The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay.
Open accessAbstract
A simple radioisotopic assay for malonyl-CoA is described. The method is based on the malonyl-CoA-dependent incorporation of labeled acetyl-CoA into palmitic acid catalyzed by fatty acid synthetase in the presence of NADPH. Its main advantages over the more conventional spectrophotometric procedure is that it is extremely sensitive and allows the simultaneous determination of picomole quantities of malonyl-CoA in multiple tissue extracts. It should prove particularly suitable for studies on the regulation of lipid metabolism in isolated hepatocytes where the quantity of tissue available for analysis is frequently very small. Application of the method to the measurement of malonyl-CoA in livers from fed, fasted, and diabetic rats yielded values that were consistent with the recently postulated role of malonyl-CoA in the regulation of hepatic ketone body production.
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Citations
Cites
A possible role for malonyl-CoA in the regulation of hepatic fatty acid oxidation and ketogenesis.
McGarry, J. D., Mannaerts, G. P., & Foster, D. W. (1977) · Journal of Clinical Investigation · doi:10.1172/jci108764 · Cited by 736
Self-citation
Cited by
Winder, W. W., & Hardie, D. G. (1996) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1996.270.2.e299 · Cited by 799
McGarry, J. D., Mills, S. E., Long, C. S., & Foster, D. W. (1983) · Biochemical Journal · doi:10.1042/bj2140021 · Cited by 556
Self-citationHolmes, B. F., Kurth-Kraczek, E. J., & Winder, W. W. (1999) · Journal of Applied Physiology · doi:10.1152/jappl.1999.87.5.1990 · Cited by 489
An, J., Muoio, D. M., Shiota, M., Fujimoto, Y., Cline, G. W., Shulman, G. I., Koves, T. R., Stevens, R., Millington, D., & Newgard, C. B. (2004) · Nature Medicine · doi:10.1038/nm995 · Cited by 450
Contraction-induced Changes in Acetyl-CoA Carboxylase and 5′-AMP-activated Kinase in Skeletal Muscle
Vavvas, D., Apazidis, A., Saha, A. K., Gamble, J., Patel, A., Kemp, B. E., Witters, L. A., & Ruderman, N. B. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.20.13255 · Cited by 395
Metadata
- Authors
- McGarry, J. D., Stark, M. J., & Foster, D. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1978-11
- Volume
- 253
- Issue
- 22
- Pages
- 8291-8293
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1503255196
- Cited by
- 187
- References
- 1
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay. https://app.cochat.ai/papers/10.1016/s0021-9258(17)34394-6
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- McGarry, J. D., Stark, M. J., & Foster, D. W. (1978). Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)34394-6
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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