The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Structure of acetylcholinesterase complexed with (−)‐galanthamine at 2.3 Å resolution
Open accessAbstract
(−)‐Galanthamine (GAL), an alkaloid from the flower, the common snowdrop (Galanthus nivalis), shows anticholinesterase activity. This property has made GAL the target of research as to its effectiveness in the treatment of Alzheimer's disease. We have solved the X‐ray crystal structure of GAL bound in the active site of Torpedo californica acetylcholinesterase (Tc AChE) to 2.3 Å resolution. The inhibitor binds at the base of the active site gorge of Tc AChE, interacting with both the choline‐binding site (Trp‐84) and the acyl‐binding pocket (Phe‐288, Phe‐290). The tertiary amine group of GAL does not interact closely with Trp‐84; rather, the double bond of its cyclohexene ring stacks against the indole ring. The tertiary amine appears to make a non‐conventional hydrogen bond, via its N‐methyl group, to Asp‐72, near the top of the gorge. The hydroxyl group of the inhibitor makes a strong hydrogen bond (2.7 Å) with Glu‐199. The relatively tight binding of GAL to Tc AChE appears to arise from a number of moderate to weak interactions with the protein, coupled to a low entropy cost for binding due to the rigid nature of the inhibitor.
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Metadata
- Authors
- Greenblatt, H. M., Kryger, G., Lewis, T., Silman, I., & Sussman, J. L.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1999-12-17
- Volume
- 463
- Issue
- 3
- Pages
- 321-326
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2000352547
- Cited by
- 310
- References
- 42
- FWCI
- 4.85
- Type
- journal article
- Language
- en
- Open access
- bronze
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