Cited by 201Adipose Tissue and MetabolismPeroxisome Proliferator-Activated ReceptorsLipid metabolism and biosynthesis

The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.

Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture

Open access

Rehnmark, S, Néchad, M, Herron, D, Cannon, B, Nedergaard, J·1990·Journal of Biological Chemistry

Open this paper in CoChat — free account

Abstract

In order to examine the control of expression of the gene coding for the brown fat specific uncoupling protein thermogenin (UCP), brown fat cells isolated as undifferentiated precursors from the interscapular brown adipose tissue of young mice were grown in culture. In these cells, it was possible by norepinephrine (NE) addition to induce specifically the expression of the UCP gene. The effect of NE was due to activation of transcription. The ability to express the UCP gene was maximal in cells around confluence; cell cultures younger or older than this showed a lower response. The response to NE showed a sharp optimum around 0.1 microM and was linear with time over the 4-h period studied. The presence of insulin or thyroid hormones facilitated the NE response. Pharmacological analysis of the adrenergic response indicated that UCP gene expression could be induced both via beta-receptors (probably beta 3) and via alpha 1-receptors; these effects were synergistic. It was concluded that it is possible to promote these precursor cells to advance to such a state of differentiation that they can demonstrate the selective feature of the brown fat cell, i.e. the ability to express UCP. The expression of the UCP gene is regulated via interacting adrenergic mechanisms.

