762777 · Cited by 128
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Self-citation[14]Xenopus oocyte microinjection: From gene to protein
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Research works printed but not linked: 1
- Levine M. Rumsey S.C. Wang Y. Park J.B. Xu W. and Amano N. (1996) in: Present Knowledge in Nutrition (Filer L.J. and Ziegler E.E. Eds.) pp. 146–159 International Life Sciences Institute Washington DC.
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Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention
Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.-H., Chen, S., Corpe, C., Dutta, A., Dutta, S. K., et al. (2003) · Journal of the American College of Nutrition · doi:10.1080/07315724.2003.10719272 · Cited by 1,949
Self-citationVitamin C: The known and the unknown and Goldilocks
Padayatty, S., & Levine, M. (2016) · Oral Diseases · doi:10.1111/odi.12446 · Cited by 753
Self-citationVitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination
Doseděl, M., Jirkovský, E., Macáková, K., Krčmová, L., Javorská, L., Pourová, J., Mercolini, L., Remião, F., Nováková, L., Mladěnka, P., et al. (2021) · Nutrients · doi:10.3390/nu13020615 · Cited by 553
A new recommended dietary allowance of vitamin C for healthy young women
Levine, M., Wang, Y., Padayatty, S. J., & Morrow, J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171318198 · Cited by 496
Self-citationREGULATION OF VITAMIN C TRANSPORT
Wilson, J. X. (2005) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.25.050304.092647 · Cited by 376
Metadata
- Authors
- Daruwala, R., Song, J., Koh, W. S., Rumsey, S. C., & Levine, M.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Levine, Mark
- ORCID
- Levine, Mark
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1999-11
- Volume
- 460
- Issue
- 3
- Pages
- 480-484
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2079405203
- Cited by
- 272
- References
- 19
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 10 · 2022: 1 · 2021: 15 · 2020: 10 · 2019: 3 · 2018: 11 · 2017: 6 · 2016: 8 · 2015: 9 · 2014: 6 · 2013: 17 · 2012: 9
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- sodium-dependent vitamin C transporters, ascorbate transport, vitamin C, dehydroascorbic acid, phorbol myristate acetate, transporter inhibition, kidney, oocyte expression, Michaelis constant, vitamin C intake
- MeSH terms
- Amino Acid Sequence, Animals, Ascorbic Acid, Base Sequence, Biological Transport, Carrier Proteins, Cloning, Molecular, Bucladesine, Female, Humans, Molecular Sequence Data, Oocytes, Proteins, Sodium, Tetradecanoylphorbol Acetate, Protein Biosynthesis, Xenopus laevis, Symporters, Organic Anion Transporters, Sodium-Dependent, Sodium-Coupled Vitamin C Transporters
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