Inactivation of acetyl-CoA carboxylase and activation of AMP-activated protein kinase in muscle during exercise
Abstract
Malonyl-CoA, an inhibitor of fatty acid oxidation in skeletal muscle mitochondria, decreases in rat skeletal muscle during exercise or in response to electrical stimulation. Regulation of rat skeletal muscle acetyl-CoA carboxylase (ACC), the enzyme that synthesizes malonyl-CoA, was studied in vitro and in vivo. Avidin-Sepharose affinity-purified ACC from hindlimb skeletal muscle was phosphorylated by purified liver AMP-activated protein kinase with a concurrent decrease in ACC activity. AMP-activated protein kinase was quantitated in resuspended ammonium sulfate precipitates of the fast-twitch red (type IIa fibers) region of the quadriceps muscle. Rats running on a treadmill at 21 m/min up a 15% grade show a 2.4-fold activation of AMP-activated protein kinase concurrently with a marked decrease in ACC activity in the resuspended ammonium sulfate precipitates at all citrate concentrations ranging from 0 to 20 mM. Malonyl-CoA decreased from a resting value of 1.85 +/- 0.29 to 0.50 +/- 0.09 nmol/g in red quadriceps muscle after 30 min of treadmill running. The activation of the AMP-activated protein kinase with consequent phosphorylation and inactivation of ACC may be one of the primary events in the control of malonyl-CoA and hence fatty acid oxidation during exercise.
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Active AMPK is involved in the increase in fat oxidation during exercise.
“Active AMPK is involved in the increase in fat oxidation during exercise [33]”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [33]
Cites
(1975) · Journal of AOAC INTERNATIONAL · doi:10.1093/jaoac/58.5.1085 · Cited by 20,078 · see citing works
Corton, J. M., Gillespie, J. G., Hawley, S. A., & Hardie, D. G. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.tb20498.x · Cited by 1,232 · see citing works
Self-citationColorimetric ultramicro method for the determination of free fatty acids
Novák, M. (1965) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39315-9 · Cited by 639 · see citing works
McGarry, J. D., Mills, S. E., Long, C. S., & Foster, D. W. (1983) · Biochemical Journal · doi:10.1042/bj2140021 · Cited by 556 · see citing works
DAVIES, S. P., Carling, D., & Hardie, D. G. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb15185.x · Cited by 431 · see citing works
Self-citationPurification and characterization of the AMP‐activated protein kinase
Carling, D., Clarke, P. R., Zammit, V. A., & Hardie, D. G. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb15186.x · Cited by 418 · see citing works
Self-citationAcetyl-CoA carboxylase regulation of fatty acid oxidation in the heart.
Saddik, M., Gamble, J., Witters, L. A., & Lopaschuk, G. D. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)74465-2 · Cited by 390 · see citing works
Identification of an isozymic form of acetyl-CoA carboxylase.
