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Growth factors immediately raise cytoplasmic free Ca2+ in human fibroblasts.
Open accessAbstract
Addition of platelet-derived growth factor, epidermal growth factor, or serum to quiescent human fibroblasts, loaded with the fluorescent Ca2+ indicator quin-2, causes an immediate, up to 3-fold, rise in cytoplasmic free Ca2+ concentration [( Ca2+]i). In contrast, insulin and tumor-promoting phorbol ester have no effect on [Ca2+]i. The mitogen-induced [Ca2+]i response is initiated within a few s, reaches a maximum by 20-40 s, and then slowly declines to a new steady level. The [Ca2+]i response is not prevented by removal of external Ca2+ and is independent of the transmembrane Na+ gradient and membrane potential. It is concluded that platelet-derived growth factor, epidermal growth factor, and serum rapidly mobilize Ca2+ from intracellular stores, presumably due to the prior breakdown of inositol phospholipids, and that the resulting rise in [Ca2+]i may function as an initial signal in growth factor action.
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Cited by
Inositol Trisphosphate, a Novel Second Messenger in Cellular Signal Transduction
Berridge, M. J., & Irvine, R. F. (1984) · Nature · doi:10.1038/312315a0 · Cited by 6,086
Early Signals in the Mitogenic Response
Rozengurt, E. (1986) · Science · doi:10.1126/science.3018928 · Cited by 1,455
Hauschka, P. V., Mavrakos, A. E., Iafrati, M. D., Doleman, S. E., & Klagsbrun, M. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67143-1 · Cited by 869
Induction of Membrane Ruffling and Fluid-Phase Pinocytosis in Quiescent Fibroblasts by ras Proteins
Bar-Sagi, D., & Feramisco, J. R. (1986) · Science · doi:10.1126/science.3090687 · Cited by 734
Arslan, P., Di Virgilio, F., Beltrame, M., Tsien, R. Y., & Pozzan, T. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89421-2 · Cited by 485
Metadata
- Authors
- Moolenaar, W. H., Tertoolen, L. G., & de Laat, S. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-07
- Volume
- 259
- Issue
- 13
- Pages
- 8066-8069
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1489663455
- Cited by
- 391
- References
- 0
- FWCI
- 21.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Moolenaar, W. H., Tertoolen, L. G., & de Laat, S. W. (1984). Growth factors immediately raise cytoplasmic free Ca2+ in human fibroblasts. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)39690-4
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