Human Placental Sodium-Dependent Vitamin C Transporter (SVCT2): Molecular Cloning and Transport Function
Abstract
We report here on the cloning and functional characterization of human SVCT2, a sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from a human placental choriocarcinoma cell cDNA library, codes for a protein of 650 amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When functionally expressed in mammalian cells, hSVCT2 induces the transport of ascorbic acid. The transport process induced by hSVCT2 is Na(+)-dependent and is specific for ascorbate. The Michaelis-Menton constant (K(t)) for the transport of ascorbate in cDNA-transfected cells is 69 +/- 5 microM. The relationship between the cDNA-specific uptake rate of ascorbate and Na(+) concentration is sigmoidal with a Na(+):ascorbate stoichiometry of 2:1. Northern blot analysis shows that SVCT2-specific transcripts are present in heart, brain, placenta, and liver and is absent in lung and skeletal muscle. The size of the principal transcript is approximately 7.5 kb.
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Replacement of sodium with other positively charged ions completely abolishes transport.
“Replacement of sodium with other positively charged ions completely abolishes transport [42].”
From The Roles of Vitamin C in Skin Health. (2017) · cites this paper as [42]
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- Authors
- Rajan, D. P., Huang, W., Dutta, B., Devoe, L. D., Leibach, F. H., Ganapathy, V., & Prasad, P. D.
- Affiliations
- Augusta University (US)
- Corresponding author
- Prasad, Puttur D.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1999-09
- Volume
- 262
- Issue
- 3
- Pages
- 762-768
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2005413052
- Cited by
- 162
- References
- 15
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 6 · 2015: 1 · 2014: 5 · 2013: 11 · 2012: 9
- FWCI
- 6.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 33347, R01HD033347)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- SVCT2, sodium-dependent vitamin C transporters, ascorbate transport, vitamin C, placenta, gene cloning, sodium dependence, Michaelis-Menten kinetics, tissue distribution, cDNA expression, choriocarcinoma cells
- MeSH terms
- Amino Acid Sequence, Animals, Ascorbic Acid, Base Sequence, Biological Transport, Cell Line, Choriocarcinoma, Cloning, Molecular, Female, Humans, Kinetics, Molecular Sequence Data, Pregnancy, Proteins, Recombinant Proteins, Sodium, Substrate Specificity, Transfection, Tumor Cells, Cultured, Uterine Neoplasms, Gene Library, Symporters, Organic Anion Transporters, Sodium-Dependent, Rats, Sodium-Coupled Vitamin C Transporters
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