Bianchi, A., Evans, J. L., Iverson, A. J., Nordlund, A. C., Watts, T. D., & Witters, L. A. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40045-8 · Cited by 261 · see citing works
Role of reversible phosphorylation of acetyl‐CoA carboxylase in long‐chain fatty acid synthesis 1
Kim, K., López‐Casillas, F., Bai, D.-H., Luo, X., & Pape, M. E. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.11.2570725 · Cited by 201 · see citing works
Malonyl-CoA metabolism in cardiac myocytes and its relevance to the control of fatty acid oxidation
Awan, M. M., & SAGGERSON, E. D. (1993) · Biochemical Journal · doi:10.1042/bj2950061 · Cited by 196 · see citing works
Regulation of fatty acid synthesis via phosphorylation of acetyl-CoA carboxylase
Hardie, D. G. (1989) · Progress in Lipid Research · doi:10.1016/0163-7827(89)90010-6 · Cited by 191 · see citing works
Self-citationMcGarry, J., Stark, M. J., & Foster, D. W. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34394-6 · Cited by 188 · see citing works
DAVIES, S. P., Carling, D., Munday, M. R., & Hardie, D. G. (1992) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1992.tb16591.x · Cited by 179 · see citing works
Self-citationDAVIES, S. P., Hawley, S. A., Woods, A., Carling, D., Haystead, T., & Hardie, D. G. (1994) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1994.tb19001.x · Cited by 173 · see citing works
Self-citationSim, A. T. R., & Hardie, D. G. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80445-9 · Cited by 148 · see citing works
Self-citationWeis, B. C., Esser, V., Foster, D. W., & McGarry, J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32222-6 · Cited by 124 · see citing works
Purification and Characterization of Rat Skeletal Muscle Acetyl-CoA Carboxylase
Trumble, G. E., Smith, M. A., & Winder, W. W. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.0192f.x · Cited by 76 · see citing works
Self-citationEffect of fasting and refeeding on acetyl-CoA carboxylase in rat hindlimb muscle
Winder, W. W., MacLean, P. S., Lucas, J. C., Fernley, J. E., & Trumble, G. E. (1995) · Journal of Applied Physiology · doi:10.1152/jappl.1995.78.2.578 · Cited by 51 · see citing works
Self-citationIdentification of human acetyl-CoA carboxylase isozymes in tissue and in breast cancer cells
Witters, L. A., Widder, J., King, A., Fassihi, K., & Kuhajda, F. P. (1994) · International Journal of Biochemistry · doi:10.1016/0020-711x(94)90018-3 · Cited by 51 · see citing works
Regulation of mitochondrial carnitine palmitoyl transferases from liver and extrahepatic tissues
Saggerson, D., Ghadiminejad, I., & Awan, M. (1992) · Advances in Enzyme Regulation · doi:10.1016/0065-2571(92)90023-s · Cited by 51 · see citing works
Verhoeven, A. J. M., Woods, A., Brennan, C. H., et al. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.0236n.x · Cited by 50 · see citing works
Lipid metabolism during exercise: Influence of training
Terjung, R. L., & Kaciuba-Uściłko, H. (1986) · Diabetes/Metabolism Reviews · doi:10.1002/dmr.5610020103 · Cited by 33 · see citing works
Nerve stimulation decreases malonyl-CoA in skeletal muscle
Duan, C., & Winder, W. W. (1992) · Journal of Applied Physiology · doi:10.1152/jappl.1992.72.3.901 · Cited by 33 · see citing works
Self-citationEvidence of a biotin dependent acetyl-coenzyme a carboxylase in rat muscle
Trumble, G. E., Smith, M. A., & Winder, W. W. (1991) · Life Sciences · doi:10.1016/0024-3205(91)90577-x · Cited by 22 · see citing works
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Sorted by citations
AMPK: a nutrient and energy sensor that maintains energy homeostasis
Hardie, D. G., Ross, F. A., & Hawley, S. A. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3311 · Cited by 4,602 · see citing works
Self-citationFiber Types in Mammalian Skeletal Muscles
Schiaffino, S., & Reggiani, C. (2011) · Physiological Reviews · doi:10.1152/physrev.00031.2010 · Cited by 3,022 · see citing works
Kahn, B. B., Alquier, T., Carling, D., & Hardie, D. G. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2004.12.003 · Cited by 2,799 · see citing works
Self-citationLack of Exercise Is a Major Cause of Chronic Diseases
Booth, F. W., Roberts, C. K., & Laye, M. J. (2012) · Comprehensive Physiology · doi:10.1002/cphy.c110025 · Cited by 2,575 · see citing works
Exercise Metabolism and the Molecular Regulation of Skeletal Muscle Adaptation
Egan, B., & Zierath, J. R. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2012.12.012 · Cited by 2,084 · see citing works
The Mitochondrial Carnitine Palmitoyltransferase System — From Concept to Molecular Analysis
McGarry, J. D., & Brown, N. F. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00001.x · Cited by 1,616 · see citing works
Hawley, S. A., Pan, D. A., Mustard, K. J., Ross, L., Bain, J., Edelman, A. M., Frenguelli, B. G., & Hardie, D. G. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.05.009 · Cited by 1,589 · see citing works
Self-citationMcGarry, J. D. (2002) · Diabetes · doi:10.2337/diabetes.51.1.7 · Cited by 1,435 · see citing works
Exercise, GLUT4, and skeletal muscle glucose uptake
Richter, E. A., & Hargreaves, M. (2013) · Physiological Reviews · doi:10.1152/physrev.00038.2012 · Cited by 1,407 · see citing works
The AMP‐Activated Protein Kinase: Fuel Gauge of the Mammalian Cell?