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(17)46245-4

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Differentiation of Receptor Systems activated by Sympathomimetic Amines
Lands, A. M., ARNOLD, A., McAuliff, J. P., Ludueña, F. P., & Brown, T. G. (1967) · Nature · doi:10.1038/214597a0 · Cited by 2,061
Atypical β-adrenoceptor on brown adipocytes as target for anti-obesity drugs
Arch, J. R. S., Ainsworth, A. T., Cawthorne, M. A., Piercy, V., Sennitt, M. V., Thody, V. E., Wilson, C., & Wilson, S. (1984) · Nature · doi:10.1038/309163a0 · Cited by 728
Intracellular conversion of thyroxine to triiodothyronine is required for the optimal thermogenic function of brown adipose tissue.
Bianco, A. C., & Silva, J. E. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112798 · Cited by 347
The biochemistry of an inefficient tissue: brown adipose tissue.
Cannon, B., & Nedergaard, J. (1985) · PubMed · Cited by 309
Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression
Jacobsson, A., Stadler, U., Glotzer, M. A., & Kozak, L. P. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36228-2 · Cited by 300
CGP-12177. A hydrophilic beta-adrenergic receptor radioligand reveals high affinity binding of agonists to intact cells.
STAEHELIN, M., Simons, P., Jaeggi, K., & Wigger, N. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32689-9 · Cited by 288
Expression of uncoupling protein mRNA in thermogenic or weakly thermogenic brown adipose tissue. Evidence for a rapid beta-adrenoreceptor-mediated and transcriptionally regulated step during activation of thermogenesis.
Ricquier, D., Bouillaud, F., Toumelin, P. L., Mory, G., Bazin, R., Arch, J. R. S., & Pénicaud, L. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66957-1 · Cited by 234
Triiodothyronine amplifies norepinephrine stimulation of uncoupling protein gene transcription by a mechanism not requiring protein synthesis.
Bianco, A. C., Sheng, X. Y., & Silva, J. E. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)81340-6 · Cited by 205
Hexose Transport in Isolated Brown Fat Cells
Czech, M., Lawrence, J. C., & Lynn, W. S. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)79744-9 · Cited by 159
Development of brown fat cells in monolayer culture
Néchad, M., Kuusela, P., Carneheim, C., Björntorp, P. A., Nedergaard, J., & Cannon, B. (1983) · Experimental Cell Research · doi:10.1016/0014-4827(83)90384-1 · Cited by 154
Increased level of mRNA for the uncoupling protein in brown adipose tissue of rats during thermogenesis induced by cold exposure or norepinephrine infusion.
Bouillaud, F., Ricquier, D., Mory, G., & Thibault, J. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)90902-6 · Cited by 142
The brown adipocyte β-adrenoceptor
Arch, J. R. S. (1989) · Proceedings of The Nutrition Society · doi:10.1079/pns19890032 · Cited by 124
The β-adrenergic radioligand [3H]CGP-12177, generally classified as an antagonist, is a thermogenic agonist in brown adipose tissue
Mohell, N., & Dicker, A. (1989) · Biochemical Journal · doi:10.1042/bj2610401 · Cited by 115
Rapid increase of mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue
Ricquier, D., Mory, G., Bouillaud, F., Thibai, J., & Weissenbach, J. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)80608-0 · Cited by 96
The relative contribution of affinity and efficacy to agonist activity: organ selectivity of noradrenaline and oxymetazoline with reference to the classification of drug receptors
Kenakin, T. (1984) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1984.tb10753.x · Cited by 91
Brown adipocytes differentiated in vitro can express the gene for the uncoupling protein thermogenin: Effects of hypothyroidism and norepinephrine
Rehnmark, S., Kopecký, J., Jacobsson, A., Néchad, M., Herron, D. K., Nelson, B. D., Obregón, M.-J., Nedergaard, J., & Cannon, B. (1989) · Experimental Cell Research · doi:10.1016/0014-4827(89)90280-2 · Cited by 76
Quantitative differentiation of α- and β-adrenergic respiratory responses in isolated hamster brown fat cells: Evidence for the presence of an α1-adrenergic component
Mohell, N., Nedergaard, J., & Cannon, B. (1983) · European Journal of Pharmacology · doi:10.1016/0014-2999(83)90136-x · Cited by 75
Norepinephrine‐Stimulated Fatty‐Acid Release and Oxygen Consumption in Isolated Hamster Brown‐Fat Cells
Nedergaard, J., & Lindberg, O. (1979) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1979.tb12948.x · Cited by 72
α1- and β-Adrenergic Agents Cause Synergistic Stimulation of the Iodothyronine Deiodinase in Rat Brown Adipocytes*
Raasmaja, A., & Larsen, P. R. (1989) · Endocrinology · doi:10.1210/endo-125-5-2502 · Cited by 69
Uncoupling protein in embryonic brown adipose tissue — existence of nonthermogenic and thermogenic mitochondria
Houštěk, J., Kopecký, J., Rychter, Z., & Soukup, T. (1988) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(88)90103-x · Cited by 69
Noradrenergic stimulation of mitochondriogenesis in brown adipocytes differentiating in culture
Néchad, M., Nedergaard, J., & Cannon, B. (1987) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1987.253.6.c889 · Cited by 69
α- and β-adrenergic control of thermogenin mRNA expression in brown adipose tissue
Jacobsson, A., Nedergaard, J., & Cannon, B. (1986) · Bioscience Reports · doi:10.1007/bf01114756 · Cited by 68
A preadipocyte clonal line from mouse brown adipose tissue
Forest, C. D., Doglio, A., Ricquier, D., & Ailhaud, G. P. (1987) · Experimental Cell Research · doi:10.1016/0014-4827(87)90430-7 · Cited by 61
Characterization of the beta-adrenoceptor of the adipose cell of the rat.
Tan, S., & Curtis‐Prior, P. B. (1983) · PubMed · Cited by 57
High Number of High-Affinity Binding Sites for (-)-[3H]Dihydroalprenolol on Isolated Hamster Brown-Fat Cells. A Study of the beta-Adrenergic Receptors
