Cholesterol movement from plasma membrane to rough endoplasmic reticulum. Inhibition by progesterone.
Open accessAbstract
The effect of progesterone on the movement of sterols from the cell surface to the rough endoplasmic reticulum (ER) was examined in rat hepatoma cells. Plasma membranes were labeled exogenously with [3H]cholesterol or [3H]zymosterol. Translocation of the labeled sterols to the rough ER was inferred from their conversion to [3H]cholesteryl esters and [3H]cholesterol, respectively. Progesterone inhibited both of these reactions by more than 90%. The concentration for half-maximal inhibition was 0.7 microgram/ml. Progesterone did not inhibit acyl-CoA:cholesterol acyltransferase activity itself, since the steroid had no effect on the esterification of [3H]cholesterol synthesized in vitro in the rough ER from [3H]zymosterol. Moreover, the small amount of [3H]cholesterol synthesized from plasma membrane [3H]zymosterol in progesterone-treated intact cells was esterified at the same fractional rate as cholesterol in control cells. Subcellular fractionation of cells pulse-labeled with [3H]cholesterol and treated with progesterone suggested that the block in plasma membrane cholesterol transfer to the rough ER occurred at the level of the plasma membrane.
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25-HC lacks the membrane-ordering and packing capacities of cholesterol, altering membrane fluidity upon incorporation.
“25-HC lacks the membrane-ordering and packing capacities of cholesterol, thus membrane fluidity is altered upon its incorporation49,50.”
From Peroxisomal very long-chain fatty acid transport is targeted by herpesviruses and the antiviral host response (2022) · cites this paper as [50]
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- Authors
- Lange, Y.
- Affiliations
- Rush University Medical Center (US)
- Corresponding author
- Lange, Y.
- ORCID
- Lange, Y.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-02
- Volume
- 269
- Issue
- 5
- Pages
- 3411-3414
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1518063279
- Cited by
- 79
- References
- 15
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2
- FWCI
- 10.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL-28448)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- cholesterol translocation, rough endoplasmic reticulum, progesterone, plasma membrane, sterol esterification, acyl-CoA:cholesterol acyltransferase, zymosterol, hepatoma cells, cholesterol biosynthesis
- MeSH terms
- Animals, Biological Transport, Carbon Radioisotopes, Cell Membrane, Cholesterol, Cholesterol Esters, Dose-Response Relationship, Drug, Endoplasmic Reticulum, Kinetics, Liver Neoplasms, Liver Neoplasms, Experimental, Progesterone, Radioisotope Dilution Technique, Sterols, Tritium, Tumor Cells, Cultured, Rats
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