A new recommended dietary allowance of vitamin C for healthy young women

Levine, Mark; Wang, Yaohui; Padayatty, Sebastian J.; Morrow, Jason

doi:10.1073/pnas.171318198

Cited by 496Vitamin C and Antioxidants ResearchAntioxidant Activity and Oxidative StressPhytochemicals and Antioxidant Activities

A new recommended dietary allowance of vitamin C for healthy young women

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Levine, Mark, Wang, Yaohui, Padayatty, Sebastian J., Morrow, Jason·2001·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.171318198PMC PMC55540

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Abstract

The recently released Recommended Dietary Allowance of vitamin C for women, 75 mg daily, was based on data for men. We now report results of a depletion-repletion study with healthy young women hospitalized for 186 +/- 28 days, using vitamin C doses of 30-2,500 mg daily. The relationship between dose and steady-state plasma concentration was sigmoidal. Only doses above 100 mg were beyond the linear portion of the curve. Plasma and circulating cells saturated at 400 mg daily, with urinary elimination of higher doses. Biomarkers of endogenous oxidant stress, plasma and urine F(2)-isoprostanes, and urine levels of a major metabolite of F(2)-isoprostanes were unchanged by vitamin C at all doses, suggesting this vitamin does not alter endogenous lipid peroxidation in healthy young women. By using Food and Nutrition Board guidelines, the data indicate that the Recommended Dietary Allowance for young women should be increased to 90 mg daily.

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Multiple studies showed no effect of vitamin C on markers of lipid peroxidation in healthy humans or animals.
“Multiple studies showed no effect of vitamin C on markers of lipid peroxidation in healthy humans or animals [63,66,166].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [66]
Plasma vitamin C levels increase to a plateau concentration up to doses of about 200-400 mg daily; higher doses increase plasma levels only minimally.
“The plasma levels increase to this plateau concentration up to doses of about 200–400 mg daily. Higher doses increase plasma levels only minimally [55,65,66].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [66]
In women, the saturable doses were 200-400 mg daily, matching doses producing maximal steady-state plasma levels.
“This was not confirmed in women where the saturable doses were 200–400 mg daily, matching with doses producing maximal steady-state plasma levels [66].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [66]

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HEALTH
Ordman, A. R. (2009)

Authors: Levine, M., Wang, Y., Padayatty, S. J., & Morrow, J.
Affiliations: National Institutes of Health (US)
ORCID: Levine, Mark
Wang, Yaohui
Padayatty, Sebastian J.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2001-08-14
Volume: 98
Issue: 17
Pages: 9842-9846
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.171318198
PMC: PMC55540
OpenAlex: W2070532527
Cited by: 496
References: 27
Citations by year: 2026: 5 · 2025: 16 · 2024: 12 · 2023: 21 · 2022: 30 · 2021: 34 · 2020: 38 · 2019: 17 · 2018: 17 · 2017: 17 · 2016: 11 · 2015: 17 · 2014: 19 · 2013: 27 · 2012: 25
FWCI: 19.48
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (DK 48831, DK 26657, GM 15431, CA 77839)
National Cancer Institute (CA77839, P01CA077839)
National Institute of General Medical Sciences (GM 15431, P50GM015431, P01GM015431)
National Institute of Diabetes and Digestive and Kidney Diseases (Z01DK054506, DK-26657, DK 48831, P30DK026657, R01DK048831)
NIH Clinical Center
Topics: Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Keywords: vitamin C, RDA, young women, dose-response relationship, steady-state plasma concentration, urinary excretion, F2-isoprostanes, lipid peroxidation, oxidative stress
MeSH terms: Adult, Ascorbic Acid, Biological Availability, Canada, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Female, Humans, Nutritional Requirements, Sex Characteristics, United States, Lipid Peroxidation, Dinoprost, Oxidative Stress, Nutrition Policy, F2-Isoprostanes

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A new recommended dietary allowance of vitamin C for healthy young women

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