Cited by 79Cholesterol and Lipid MetabolismLipid Membrane Structure and BehaviorLipid metabolism and biosynthesis

Cholesterol movement from plasma membrane to rough endoplasmic reticulum. Inhibition by progesterone.

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Lange, Y.·1994·Journal of Biological Chemistry

Abstract

The effect of progesterone on the movement of sterols from the cell surface to the rough endoplasmic reticulum (ER) was examined in rat hepatoma cells. Plasma membranes were labeled exogenously with [3H]cholesterol or [3H]zymosterol. Translocation of the labeled sterols to the rough ER was inferred from their conversion to [3H]cholesteryl esters and [3H]cholesterol, respectively. Progesterone inhibited both of these reactions by more than 90%. The concentration for half-maximal inhibition was 0.7 microgram/ml. Progesterone did not inhibit acyl-CoA:cholesterol acyltransferase activity itself, since the steroid had no effect on the esterification of [3H]cholesterol synthesized in vitro in the rough ER from [3H]zymosterol. Moreover, the small amount of [3H]cholesterol synthesized from plasma membrane [3H]zymosterol in progesterone-treated intact cells was esterified at the same fractional rate as cholesterol in control cells. Subcellular fractionation of cells pulse-labeled with [3H]cholesterol and treated with progesterone suggested that the block in plasma membrane cholesterol transfer to the rough ER occurred at the level of the plasma membrane.

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Crossref · 10.1016/s0021-9258(17)41877-1

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Authors
Lange, Y.
Affiliations
Rush University Medical Center (US)
Corresponding author
Lange, Y.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-02
Volume
269
Issue
5
Pages
3411-3414
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1518063279
Cited by
79
References
15
Citations by year
2025: 1 · 2023: 1 · 2022: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2
FWCI
10.97
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL-28448)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
cholesterol translocation, rough endoplasmic reticulum, progesterone, plasma membrane, sterol esterification, acyl-CoA:cholesterol acyltransferase, zymosterol, hepatoma cells, cholesterol biosynthesis
MeSH terms
Animals, Biological Transport, Carbon Radioisotopes, Cell Membrane, Cholesterol, Cholesterol Esters, Dose-Response Relationship, Drug, Endoplasmic Reticulum, Kinetics, Liver Neoplasms, Liver Neoplasms, Experimental, Progesterone, Radioisotope Dilution Technique, Sterols, Tritium, Tumor Cells, Cultured, Rats

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