Hardie, D. G., & Carling, D. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00259.x · Cited by 1,317 · see citing works
Self-citationIntegrative Biology of Exercise
Hawley, J. A., Hargreaves, M., Joyner, M. J., & Zierath, J. R. (2014) · Cell · doi:10.1016/j.cell.2014.10.029 · Cited by 1,152 · see citing works
Zong, H., Ren, J. M., Young, L. H., Pypaert, M., Mu, J., Birnbaum, M. J., & Shulman, G. I. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.252625599 · Cited by 1,036 · see citing works
Fuel selection in human skeletal muscle in insulin resistance: a reexamination.
Kelley, D. E., & Mandarino, L. J. (2000) · Diabetes · doi:10.2337/diabetes.49.5.677 · Cited by 1,017 · see citing works
Minireview: The AMP-Activated Protein Kinase Cascade: The Key Sensor of Cellular Energy Status
Hardie, D. G. (2003) · Endocrinology · doi:10.1210/en.2003-0982 · Cited by 994 · see citing works
Self-citationMu, J., Brozinick, J. T., Valladares, O., Bucan, M., & Birnbaum, M. J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00251-9 · Cited by 924 · see citing works
The effects of increasing exercise intensity on muscle fuel utilisation in humans
van Loon, L. J. C., Greenhaff, P. L., Constantin‐Teodosiu, D., Saris, W. H. M., & Wagenmakers, A. J. M. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.00295.x · Cited by 897 · see citing works
AMPK and TOR: The Yin and Yang of Cellular Nutrient Sensing and Growth Control
González, A., Hall, M. N., Lin, S.-C., & Hardie, D. G. (2020) · Cell Metabolism · doi:10.1016/j.cmet.2020.01.015 · Cited by 797 · see citing works
Self-citationUse of Cells Expressing γ Subunit Variants to Identify Diverse Mechanisms of AMPK Activation
Hawley, S. A., Ross, F. A., Chevtzoff, C., Green, K. A., Evans, A., Fogarty, S., Towler, M. C., Brown, L. J., Ogunbayo, O. A., Evans, A. M., et al. (2010) · Cell Metabolism · doi:10.1016/j.cmet.2010.04.001 · Cited by 773 · see citing works
Self-citationScott, J. W., Hawley, S. A., Green, K. A., Anis, M., Stewart, G., Scullion, G. A., Norman, D. G., & Hardie, D. G. (2004) · Journal of Clinical Investigation · doi:10.1172/jci19874 · Cited by 754 · see citing works
Self-citationRussell, R. R., Li, J., Coven, D. L., Pypaert, M., Zechner, C., Palmeri, M., Giordano, F. J., Mu, J., Birnbaum, M. J., & Young, L. H. (2004) · Journal of Clinical Investigation · doi:10.1172/jci19297 · Cited by 738 · see citing works
Activation of AMP-activated protein kinase increases mitochondrial enzymes in skeletal muscle
Winder, W. W., Holmes, B. F., Rubink, D. S., Jensen, E. B., Chen, M., & Holloszy, J. O. (2000) · Journal of Applied Physiology · doi:10.1152/jappl.2000.88.6.2219 · Cited by 709 · see citing works
Self-citationHawley, S. A., Gadalla, A. E., Olsen, G. S., & Hardie, D. G. (2002) · Diabetes · doi:10.2337/diabetes.51.8.2420 · Cited by 696 · see citing works
Self-citationCharacterization of AMP-activated protein kinase γ-subunit isoforms and their role in AMP binding
CHEUNG, P. C. F., SALT, I. P., DAVIES, S. P., HARDIE, D. G., & CARLING, D. (2000) · Biochemical Journal · doi:10.1042/bj3460659 · Cited by 637 · see citing works
Self-citationDreyer, H. C., Fujita, S., Cadenas, J. G., Chinkes, D. L., Volpi, E., & Rasmussen, B. B. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.113175 · Cited by 570 · see citing works
Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis
Bergeron, R., Ren, J. M., Cadman, K. S., Moore, I. K., Perret, P., Pypaert, M., Young, L. H., Semenkovich, C. F., & Shulman, G. I. (2001) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.2001.281.6.e1340 · Cited by 561 · see citing works
Aging-associated reductions in AMP-activated protein kinase activity and mitochondrial biogenesis
Reznick, R. M., Zong, H., Li, J., Morino, K., Moore, I. K., Yu, H. J., Liu, Z.-X., Dong, J., Mustard, K. J., Hawley, S. A., et al. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.01.008 · Cited by 555 · see citing works
Self-citationMitohormesis: Promoting Health and Lifespan by Increased Levels of Reactive Oxygen Species (ROS)
Ristow, M., & Schmeisser, K. (2014) · Dose-Response · doi:10.2203/dose-response.13-035.ristow · Cited by 534 · see citing works
Laker, R. C., Drake, J. C., Wilson, R. J., Lira, V. A., Lewellen, B. M., Ryall, K. A., Fisher, C. C., Zhang, M., Saucerman, J. J., Goodyear, L. J., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00520-9 · Cited by 529 · see citing works
The Diabetes Prevention Program Research Group (2012) · Diabetes Care · doi:10.2337/dc11-1299 · Cited by 494 · see citing works
“AMPK is an important regulator of mitochondrial biogenesis (20), hepatic and muscle fatty acid oxidation, glucose transport, insulin secretion, and lipogenesis (21).”
BackgroundHolmes, B. F., Kurth-Kraczek, E. J., & Winder, W. W. (1999) · Journal of Applied Physiology · doi:10.1152/jappl.1999.87.5.1990 · Cited by 489 · see citing works
Self-citationSkeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance
Kiens, B. (2006) · Physiological Reviews · doi:10.1152/physrev.00023.2004 · Cited by 478 · see citing works
AMP kinase and malonyl-CoA: targets for therapy of the metabolic syndrome
Ruderman, N., & Prentki, M. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1344 · Cited by 451 · see citing works
Wojtaszewski, J. F. P., Nielsen, P., Hansen, B. F., Richter, E. A., & Kiens, B. (2000) · The Journal of Physiology · doi:10.1111/j.1469-7793.2000.t01-1-00221.x · Cited by 435 · see citing works
PGC-1α regulation by exercise training and its influences on muscle function and insulin sensitivity
Lira, V. A., Benton, C. R., Yan, Z., & Bonen, A. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00755.2009 · Cited by 412 · see citing works
Sensing of energy and nutrients by AMP-activated protein kinase
Hardie, D. G. (2011) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.001925 · Cited by 372 · see citing works
Self-citationMusi, N., Fujii, N., Hirshman, M. F., Ekberg, I., Fröberg, S., Ljungqvist, O., Thorell, A., & Goodyear, L. J. (2001) · Diabetes · doi:10.2337/diabetes.50.5.921 · Cited by 371 · see citing works
Effect of Exercise Intensity on Skeletal Muscle AMPK Signaling in Humans
Chen, Z.-P., Stephens, T. J., Murthy, S., Canny, B. J., Hargreaves, M., Witters, L. A., Kemp, B. E., & McConell, G. K. (2003) · Diabetes · doi:10.2337/diabetes.52.9.2205 · Cited by 369 · see citing works
Role of liver sinusoidal endothelial cells in non-alcoholic fatty liver disease
Hammoutene, A., & Rautou, P.-E. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.02.012 · Cited by 364 · see citing works
AMPK regulation of fatty acid metabolism and mitochondrial biogenesis: implications for obesity.
