Cited by 190Genomics and Chromatin DynamicsEstrogen and related hormone effectsChromatin Remodeling and Cancer

Early events in the stimulation of mammary tumor virus RNA synthesis by glucocorticoids. Novel assays of transcription rates.

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Ucker, D S, Yamamoto, K R·1984·Journal of Biological Chemistry

Abstract

Two procedures were devised to examine initial events in the glucocorticoid-regulated transcription of integrated murine mammary tumor virus (MTV) DNA sequences. First, cells in monolayer culture were exposed for brief periods to dexamethasone and then permeabilized in situ with digitonin to allow entry of 32P-labeled ribonucleoside triphosphates and measurement of transcription rates. The results revealed that the rate of MTV RNA synthesis is stimulated selectively by dexamethasone after an apparent lag of approximately equal to 1 min and is half-maximal by 8-9 min. Second, the time course of transcription elongation was monitored by quantitating in single strand nuclease protection assays the accumulation of RNA sequences from different regions of the 7.8-kilobase MTV transcription unit. These experiments suggested that the rate of MTV RNA elongation in the presence of hormone does not differ substantially from elongation rates calculated for other genes transcribed by RNA polymerase II. Moreover, dexamethasone increased in parallel and to the same extent the levels of RNA from various segments of the transcription unit, implying that transcripts are elongated across the MTV element at a constant rate. We conclude that glucocorticoids stimulate MTV transcription solely via a rapid and selective increase in the efficiency of transcription initiation.

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Crossref · 10.1016/s0021-9258(17)42806-7

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  • Pioneering biochemical studies estimated average RNA Pol II speed between 1.1 kb/min and 4.8 kb/min on a few genes.

    “By monitoring the appearance of RNA by in situ hybridization, nuclear run‐on, slot blot, or RT–PCR after expression activation, RNA Pol II speed was estimated between 1.1 kb/min and 4.8 kb/min (Ucker & Yamamoto, 1984; Thummel et al, 1990; Shermoen & O'Farrell, 1991; O'Brien & Lis, 1993; Tennyson et al, 1995; Femino et al, 1998; Femino et al, 2003; Hanisch et al, 2013).”

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Authors
Ucker, D. S., & Yamamoto, K. R.
Affiliations
University of California, San Francisco (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1984-06
Volume
259
Issue
12
Pages
7416-7420
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1483243107
Cited by
190
References
28
Citations by year
2022: 2 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
FWCI
5.31
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
Keywords
mammary tumor virus, glucocorticoids, dexamethasone, transcription initiation, transcription elongation, RNA polymerase II, transcription rate, transcriptional regulation, nuclease protection assay, digitonin permeabilization
MeSH terms
Animals, Base Sequence, Dexamethasone, Digitonin, Endonucleases, Mammary Tumor Virus, Mouse, Nucleic Acid Hybridization, RNA, Viral, Time Factors, Transcription, Genetic, Single-Strand Specific DNA and RNA Endonucleases, Rats

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