Svoboda, P., Svartengren, J., Snochowski, M., Houštěk, J., & Cannon, B. (1979) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1979.tb06281.x · Cited by 57
Beta-1 receptor is the predominant beta-adrenoreceptor on rat brown adipose tissue.
Levin, B. E., & Sullivan, A. C. (1986) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)38950-0 · Cited by 50
Development of brown fat cells in monolayer culture
Néchad, M. (1983) · Experimental Cell Research · doi:10.1016/0014-4827(83)90385-3 · Cited by 47
Postnatal recruitment of brown adipose tissue is induced by the cold stress experienced by the pups. An analysis of mRNA levels for thermogenin and lipoprotein lipase
Obregón, M.-J., Jacobsson, A., Kirchgessner, T. G., Schotz, M. C., Cannon, B., & Nedergaard, J. (1989) · Biochemical Journal · doi:10.1042/bj2590341 · Cited by 47
Euthyroid status is essential for the perinatal increase in thermogenin mRNA in brown adipose tissue of rat pups
Obregón, M.-J., Pitamber, R., Jacobsson, A., Nedergaard, J., & Cannon, B. (1987) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(87)91069-2 · Cited by 46
Distinction between mechanisms underlying alpha 1- and beta-adrenergic respiratory stimulation in brown fat cells
Mohell, N., Connolly, E. D., & Nedergaard, J. (1987) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1987.253.2.c301 · Cited by 45
Physiological activation of brown adipose tissue destabilizes thermogenin mRNA
Jacobsson, A., Cannon, B., & Nedergaard, J. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80483-0 · Cited by 44
Modulation of β‐oxidation and proton conductance pathway of brown adipose tissue in hypo‐ and hyperinsulinemic states
Seydoux, J., Trimble, E. R., Bouillaud, F., Assimacopoulos‐Jeannet, F., Baş, S., Ricquier, D., Giacobino, J., & Girardier, L. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)80060-5 · Cited by 40
Expression of the mitochondrial uncoupling protein in brown adipocytes
Forest, C. D., Doglio, A., Casteilla, L., Ricquier, D., & Ailhaud, G. P. (1987) · Experimental Cell Research · doi:10.1016/0014-4827(87)90431-9 · Cited by 35
Insulin modifies the properties of glucose transporters in rat brown adipose tissue
Greco-Perotto, R., Assimacopoulos‐Jeannet, F., & Jeanrenaud, B. (1987) · Biochemical Journal · doi:10.1042/bj2470063 · Cited by 34
Norepinephrine-induced Na+ influx in brown adipocytes is cyclic AMP-mediated.
Connolly, E. D., Nånberg, E., & Nedergaard, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66880-2 · Cited by 33
Brown Fat Thermoregulation in Developing Hamsters (Mesocricetus auratus): a GDP-Binding Study
Sundin, U., Herron, D., & Cannon, B. (2009) · Biology of the Neonate · doi:10.1159/000241419 · Cited by 32
Induction of functional uncoupling protein in guinea pigs infused with noradrenaline. Studies with isolated brown adipocytes
Cunningham, S. A., & Nicholls, D. G. (1987) · Biochemical Journal · doi:10.1042/bj2450485 · Cited by 29
β-adrenergic stimulation of fatty acid release from brown fat cells differentiated in monolayer culture
Kuusela, P., Nedergaard, J., & Cannon, B. (1986) · Life Sciences · doi:10.1016/0024-3205(86)90052-4 · Cited by 29
Norepinephrine infusions increase adenylate cyclase responsiveness in brown adipose tissue.
Granneman, J. G. (1988) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)24074-5 · Cited by 27
Adrenergic regulation of the uncoupling protein expression in foetal rat brown adipocytes in primary culture
Porrás, A., Fernández, M., & Benito, M. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(89)92171-2 · Cited by 26
Potentiation of in vivo thermogenesis in rat brown adipose tissue by stimulation of α1-adrenoreceptors is associated with increased release of cyclic AMP
Ma, S. W. Y., & Foster, D. O. (1984) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/y84-158 · Cited by 25
Isolation and ultrastructural features of brown adipocytes in culture.
CIGOLINI, M., Cinti, S., Bosello, O., Brunetti, L., & Björntorp, P. A. (1986) · Europe PMC (PubMed Central) · Cited by 24
Effects of cholera toxin on gene expression in brown preadipocytes differentiating in culture
Herron, D. K., Néchad, M., Rehnmark, S., Nelson, B. D., Nedergaard, J., & Cannon, B. (1989) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1989.257.5.c920 · Cited by 24
Immunological detection of cDNA clones encoding the uncoupling protein of brown adipose tissue: Evidence for an antigenic determinant within the C-terminal eleven amino acids
Ridley, R. G., Patel, H. V., Parfett, C. L. J., Olynyk, K. A., Reichling, S., & Freeman, K. B. (1986) · Bioscience Reports · doi:10.1007/bf01145183 · Cited by 20
Hormonal Sensitivity of Brown Fat Cells of Fetal Rats in Monolayer Culture
Sugihara, H., Miyabara, S., Yonemitsu, N., & Ohta, K. (2009) · Experimental and Clinical Endocrinology & Diabetes · doi:10.1055/s-0029-1210291 · Cited by 17
Development of interscapular brown adipose tissue in the hamster. II ‐ Differentiation of transplants in the anterior chamber of the eye: role of the sympathetic innervation
Néchad, M., & Olson, L. (1984) · Biology of the Cell · doi:10.1111/j.1768-322x.1984.tb00211.x · Cited by 14
Some Aspects of Rat Metabolism in the Brown Adipose Tissue of Normal and Hypothyroid Rats during Early Postnatal Development
Hemon, P. (2009) · Biology of the Neonate · doi:10.1159/000240823 · Cited by 12
Effects of exposure temperature on brown adipose tissue uncoupling protein mRNA levels
Freeman, K. B., Heffernan, M., Dhalla, Z., & Patel, H. V. (1989) · Biochemistry and Cell Biology · doi:10.1139/o89-022 · Cited by 11
Effect of the α-Adrenoreceptor Agonist Ld 3098 (Cirazoline) on the Magnitude of End-Organ Responses Induced by Nerve Stimulation and Exogenous Noradrenaline in a Variety of Sympathetically Innervated Animal Tissues
Massingham, R., Dubocovich, M. L., & Langer, S. Z. (1979) · Clinical Science · doi:10.1042/cs057185s · Cited by 6