O’Neill, H. M., Holloway, G. P., & Steinberg, G. R. (2013) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2012.06.019 · Cited by 364 · see citing works
AMP-Activated Protein Kinase: An Ultrasensitive System for Monitoring Cellular Energy Charge
HARDIE, D. G., SALT, I. P., HAWLEY, S. A., & DAVIES, S. P. (1999) · Biochemical Journal · doi:10.1042/bj3380717 · Cited by 362 · see citing works
Hepatic lipid metabolism and non-alcoholic fatty liver disease
Tessari, P., Coracina, A., Cosma, A., & Tiengo, A. (2009) · Nutrition, Metabolism and Cardiovascular Diseases · doi:10.1016/j.numecd.2008.12.015 · Cited by 314 · see citing works
AMPK: Lessons from transgenic and knockout animals
Viollet, B. (2009) · Frontiers in bioscience · doi:10.2741/3229 · Cited by 314 · see citing works
Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects with Type 2 Diabetes
Sriwijitkamol, A., Coletta, D. K., Wajcberg, E., Balbontin, G. B., Reyna, S. M., Barrientes, J., Eagan, P. A., Jenkinson, C. P., Cersosimo, E., DeFronzo, R. A., et al. (2007) · Diabetes · doi:10.2337/db06-1119 · Cited by 304 · see citing works
YAMASHITA, H., FUJISAWA, K., ITO, E., IDEI, S., KAWAGUCHI, N., KIMOTO, M., HIEMORI, M., & TSUJI, H. (2007) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.60668 · Cited by 297 · see citing works
AMPK and Exercise: Glucose Uptake and Insulin Sensitivity
O'Neill, H. M. (2013) · Diabetes & Metabolism Journal · doi:10.4093/dmj.2013.37.1.1 · Cited by 295 · see citing works
Predominant α2/β2/γ3 AMPK activation during exercise in human skeletal muscle
Birk, J. B., & Wojtaszewski, J. F. P. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.120972 · Cited by 293 · see citing works
Bandyopadhyay, G. K., Yu, J. G., Ofrecio, J., & Olefsky, J. M. (2006) · Diabetes · doi:10.2337/db06-0062 · Cited by 291 · see citing works
Exercise and gene expression: physiological regulation of the human genome through physical activity
Booth, F. W., Chakravarthy, M. V., & Spangenburg, E. E. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2002.019265 · Cited by 277 · see citing works
Effects of α‐AMPK knockout on exercise‐induced gene activation in mouse skeletal muscle
Jørgensen, S. B., Wojtaszewski, J. F. P., Viollet, B., Andreelli, F., Birk, J. B., Hellsten, Y., Schjerling, P., Vaulont, S., Neufer, P. D., Richter, E. A., et al. (2005) · The FASEB Journal · doi:10.1096/fj.04-3144fje · Cited by 274 · see citing works
AMP-activated protein kinase: maintaining energy homeostasis at the cellular and whole-body levels
Hardie, D. G. (2014) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-071812-161148 · Cited by 281 · see citing works
Self-citationEgan, B., & Sharples, A. P. (2023) · Physiological Reviews · doi:10.1152/physrev.00054.2021 · Cited by 278 · see citing works
The Role of AMPK in the Regulation of Skeletal Muscle Size, Hypertrophy, and Regeneration
Thomson, D. M. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19103125 · Cited by 267 · see citing works
Wojtaszewski, J. F. P., Jørgensen, S. B., Hellsten, Y., Hardie, D. G., & Richter, E. A. (2002) · Diabetes · doi:10.2337/diabetes.51.2.284 · Cited by 261 · see citing works
Self-citationRole of AMPK in skeletal muscle metabolic regulation and adaptation in relation to exercise
Jørgensen, S. B., Richter, E. A., & Wojtaszewski, J. F. P. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.109942 · Cited by 261 · see citing works
Glucose, exercise and insulin: emerging concepts
Richter, E. A., Derave, W., & Wojtaszewski, J. F. P. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.t01-2-00313.x · Cited by 258 · see citing works
Engin, A. B. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-48382-5_8 · Cited by 260 · see citing works
Jørgensen, S. B., Nielsen, J. N., Birk, J. B., Olsen, G. S., Viollet, B., Andreelli, F., Schjerling, P., Vaulont, S., Hardie, D. G., Hansen, B. F., et al. (2004) · Diabetes · doi:10.2337/diabetes.53.12.3074 · Cited by 241 · see citing works
Self-citationRoepstorff, C., Thiele, M., Hillig, T., Pilegaard, H., Richter, E. A., Wojtaszewski, J. F. P., & Kiens, B. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.108720 · Cited by 238 · see citing works
Winder, W. W., Wilson, H. A., Hardie, D. G., Rasmussen, B. B., Hutber, C. A., Call, G. B., Clayton, R. D., Conley, L. M., Yoon, S., & Zhou, B. (1997) · Journal of Applied Physiology · doi:10.1152/jappl.1997.82.1.219 · Cited by 237 · see citing works
Self-citationThe Regulation of Fat Metabolism during Aerobic Exercise
Muscella, A., Stefàno, E., Lunetti, P., Capobianco, L., & Marsigliante, S. (2020) · Biomolecules · doi:10.3390/biom10121699 · Cited by 237 · see citing works
Role of Age-Related Mitochondrial Dysfunction in Sarcopenia
Ferri, E., Marzetti, E., Calvani, R., Picca, A., Cesari, M., & Arosio, B. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21155236 · Cited by 224 · see citing works
New strategies in sport nutrition to increase exercise performance
Close, G. L., Hamilton, D. L., Philp, A., Burke, L. M., & Morton, J. P. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.01.016 · Cited by 221 · see citing works
AMPK as a metabolic switch in rat muscle, liver and adipose tissue after exercise
Ruderman, N. B., Park, H., Kaushik, V. K., Dean, D., Constant, S., Prentki, M., & Saha, A. K. (2003) · Acta Physiologica Scandinavica · doi:10.1046/j.1365-201x.2003.01164.x · Cited by 217 · see citing works
The regulation of carbohydrate and fat metabolism during and after exercise
Holloszy, J. O. (1998) · Frontiers in bioscience · doi:10.2741/a342 · Cited by 180 · see citing works
Wojtaszewski, J. F. P., Birk, J. B., Frøsig, C., Holten, M., Pilegaard, H., & Dela, F. (2005) · The Journal of Physiology · doi:10.1113/jphysiol.2005.082669 · Cited by 170 · see citing works
AMP-activated protein kinase: a key system mediating metabolic responses to exercise
HARDIE, D. G. (2004) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000106171.38299.64 · Cited by 165 · see citing works
Self-citationSteinberg, G. R., O'Neill, H. M., Dzamko, N. L., Galic, S., Naim, T., Koopman, R., Jørgensen, S. B., Honeyman, J., Hewitt, K., Chen, Z.-P., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.102434 · Cited by 164 · see citing works
Unravelling the mechanisms regulating muscle mitochondrial biogenesis
Hood, D. A., Tryon, L. D., Carter, H. N., Kim, Y., & Chen, C. C. W. (2016) · Biochemical Journal · doi:10.1042/bcj20160009 · Cited by 159 · see citing works
Effect of exercise intensity on skeletal muscle malonyl-CoA and acetyl-CoA carboxylase
Rasmussen, B. B., & Winder, W. W. (1997) · Journal of Applied Physiology · doi:10.1152/jappl.1997.83.4.1104 · Cited by 158 · see citing works
Self-citationAMPK‐independent pathways regulate skeletal muscle fatty acid oxidation
Dzamko, N., Schertzer, J. D., Ryall, J. G., Steel, R., Macaulay, S. L., Wee, S., Chen, Z., Michell, B. J., Oakhill, J. S., Watt, M. J., et al. (2008) · The Journal of Physiology · doi:10.1113/jphysiol.2008.159814 · Cited by 147 · see citing works
Energy sensing by the AMP-activated protein kinase and its effects on muscle metabolism
Hardie, D. G. (2011) · Proceedings of the Nutrition Society · doi:10.1017/s0029665110003915 · Cited by 146 · see citing works
Self-citationThomson, D. M., Porter, B. B., Tall, J. H., Kim, H.-J., Barrow, J. R., & Winder, W. W. (2007) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00366.2006 · Cited by 146 · see citing works
Self-citationExercise-induced AMPK activity in skeletal muscle: Role in glucose uptake and insulin sensitivity
Friedrichsen, M., Mortensen, B., Pehmøller, C., Birk, J. B., & Wojtaszewski, J. F. P. (2013) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2012.06.013 · Cited by 143 · see citing works
Malonyl-CoA and the regulation of fatty acid oxidation in soleus muscle
ALAM, N., & SAGGERSON, E. D. (1998) · Biochemical Journal · doi:10.1042/bj3340233 · Cited by 141 · see citing works
Fat adaptation in well-trained athletes: effects on cell metabolism
Yeo, W. K., Carey, A. L., Burke, L., Spriet, L. L., & Hawley, J. A. (2011) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/h10-089 · Cited by 118 · see citing works
Using Molecular Biology to Maximize Concurrent Training
Baar, K. (2014) · Sports Medicine · doi:10.1007/s40279-014-0252-0 · Cited by 118 · see citing works
“Active AMPK is involved in the increase in fat oxidation during exercise [33] and also plays a role in the long-term regulation of mitochondrial mass by controlling the transcription and activity of PGC-1a [34].”
BackgroundMcGee, S. L., Mustard, K. J., Hardie, D. G., & Baar, K. (2008) · The Journal of Physiology · doi:10.1113/jphysiol.2007.143685 · Cited by 105 · see citing works
Self-citationSteinberg, G. R., Watt, M. J., McGee, S. L., Chan, S., Hargreaves, M., Febbraio, M. A., Stapleton, D., & Kemp, B. E. (2006) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/h06-003 · Cited by 104 · see citing works
Civitarese, A. E., Hesselink, M. K. C., Russell, A. P., Ravussin, E., & Schrauwen, P. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00193.2005 · Cited by 99 · see citing works
Putman, C. T., Kiricsi, M., Pearcey, J., MacLean, I. M., Bamford, J. A., Murdoch, G. K., Dixon, W. T., & Pette, D. (2003) · The Journal of Physiology · doi:10.1113/jphysiol.2003.040691 · Cited by 110 · see citing works
LKB1 and AMPK and the regulation of skeletal muscle metabolism
Koh, H.-J., Brandauer, J., & Goodyear, L. J. (2008) · Current Opinion in Clinical Nutrition and Metabolic Care · doi:10.1097/mco.0b013e3282fb7b76 · Cited by 78 · see citing works
Rasmussen, B. B., Hancock, C. R., & Winder, W. W. (1998) · Journal of Applied Physiology · doi:10.1152/jappl.1998.85.5.1629 · Cited by 78 · see citing works
Self-citationFunai, K., & Cartee, G. D. (2009) · Diabetes · doi:10.2337/db08-1477 · Cited by 76 · see citing works
Chronic elevated calcium blocks AMPK-induced GLUT-4 expression in skeletal muscle
Park, S., Scheffler, T. L., Gunawan, A. M., Shi, H., Zeng, C., Hannon, K. M., Grant, A. L., & Gerrard, D. E. (2009) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00114.2008 · Cited by 76 · see citing works
Miyamoto, L., Toyoda, T., Hayashi, T., Yonemitsu, S., Nakano, M., Tanaka, S., Ebihara, K., Masuzaki, H., Hosoda, K., Ogawa, Y., et al. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01034.2006 · Cited by 72 · see citing works
Roman, E. A. F. R., Reis, D., Romanatto, T., Maimoni, D., Ferreira, E. A., Santos, G. A., Torsoni, A. S., Velloso, L. A., & Torsoni, M. A. (2010) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2009.08.007 · Cited by 71 · see citing works
Cloning and characterization of mouse 5′-AMP-activated protein kinase γ3 subunit
Yu, H., Fujii, N., Hirshman, M. F., Pomerleau, J. M., & Goodyear, L. J. (2004) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00319.2003 · Cited by 67 · see citing works
Richter, E. A., Bilan, P. J., & Klip, A. (2025) · Physiological Reviews · doi:10.1152/physrev.00033.2024 · Cited by 64 · see citing works
Lefort, N., St.-Amand, E., Morasse, S., Côté, C. H., & Marette, A. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90362.2008 · Cited by 57 · see citing works
Optimizing training adaptations by manipulating glycogen
Baar, K., & McGee, S. (2008) · European Journal of Sport Science · doi:10.1080/17461390801919094 · Cited by 51 · see citing works
Mitochondrial and performance adaptations to exercise training in mice lacking skeletal muscle LKB1
Tanner, C. B., Madsen, S. R., Hallowell, D. M., Goring, D. M. J., Moore, T. M., Hardman, S. E., Heninger, M. R., Atwood, D. R., & Thomson, D. M. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00227.2013 · Cited by 47 · see citing works
Hormones, Metabolism and the Benefits of Exercise
(2017) · Research and Perspectives in Endocrine Interactions · doi:10.1007/978-3-319-72790-5 · Cited by 44 · see citing works
Protein Intake Does Not Increase Vastus Lateralis Muscle Protein Synthesis during Cycling
HULSTON, C. J., WOLSK, E., GRØNDAHL, T. S., YFANTI, C., & VAN HALL, G. (2011) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e31821661ab · Cited by 32 · see citing works
Biological mechanisms of stroke prevention by physical activity in type 2 diabetes
Agosti, V., Graziano, S., Artiaco, L., & Sorrentino, G. (2009) · Acta Neurologica Scandinavica · doi:10.1111/j.1600-0404.2008.01080.x · Cited by 25 · see citing works
Frøsig, C., Roepstorff, C., Brandt, N., Maarbjerg, S. J., Birk, J. B., Wojtaszewski, J. F. P., Richter, E. A., & Kiens, B. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90556.2008 · Cited by 21 · see citing works
- Authors
- Winder, W. W., & Hardie, D. G.
- Affiliations
- Brigham Young University (US)
- ORCID
- Hardie, D. G.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 1996-02-01
- Volume
- 270
- Issue
- 2
- Pages
- E299-E304
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2169469988
- Cited by
- 799
- References
- 24
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 8 · 2023: 11 · 2022: 15 · 2021: 24 · 2020: 24 · 2019: 24 · 2018: 28 · 2017: 28 · 2016: 29 · 2015: 46 · 2014: 34 · 2013: 30 · 2012: 39
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR041438, AR-41438)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- acetyl-CoA carboxylase, AMPK, malonyl-CoA, skeletal muscle, physical activity, fatty acid oxidation, enzyme phosphorylation, treadmill running, fast-twitch muscle, citrate, energy metabolism regulation
- MeSH terms
- Acetyl-CoA Carboxylase, Animals, Enzyme Activation, Male, Malonyl Coenzyme A, Motor Activity, Multienzyme Complexes, Phosphorylation, Protein Kinases, Rats, Sprague-Dawley, Protein Serine-Threonine Kinases, Muscle, Skeletal, Rats, AMP-Activated Protein Kinases, In Vitro Techniques
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