Research works printed but not linked: 8

Cannon, 1985, Essays Biochem., 20, 110
Cigolini, 1986, J. Anat., 145, 207
Sundin, 1980, Comp. Biochem. Physiol., 65B, 463
Houstek, 1989, Biochim. Biophys. Acta
Tan, 1983, Int. J. Obes., 7, 409
Mohell, 1989, Comp. Biochem. Physiol., 94C, 000
Burgi, 1986, Am. J. Physiol., 251, E503
Rothwell, 1985, Am. J. Physiol., 248, E397

Sorted by citations

A Cold-Inducible Coactivator of Nuclear Receptors Linked to Adaptive Thermogenesis
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M., & Spiegelman, B. M. (1998) · Cell · doi:10.1016/s0092-8674(00)81410-5 · Cited by 3,864
Occurrence of brown adipocytes in rat white adipose tissue: molecular and morphological characterization
Cousin, B., Cinti, S., Morroni, M., Raimbault, S., Ricquier, D., Pénicaud, L., & Casteilla, L. (1992) · Journal of Cell Science · doi:10.1242/jcs.103.4.931 · Cited by 665
Fat cell adrenergic receptors and the control of white and brown fat cell function
Lafontan, M., & Berlan, M. (1993) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37695-1 · Cited by 644
UCP1: The only protein able to mediate adaptive non-shivering thermogenesis and metabolic inefficiency
Nedergaard, J., Golozoubova, V., Matthias, A., Asadi, A., Jacobsson, A., & Cannon, B. (2001) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(00)00247-4 · Cited by 615
Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases
Demine, S., Renard, P., & Arnould, T. (2019) · Cells · doi:10.3390/cells8080795 · Cited by 476
“These molecules are also known to upregulate UCP-1 expression in many models, including mouse [40,41], rat [42,43], and human [44] brown adipose tissues.”
Background

See all 201

Authors: Rehnmark, S., Néchad, M., Herron, D., Cannon, B., & Nedergaard, J.
Affiliations: Stockholm University (SE)
ORCID: Rehnmark, S
Cannon, B
Nedergaard, J
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1990-09
Volume: 265
Issue: 27
Pages: 16464-16471
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1016/s0021-9258(17)46245-4
OpenAlex: W1501975414
Cited by: 201
References: 49
Citations by year: 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 5 · 2012: 3
FWCI: 6.98
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 11015 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: thermogenin, uncoupling proteins, brown adipocytes, brown adipose tissue, adipogenesis, norepinephrine, alpha1-adrenoceptors, beta3-adrenergic receptor, gene expression regulation, transcriptional activation, insulin, thyroid hormones
MeSH terms: Uncoupling Protein 1, Adrenergic beta-Antagonists, Animals, Adipose Tissue, Brown, Carrier Proteins, Cells, Cultured, Colforsin, Insulin, Ion Channels, Male, Membrane Proteins, Norepinephrine, Nucleic Acid Hybridization, Oxymetazoline, Phenylephrine, Prazosin, Propanolamines, Propranolol, Receptors, Adrenergic, alpha, Receptors, Adrenergic, beta, RNA, RNA, Messenger, Thyroxine, Triiodothyronine, 8-Bromo Cyclic Adenosine Monophosphate, Blotting, Northern, Gene Expression, Mitochondrial Proteins, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks